A5067442450- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Advanced Fiber Laser Technologies
- Optical Coherence Tomography Applications
- Photonic Crystal and Fiber Optics
- Cell Image Analysis Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Laser-Matter Interactions and Applications
- Nanoplatforms for cancer theranostics
- Chalcogenide Semiconductor Thin Films
- Solid-state spectroscopy and crystallography
- Advanced Fiber Optic Sensors
- Extracellular vesicles in disease
- Optical Network Technologies
- Optical Imaging and Spectroscopy Techniques
- Advanced Optical Sensing Technologies
- Cancer Cells and Metastasis
- Quantum Dots Synthesis And Properties
- Radiomics and Machine Learning in Medical Imaging
- Photodynamic Therapy Research Studies
- AI in cancer detection
- Mass Spectrometry Techniques and Applications
- Fluid Dynamics and Heat Transfer
- Medical Imaging Techniques and Applications
- Image Processing Techniques and Applications
University of Illinois Urbana-Champaign
2016-2025
University of Illinois System
2017-2023
Illinois College
2019-2021
First Affiliated Hospital of Fujian Medical University
2019
Fujian Medical University
2019
University of Electronic Science and Technology of China
2015-2016
University of Kentucky
1998-2008
University of California, Merced
2004-2007
Kansas State University
2003
Abstract Intravital microscopy (IVM) emerged and matured as a powerful tool for elucidating pathways in biological processes. Although label-free multiphoton IVM is attractive its non-perturbative nature, wide application has been hindered, mostly due to the limited contrast of each imaging modality challenge integrate them. Here we introduce simultaneous autofluorescence-multiharmonic (SLAM) microscopy, single-excitation source nonlinear platform that uses custom-designed excitation window...
Abstract Biophotonics and nonlinear fiber optics have traditionally been two independent fields. Since the discovery of fiber‐based supercontinuum generation in 1999, biophotonics applications employing incoherent light experienced a large impact from optics, primarily because access to wide range wavelengths uniform spatial profile afforded by supercontinuum. However, coherent not benefited most well‐known techniques for reasons such as poor coherence (or high noise), insufficient...
The notion of personalized medicine demands proper prognostic biomarkers to guide the optimal therapy for an invasive breast cancer patient. However, various risk prediction models based on conventional clinicopathological factors and emergent molecular assays have been frequently limited by either a low strength prognosis or restricted applicability specific types patients. Therefore, there is critical need develop strong general prognosticator.
Relative intensity noise (RIN) is one of the most significant factors limiting sensitivity an optical coherence tomography (OCT) system. The existing and prevalent theory being used for estimating RIN various light sources in OCT questionable, cannot be applied uniformly different types sources. origin differs significantly, owing to physical nature photon generation. In this study, we characterize compare several including superluminescent diodes (SLDs), erbium-doped fiber amplifier,...
Intraoperative imaging of tumor microenvironment reveals correlations between extracellular vesicle density and breast pathology.
Despite extensive interest, extracellular vesicle (EV) research remains technically challenging. One of the unexplored gaps in EV has been inability to characterize spatially and functionally heterogeneous populations EVs based on their metabolic profile. In this paper, we utilize intrinsic optical structural contrast demonstrate vivo/in situ characterization a variety unprocessed (pre)clinical samples. With pixel-level segmentation mask provided by deep neural network, individual can be...
We have examined charge stability limits of single evaporating microdroplets that were suspended in an electrodynamic balance. A high precision light scattering technique based on optical resonances was used to determine the size and change a droplet at instability induced breakup. The level loss breakup obtained from dc voltages required balance prior following results droplets diethyl phthalate (DEP), diethylene glycol (DEG), triethylene (TEG), hexadecane show breakups due occur Rayleigh...
Wave-breaking often occurs when a short intense optical pulse propagates in long normally dispersive fiber. This effect has conventionally been avoided fiber (super-)continuum-based compression because the accumulated frequency chirp of output cannot be fully compensated by standard prism (or grating) pair. Thus, spectral extending capability wave-breaking not utilized to shorten compressed pulse. We demonstrate that wave-breaking-free operation is necessary if 4f shaper-based compressor...
The supercontinuum generated exclusively in the normal dispersion regime of a nonlinear fiber is widely believed to possess low optical noise and high spectral coherence. recent development flattened all-normal fibers has been motivated by this belief construct general-purpose broadband coherent source. Somewhat surprisingly, we identify large short-term polarization type generation that masked total-intensity measurement past, but can be easily detected filtering with linear polarizer....
Spectrally-isolated narrowband Cherenkov radiation from commercial nonlinear photonic crystal fibers is demonstrated as an ultrafast optical source with a visible tuning range of 485-690 nm, which complementarily extends the near-infrared 690-1020 nm corresponding femtosecond Ti:sapphire pump laser. Pump-tosignal conversion efficiency routinely surpasses 10%, enabling multimilliwatt output across entire range. Appropriate selection fiber parameters and pumping conditions efficiently...
Otitis media (OM) is the most common illness in children United States.Three-fourths of under age three have OM at least once.Children with chronic OM, including effusion and recurrent will often conductive hearing loss communication difficulties, need surgical treatment.Recent clinical studies provide evidence that almost all cases are accompanied by a bacterial biofilm behind tympanic membrane (eardrum) within middle ear.Biofilms typically very thin, cannot be recognized using regular...
Dispersion-flattened dispersion-decreased all-normal dispersion (DFDD-ANDi) photonic crystal fibers have been identified as promising candidates for high-spectral-power coherent supercontinuum (SC) generation.However, the effects of unintentional birefringence on SC generation ignored.This is widely present in nonlinear non-polarization maintaining with a typical core size 2 µm, presumably due to structural symmetry breaks introduced fiber drawing process.We find that an intrinsic...
The generation of wavelengths above 3 μm by nonlinear processes in short silica photonic crystal fibers is investigated numerically. It was found that the 3-3.5 range may be generated quite efficiently centimeter-long fiber pieces when pumping with femtosecond pulses 1.55-2 range. Wavelengths 3.5-4 can principle generated, but these require shorter lengths for efficient extraction. results indicate useful sources fabricated existing silica-based technology.
Label-free molecular imaging of the tumor microenvironment interrelates well-known events in carcinogenesis across spatial scales.
Recent advances in label-free virtual histology promise a new era for real-time molecular diagnosis the operating room and during biopsy procedures. To take full advantage of rich, multidimensional information provided by these technologies, reproducible reliable computational tools that could facilitate are great demand. In this study, we developed deep-learning-based framework to recognize cancer versus normal human breast tissue from images, with tile-level AUC (area under receiver curve)...
Abstract Label-free nonlinear microscopy enables nonperturbative visualization of structural and metabolic contrast within living cells in their native tissue microenvironment. Here a computational pipeline was developed to provide quantitative view the microenvironmental architecture cancerous from label-free images. To enable single-cell single-extracellular vesicle (EV) analysis, individual cells, including tumor various types stromal EVs were segmented by multiclass pixelwise...
Abstract Biomarkers are indispensable for precision medicine. However, focused single-biomarker development using human tissue has been complicated by sample spatial heterogeneity. To address this challenge, we tested a representation of primary tumor that synergistically integrated multiple in situ biomarkers extracellular matrix from sampling regions into an intratumor graph neural network. Surprisingly, the differential prognostic value computational model over its conventional non-graph...
Abstract Label-free optical microscopy has matured as a noninvasive tool for biological imaging; yet, it is criticized its lack of specificity, slow acquisition and processing times, weak noisy signals that lead to inaccuracies in quantification. We introduce FOCALS (Fast Optical Coherence, Autofluorescence Lifetime imaging, Second harmonic generation) capable generating NAD(P)H fluorescence lifetime, second generation (SHG), polarization-sensitive coherence (OCM) images simultaneously....
Time-resolved photon counting methods have a finite bandwidth that restricts the acquisition speed of techniques like fluorescence lifetime imaging microscopy (FLIM). To enable faster imaging, computational can be employed to count photons when output detector is directly digitized at high sampling rate. Here, we present using hybrid photodetector in conjunction with multithreshold peak detection instances where one or more arrive within response time. This method used distinguish up five...
Stitched fluorescence microscope images inevitably exist in various types of stripes or artifacts caused by uncertain factors such as optical devices specimens, which severely affects the image quality and downstream quantitative analysis. Here, we present a deep learning-based Stripe Self-Correction method, so-called SSCOR. Specifically, propose proximity sampling scheme adversarial reciprocal self-training paradigm that enable SSCOR to utilize stripe-free patches sampled from stitched...
We report the numerical analysis of gradient index (GRIN) lens-based optical coherence tomography imaging probes to derive optimal design parameters. Long and short working distance with a small focal spot are considered. In each model, beam waist characterized compared for different values length refractive probe components. also explore influence outer tubing sample media. Numerical results show that adjustment maximum diameter focusing angle at end GRIN lens surface is very important...
Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid proliferation, cells not only alter fatty acid synthesis metabolism intracellularly extracellularly, but also macroenvironment via direct or indirect pathways. We report here, using Raman micro-spectroscopy, that an increase production polyunsaturated (PUFAs) was identified both cancerous normal appearing breast tissue obtained from patients tumor-bearing rats. By minimizing confounding...
Histochemistry is a microscopy-based technology widely used to visualize the molecular distribution in biological tissue. Recent developments label-free optical imaging has demonstrated potential replace conventional histochemical labels/markers (fluorescent antibodies, organic dyes, nucleic acid probes, and other contrast agents) with diverse interactions generate contrasts, allowing "virtual" histochemistry three spatial dimensions without preparing microscope slide (i.e. labor-intensive...