A5064056782- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Diabetes Management and Research
- Pancreatic function and diabetes
- Quasicrystal Structures and Properties
- Ionosphere and magnetosphere dynamics
- Pharmaceutical Practices and Patient Outcomes
- Diabetes and associated disorders
- Earthquake Detection and Analysis
- Diabetes Treatment and Management
- Surface Modification and Superhydrophobicity
- GNSS positioning and interference
- Probiotics and Fermented Foods
- Fluid Dynamics and Turbulent Flows
- Renal Diseases and Glomerulopathies
- Health Literacy and Information Accessibility
- RNA modifications and cancer
- Inorganic Chemistry and Materials
- Hormonal Regulation and Hypertension
- Pharmacy and Medical Practices
- Peptidase Inhibition and Analysis
- Photonic and Optical Devices
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
Kyoto University
2016-2025
Wakayama Medical University
2023-2025
Nara Medical University Hospital
1992-2025
Nara Medical University
2019-2024
Yokohama City University
1990-2024
Yokohama City University Medical Center
2018-2024
Osaka University of Pharmaceutical Sciences
2023
Shinshu University
2023
Jobu University
2023
Tsuchiura Kyodo General Hospital
2023
Background: Pharmacists, being one of the more readily accessible primary health care professionals, must ensure accessibility. With growing internationalization, those in Japanese community pharmacies increasingly interact with non-Japanese speakers. This study aimed to understand how can fulfill accessibility needs by accounting for patients’ native language and culture. Methods: A cross-sectional survey 2 municipalities (Hirakata Suma) was conducted between September October, 2022. The...
Lysine residues can be post-translationally modified by various acyl modifications in bacteria and eukarya. Here, we showed that two major modifications, acetylation succinylation, were changed response to the carbon source Gram-positive model bacterium Bacillus subtilis. Acetylation was more common when cells grown on glucose, glycerol, or pyruvate, whereas succinylation upregulated citrate, reflecting metabolic states preferentially produce acetyl-CoA succinyl-CoA, respectively. To...
Background: The relationship between the percent coefficient of variation (%CV) and risk severe hypoglycemia (SH) or non-severe (NSH) in patients with type 1 diabetes (T1D) remains to be elucidated. Materials Methods: Effect Intermittent-Scanning Continuous Glucose Monitoring Glycemic Control Including Hypoglycemia Quality Life Patients Type Diabetes Mellitus (ISCHIA) study was a crossover, randomized, controlled trial for prevention T1D using multiple daily injections (MDIs). Blinded...
Among the 57 genes that encode ribosomal proteins in genome of Bacillus subtilis, a Gram-positive bacterium, 50 were targeted by systematic inactivation. Individual deletion mutants 16 (L1, L9, L15, L22, L23, L28, L29, L32, L33.1, L33.2, L34, L35, L36, S6, S20, and S21) obtained successfully. In conjunction with previous reports, 22 have been shown to be nonessential B. at least for cell proliferation. Although several harbored protein gene did not show any significant differences phenotypes...
To elucidate the biological functions of small (p)ppGpp synthetases YjbM and YwaC Bacillus subtilis, we constructed RIK1059 RIK1066 strains carrying isopropyl-β-D-thiogalactopyranoside (IPTG) inducible yjbM ywaC genes, respectively, in ΔrelA ΔyjbM ΔywaC triple mutant background. While uninduced IPTG-induced cells grew similarly LB medium, growth was arrested following addition IPTG during early exponential phase. Induction expression by also severely decreased intracellular GTP level...
To elucidate the biological functions of ribosomal protein L34, which is encoded by rpmH gene, deletion mutant Bacillus subtilis and two suppressor mutants were characterized. Although ΔrpmH exhibited a severe slow-growth phenotype, additional mutations in yhdP or mgtE gene restored growth rate strain. Either disruption yhdP, thought to be involved efflux Mg(2+), overexpression mgtE, plays major role import could suppress defects both formation 70S ribosome caused absence L34. Interestingly,...
The number of copies rRNA (rrn) operons in a bacterial genome differs greatly among species. Here we examined the phenotypic effects variations genes Bacillus subtilis by analysis eight mutant strains constructed to carry from two nine rrn operon. We found that decrease ten one increased doubling time, and decreased sporulation frequency motility. maximum levels for transformation activity were similar strains, although competence development was significantly delayed strain with single...
In this study, we develop a three-dimensional ionospheric tomography with the ground-based global position system (GPS) total electron content observations. Because of geometric limitation GPS observation path, it is difficult to solve ill-posed inverse problem for density. Different from methods given by pervious studies, consider an algorithm combining least-square method constraint condition, in which gradient density tends be smooth horizontal direction and steep vicinity F2 peak. This...
Ribosome dimers are a translationally inactive form of ribosomes found in Escherichia coli and many other bacterial cells. In this study, we that the 70S Bacillus subtilis dimerized during early stationary phase these remained cytoplasm until regrowth was initiated. dimerization required hpf gene, which encodes homologue E. hibernation-promoting factor (Hpf). The expression induced at an its observed throughout rest experimental period, including entire 6 h phase. followed induction WT...
In Bacillus subtilis a null mutation of the relA gene, whose gene product is involved in synthesis and/or hydrolysis (p)ppGpp, causes growth defect that can be suppressed by mutation(s) yjbM ywaC coding for small (p)ppGpp synthetases. All 35 suppressor mutations newly isolated were classified into two groups, either or ywaC, mapping and sequencing their mutations, suggesting there are no synthetases other than RelA, YjbM, YwaC B. subtilis. order to understand better relation between RelA...
This paper reports on the development of a real-time three-dimensional ionospheric tomography technique using GNSS measurements. GPS measurement data from 200 receivers distributed throughout Japan are analyzed in real time to calculate Total Electron Contents (TECs). The electron density distribution was reconstructed slant TEC data. results were found be good agreement with both profiles obtained by radio occultation measurements COSMIC satellite and peak densities vertical sounding...
Abstract Bacillus subtilis differentiates into various cellular states in response to environmental changes. It exists two during the exponential growth phase: motile cells and connected chains of sessile cells. Here, we identified new regulators cell motility chaining, ribosomal proteins S21 (rpsU) S11 (rpsK). Their mutants showed impaired (observed a laboratory strain) robust biofilm formation an undomesticated strain). The major operons for formation, tapA-sipW-tasA epsA-O, were strongly...
Abstract Background Uterine leiomyomas are common for reproductive-aged women and affect women’s quality of life due to heavy menstrual bleeding or dysmenorrhea. Leiomyomas grow according estradiol exposure decrease after post-menopause. In case serious symptoms caused by leiomyomas, pharmacotherapy surgical treatment is proposed. Prior treatment, pharmacotherapies aimed at the reduction leiomyoma uterine volume improvement anemia introduced conduct minimum invasive surgery (i.e., reduce...
FreeStyle Libre has been approved for use in patients undergoing hemodialysis (HD) Japan, unlike Europe and the United States; however, evidence regarding its accuracy such is sparse. Forty-one participants with type 2 diabetes HD were recruited. The overall mean absolute relative difference 23.4% 33.9 mg/dL, respectively. Sensor glucose levels capillary significantly correlated (r = 0.858, P < .01), although sensor lower than levels. of became deteriorated days usage. percentage results...
The number of copies rRNA genes in bacterial genomes differs greatly among species. It is difficult to determine the functional significance heterogeneity each operon fully due existence multiple operons and because sequence extremely low. To overcome this problem, we sequentially deleted ten rrn Bacillus subtilis constructed seven mutant strains that harboured a single (either rrnA, B, D, E, I, J or O) their genome. growth rates sporulation frequencies these mutants were reduced drastically...
The case of a 68-year-old woman with purpura nephritis associated nephrotic syndrome is herein described. patient's clinical course and the findings renal biopsy study revealed nephritis. Following treatment corticosteroids intravenous cyclophosphamide accompanied by an angiotensin II type I receptor-blocker, anti-platelet drug hydroxymethylglutaryl (HMG)-CoA, proteinuria mildly decreased. Additional rituximab therapy led to complete remission. This report describes our successful experience...
Site-specific recombination (SSR) systems are employed for transfer of mobile genetic elements (MGEs), such as lysogenic phages and integrative conjugative (ICEs). SSR between attP/I attB sites is mediated by an integrase (Int) a directionality factor (RDF). The genome Bacillus subtilis 168 contains SPβ, active prophage, skin, defective ICEBs1, element. Each these MGEs harbors the classic unit attL-int-rdf-attR. Here, we demonstrate that units all compatible can substitute one another....
Abstract Single‐nucleotide polymorphisms (SNP) of ATP2B1 gene are associated with essential hypertension but their association resistant (RHT) remains unexplored. The authors examined the relationship between SNPs and RHT by genotyping 12 in 1124 Japanese individuals lifestyle‐related diseases. Patients had inadequate blood pressure (BP) control using three antihypertensive drugs or used ≥4 drugs. controlled BP ≤3 each SNP was analyzed logistic regression. final cohort 888 (79.0%) 43 (3.8%)...
Aim: Patients with orthostatic hypotension (OH) have high arterial stiffness. diabetes mellitus (DM) often cardiac autonomic neuropathy that leads to OH; however, whether OH is an indicator of stiffness progression unclear. We aimed investigate the cardio-ankle vascular index (CAVI) varies between DM patients and without using sit-to-stand test (STST).