A5101963413- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Mesenchymal stem cell research
- RNA regulation and disease
- Spinal Cord Injury Research
- Reproductive Biology and Fertility
- Botulinum Toxin and Related Neurological Disorders
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Reproductive Health and Technologies
- Pluripotent Stem Cells Research
- Viral Infections and Immunology Research
- Neurological diseases and metabolism
- Carbon and Quantum Dots Applications
- Graphene and Nanomaterials Applications
- Adipose Tissue and Metabolism
- Sperm and Testicular Function
- Reproductive System and Pregnancy
Asan Medical Center
2016-2025
Ulsan College
2011-2025
University of Ulsan
2013-2025
Neurological Surgery
2016-2023
Kyung Hee University
2022
Maria Fertility Hospital
2006
MizMedi Hospital
2001
BACKGROUND: The inhibition of neuronal activity by electrical deep brain stimulation is one the mechanisms explaining therapeutic effects in patients with Parkinson disease (PD) but cannot specifically activate or inactivate different types neurons. Recently, a new technology based on optogenetics has been developed to modulate specific However, optical inactivation subthalamic nucleus (STN) have not fully investigated. OBJECTIVE: To perform various behavioral tests evaluate changes motor...
Mutations in specific genes, including synuclein alpha (SNCA) that encodes the α-synuclein protein, are known to be risk factors for sporadic Parkinson's disease (PD), as well critical familial PD. In particular, A53T-mutated SNCA (A53T-SNCA) is a well-studied pathologic mutation However, techniques deletion of mutated gene vivo have not been developed. Here, we used CRISPR-Cas9 system delete A53T-SNCA vitro vivo. Adeno-associated virus carrying SaCas9-KKH with single-guide RNA targeting...
Monoamine oxidase-B (MAO-B) is a well-established therapeutic target for Parkinson's disease (PD); however, previous clinical studies on currently available irreversible MAO-B inhibitors have yielded disappointing neuroprotective effects. Here, we tested the potential of KDS2010, recently synthesized potent, selective, and reversible inhibitor in multiple animal models PD. We designed series α-aminoamide derivatives found that derivative KDS2010 exhibited highest potency, specificity,...
We investigated the neuroprotective effects of deca nano-graphene oxide (daNGO) against reactive oxygen species (ROS) and inflammation in human neuroblastoma cell line SH-SY5Y 6-hydroxydopamine (6-OHDA) induced Parkinsonian rat model. An MTT assay was performed to measure viability vitro presence 6-OHDA and/or daNGO. The intracellular ROS level quantified using 2',7'-dichlorofluorescein diacetate. daNGO showed 6-OHDA-induced toxicity also displayed scavenging properties. then tested...
In preclinical research of Parkinson's disease, several rodent models, notably the classical 6-hydroxydopamine (6-OHDA) model and A53T-alpha-synuclein model, have been widely used, yet their distinct neurochemical characteristics in conjunction with behavioral histopathological changes scarcely documented. We examined two rat models disease characterized them using [18F]FP-CIT animal PET imaging. The 6-OHDA (n=10) was induced by unilateral injection into middle forebrain bundle, while...
<b><i>Background:</i></b> The inhibition of neuronal activity by electrical deep brain stimulation is one the mechanisms explaining amelioration levodopa-induced dyskinesia. However, cannot specifically activate or inactivate selected types neurons. <b><i>Objectives:</i></b> We applied optogenetics as an alternative treatment to for dyskinesia, and also confirm that mechanism dyskinesia subthalamic nucleus mediated through inhibition....
Functional and neural tissue recovery has been reported in many animal studies conducted with stem cells. However, the combined effect of cytokines cells not yet adequately researched. Here, we analyzed additive effects granulocyte colony-stimulating factor (GCSF) on adipose-derived (ADSCs) infusion treatment acute spinal cord injury (SCI) rats.Four days after intrathecal tubes implantation Sprague-Dawley rats, SCI was induced an infinite horizon impactor. In Sham group (n=5),...
Transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy early-stage human dental papilla-derived and brain-derived in rat models 6-hydroxydopamine-induced Parkinson's disease. Rats received unilateral injection 6-hydroxydopamine into right medial forebrain bundle, followed 3 weeks later by injections PBS, cells, or ipsilateral striatum. All rats cell group died from tumor formation at...
Background: The classic animal model of Parkinson’s disease (PD) using neurotoxin can only simulate fixed stages the by causing irreversible damage to nigrostriatal system. Objectives: To develop an optogenetic PD that modulate severity optical stimulation introducing halorhodopsin (NpHR) gene into substantia nigra compacta. Methods: Fifteen rats received injections engineered AAV with NpHR-YFP nigra. They were then subjected illumination 590-nm light wavelengths 3 conditions, i.e.,...
Deep brain stimulation surgery has been performed in various movement disorders and psychiatric diseases. However, electrical may unexpectedly affect other types of adjacent neurons outside the target area. Recently, optogenetics provided opportunity to modulate specific neurons. Since this novel technique can individually control freely moving animals, it could be proposed restore neural circuit function related diseases, such as affective disorders, Huntington's disease (HD), Parkinson's...
Objective : Through our previous clinical trials, the demonstrated therapeutic effects of MSC in chronic spinal cord injury (SCI) were found to be not sufficient. Therefore, need develop stem cell agent with enhanced efficacy is increased. We transplanted Wnt3asecreting human mesenchymal cells (hMSC) into injured spines at 6 weeks after SCI improve axonal regeneration a rat model SCI. hypothesized that Wnt3a protein expression could augment neuro-regeneration SCI.Methods Thirty-six...
Abstract To date, no publicly available disease-modifying therapy for Parkinson’s disease has been developed. This can be partly attributed to the absence of techniques in vivo deletion SNCA gene (encoding α-synuclein), which is one key players pathology. In particular, A53T-mutated (A53T-SNCA) most studied familial pathologic mutations disease. Here we utilized a recently discovered genome editing technique, CRISPR/Cas9, delete A53T-SNCA vitro and . Among various CRISPR/Cas9 systems,...
Abstract Background and Purpose Monoamine oxidase-B (MAO-B) is a long-standing therapeutic target for Parkinson’s disease (PD), however, previous clinical studies demonstrated discouraging effects of currently available irreversible MAO-B inhibitors. Since KDS2010, novel, potent, selective, reversible inhibitor, has been developed, here we tested its potential in animal models PD. Experimental Approach We designed synthesized α-aminoamide derivatives compared the specificity to reversibility...
A double toxin-double lesion strategy is well-known to generate a rat model of striatonigral degeneration (SND) such as multiple system atrophy-parkinsonian type. However, with this it difficult distinguish SND from Parkinson's disease (PD). In study, we propose new SND, which generated by simultaneous injection 6-hydroxydopamine into the medial forebrain bundle and quinolinic acid striatum. Stepping tests performed 30 min after intraperitoneal L-dopa administration at 6 weeks post-surgery...
Introduction: Vitrification of human embryos is an efficient and simple method cryopreservation but it requires increased concentrations permeable cryoprotectants (40% v/v).However, none the studies analyze course pregnancies health infants born after vitrification.The MA-MA clinic practices vitrification since 2001.According to 2002-2005 statistics, total number births 34.This study undertakes summarize available data on labor vitrification.Materials methods: We use protocol conventionally...