A5100755476- Melanoma and MAPK Pathways
- Cancer-related molecular mechanisms research
- Mechanisms of cancer metastasis
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Dental materials and restorations
- NF-κB Signaling Pathways
- Dental Implant Techniques and Outcomes
- Genetic and phenotypic traits in livestock
- Musculoskeletal pain and rehabilitation
- Medical Imaging Techniques and Applications
- Adenosine and Purinergic Signaling
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- Lymphoma Diagnosis and Treatment
- Spine and Intervertebral Disc Pathology
- Glycosylation and Glycoproteins Research
- Autophagy in Disease and Therapy
- Dental Radiography and Imaging
Air Force Medical University
2022-2025
Xijing Hospital
2022-2025
Dalian Medical University
2013-2025
First Affiliated Hospital of Dalian Medical University
2011-2025
302 Military Hospital of China
2018-2025
Chinese PLA General Hospital
2020-2025
University of Michigan
2025
Chinese Academy of Agricultural Sciences
2013-2024
Ministry of Agriculture and Rural Affairs
2024
Institute of Animal Sciences
2013-2024
More than 60% of patients with hepatocellular carcinoma (HCC) do not receive curative therapy as a result late clinical presentation and diagnosis. We aimed to identify plasma microRNAs for diagnosing hepatitis B virus (HBV) -related HCC.Plasma microRNA expression was investigated three independent cohorts including 934 participants (healthy, chronic B, cirrhosis, HBV-related HCC), recruited between August 2008 June 2010. First, we used microarray screen 723 in 137 samples HCC. Quantitative...
Abstract Besides the conventional carbon sources, acetyl-CoA has recently been shown to be generated from acetate in various types of cancers, where it promotes lipid synthesis and tumour growth. The underlying mechanism, however, remains largely unknown. We find that induces a hyperacetylated state histone H3 hypoxic cells. Acetate predominately activates lipogenic genes ACACA FASN expression by increasing H3K9, H3K27 H3K56 acetylation levels at their promoter regions, thus enhancing de...
Increased fatty acid synthesis is required to meet the demand for membrane expansion of rapidly growing cells. ATP-citrate lyase (ACLY) upregulated or activated in several types cancer, and inhibition ACLY arrests proliferation cancer Here we show that acetylated at lysine residues 540, 546, 554 (3K). Acetylation these three stimulated by P300/calcium-binding protein (CBP)-associated factor (PCAF) acetyltransferase under high glucose increases stability blocking its ubiquitylation...
Diet-derived nutrients are inextricably linked to human physiology by providing energy and biosynthetic building blocks functioning as regulatory molecules. However, the mechanisms which circulating in body influence specific physiological processes remain largely unknown. Here we use a blood nutrient compound library-based screening approach demonstrate that dietary trans-vaccenic acid (TVA) directly promotes effector CD8
The spindle assembly checkpoint (SAC) is essential for proper sister chromatid segregation. Defects in this can lead to chromosome missegregation and aneuploidy. An increasing body of evidence suggests that aneuploidy play a causal role tumorigenesis. However, mutant mice are prone have only mild tumor phenotypes, suggesting there limiting factors the aneuploidy-induced Here we provide p53 such factor. We show activates loss drastically accelerates development two independent models....
Colonoscopy remains the standard screening method for detecting colorectal cancer (CRC) at an early stage. However, many people avoid having a colonoscopy because of fear its potential complications. Our study aimed to identify plasma microRNAs preliminarily CRC in general population, so that some unnecessary colonoscopies can be avoided. We investigated microRNA expression three independent cohorts including discovery (n = 80), training 112), and validation 49) phases recruited two medical...
Abstract Background Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, has been reported to exert its suppressive function via modulation expression in many malignancies, including breast cancer (BC). However, whether PTENP1/miR-20a/PTEN axis exists and how it functions BC progression remains elusive. Methods The levels miR-20a were measured by qRT-PCR. Furthermore, cells proliferation was further CCK8 assay, colony formation assays, EDU Ki67 staining. migratory invasive...
Circulating microRNAs are promising biomarkers for non‐invasive testing and dynamic monitoring in cancer patients. However, no consensus exists regarding the normalization of circulating quantification, making results incomparable. We investigated global microRNA profiles to identify a stable endogenous control quantifying using three cohorts ( n = 544), including 168 individuals (healthy subjects those with chronic hepatitis B cirrhosis) 376 patients (hepatocellular, colorectal, lung,...
We have previously demonstrated that nuclear factor kappa B (NFkappaB) activation is needed for the development of cardiac hypertrophy in vivo. NFkappaB a downstream transcription Toll-like receptor (TLR)-mediated signaling pathway; therefore, we investigated role TLR4 vivo.TLR4-deficient mice (C.C3H-Tlr4(lps-d), n = 6), wild-type (WT) genetic background (BALB/c, TLR4-deleted strain (C57BL/10ScCr, 8), and WT controls (C57BL/10ScSn, 8) were subjected to aortic banding 2 weeks. Age-matched...
The tumor suppressor p53 regulates cell cycle progression and apoptosis in response to various types of stress, whereas excess activity creates unwanted effects. Tight regulation is essential for maintaining normal growth. p53-associated cellular protein-testes derived (PACT, also known as P2P-R, RBBP6) a 250-kDa Ring finger-containing protein that can directly bind p53. PACT highly up-regulated esophageal cancer may be promising target immunotherapy. However, the physiological role PACT–p53...
To investigate the role of circKDM4C in acute myeloid leukemia (AML), expression circKDM4C, hsa-let-7b-5p, and P53 was measured by qRT-RCR. AML cell lines(K-562 HL-60) were transfected correspondingly investigated for proliferation, migration, invasion abilities CCK-8, colony formation, transwell, wound healing assays, respectively. The levels P53, ACSL4, PTGS2, GPX4, FTH1 K-562, HL-60 cells western blotting. Also, mediated regulation ferroptosis studied. Phen Green SK probe confocal laser...
Glutamate is a well-characterized excitatory neurotransmitter in the central nervous system (CNS). Recently, glutamate receptors (GluRs) were also found peripheral tissues, including heart. However, function of GluRs organs remains poorly understood. In present study, we that N-methyl-D-aspartate (NMDA) could increase intracellular calcium ([Ca(2+)]i) level dose-dependent manner cultured neonatal rat cardiomyocytes. NMDA at 10(-4) M increased levels reactive oxygen species (ROS), cytosolic...
We hypothesized that atherosclerosis inhibits NO-mediated endothelium-dependent dilation of coronary arterioles through interaction ox-LDL with its receptor, LOX-1, the production O2ÿ- in endothelial cells.We assessed role dysfunction a murine model (ApoE KO mice). Coronary from WT control and ApoE mice were isolated pressurized without flow. Although vessels to endothelium-independent vasodilator SNP was not altered between mice, agonist, ACh reduced versus mice. Impaired vasodilation is...
Endothelium-derived hyperpolarizing factor (EDHF) plays a crucial role in modulating vasomotor tone, especially microvessels when nitric oxide-dependent control is compromised such as diabetes. Epoxyeicosatrienoic acids (EETs), potassium ions (K+), and hydrogen peroxide (H2O2) are proposed EDHFs. However, the identity (or identities) of EDHF-dependent endothelial dilators has not been clearly elucidated We assessed mechanisms EDHF-induced vasodilation wild-type (WT, normal), db/db (advanced...
Cardiac microvascular endothelial cells (CMECs) dysfunction contributes to cardiovascular complications in diabetes, whereas, the underlying mechanism is not fully clarified. FoxO transcription factors are involved apoptosis and reactive oxygen species (ROS) production. Therefore, present study was designed elucidate potential role of FoxO3a on CMECs injury induced by high glucose.CMECs were isolated from hearts adult rats cultured normal or glucose medium for 6 h, 12 h 24 respectively. To...
Many studies demonstrated unique microRNA profiles in lung cancer. Nonetheless, the role and related signal pathways of miR-375 cancer are largely unknown. Our study investigated relationships between carcinogenesis adenocarcinoma, squamous cell carcinoma small to identify new molecular targets for treatment. We evaluated 723 microRNAs microdissected cancerous cells adjacent normal from 126 snap-frozen specimens using microarrays. validated expression its 22 putative target mRNAs an...
Immunotherapy has been successfully used in the treatment of multiple malignancies, but clinical studies revealed low response rates. Thus, development new effective immunotherapeutic modalities is urgently needed. Successfully inducing tumor cell death with enhanced antigenicity important for expansion and differentiation tumor-specific CD8+ cytotoxic T lymphocytes. Cationic liposome/DNA complexes (CLN/DNA), which usually have obvious effects, may improve antitumor immunity through...
The increased coronavirus disease 2019 (COVID-19) breakthrough cases pose the need of booster vaccination. We conducted a randomised, double-blinded, controlled, phase 2 trial to assess immunogenicity and safety heterologous prime-boost vaccination with an inactivated COVID-19 vaccine (BBIBP-CorV) followed by recombinant protein-based (NVSI-06-07), using homologous boost BBIBP-CorV as control. Three groups healthy adults (600 individuals per group) who had completed two-dose vaccinations 1-3...
Addressing the challenge of promoting directional axonal regeneration in a hostile astrocytic scar, which often impedes recovery following spinal cord injury (SCI), remains daunting task. Cell transplantation is promising strategy to facilitate nerve restoration SCI. In this research, pro-regeneration system developed, namely miR-26a@SPIONs-OECs, for olfactory ensheathing cells (OECs), preferred choice SCI patients. These entities show high responsiveness external magnetic fields (MF),...
Calcium homeostasis is critical for cell proliferation, and emerging evidence shows that cancer cells exhibit altered calcium signals to fulfill their need proliferation. However, it remains unclear whether there are oncogene-specific regulations can expose novel therapeutic targets. Here, from RNAi screen, we report adenosylhomocysteinase like protein 1 (AHCYL1), a suppressor of the endoplasmic reticulum (ER) channel inositol trisphosphate receptor (IP3R), selectively upregulated...