A5037909273- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Pediatric Hepatobiliary Diseases and Treatments
- Gallbladder and Bile Duct Disorders
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
- Nanoplatforms for cancer theranostics
- Liver Disease Diagnosis and Treatment
- Cell death mechanisms and regulation
- Sarcoma Diagnosis and Treatment
- Heat shock proteins research
- Drug Transport and Resistance Mechanisms
- Physiological and biochemical adaptations
- Inflammatory mediators and NSAID effects
- Hepatocellular Carcinoma Treatment and Prognosis
- TGF-β signaling in diseases
- Pancreatitis Pathology and Treatment
Shandong University
2015-2024
Shandong Provincial Hospital
2015-2024
Shandong First Medical University
2022-2024
Shangdong Agriculture and Engineering University
2016
National Natural Science Foundation of China
2016
Jiangsu Province Hospital
2004
Accumulating evidence has shown that up-regulation of microRNA-25(miR-25) is associated with the prognosis several types human malignant solid tumors. However, whether miR-25 expression influence on hepatocellular carcinoma (HCC) still unknown. The differentially expressed amount was validated in triplicate by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Survival rate analyzed log-rank test, and survival curves were plotted according to Kaplan–Meier. Multivariate...
The aim of this manuscript is to analyze the diagnostic and prognostic value circulating miR-18a in plasma patients with gastric cancer. In study, 82 cancer 65 healthy controls were enrolled 10 ml peripheral venous blood was collected for RNA extraction. expression determined using TaqMan quantitative real-time polymerase chain reaction assay further correlated patients’ clinicopathological parameters follow-up data. results indicated that upregulated compared (P < 0.001). yielded an area...
Abstract Background Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory linc01134 hepatocellular carcinoma (HCC) has not been studied yet. Materials and methods qRT-PCR western blot were conducted measure relevant RNA protein expressions. CCK-8, colony formation, EdU, flow cytometry, wound-healing, transwell assays xenograft experiments performed determine HCC. ChIP luciferase reporter analyze...
This study aimed to investigate the expression, biological function, and downstream mechanism of LINC00511 in gastric cancer (GC). In paired GC samples, LINC00511, miR-625-5p STAT3 mRNA expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR); protein was immunohistochemical (IHC). The gain-of-function loss-of-function models established, proliferative migrative ability cells measured CCK-8 transwell assays, respectively. regulatory relationship between...
Context: Icaritin (ICT), a prenylflavonoid derivative extracted from the Epimedium (Berberidaceae) genus, has been identified to exhibit antitumor effect in hepatocellular carcinoma (HCC) cells by inducing apoptosis. However, its on cellular senescence not elucidated. Objective: To investigate mechanism for low concentrations of ICT exerting activity through induction senescence. Materials and methods: Human HepG2 Huh7 were treated with (1 2 μM) once per day week. Cellular was evaluated cell...
Abstract LncRNA TP73 antisense RNA 1T (TP73-AS1) plays an important role in human malignancies. However, the levels of TP73-AS1 and its functional mechanisms pancreatic cancer metastasis remain unknown, clinical significance is also unclear. In present study, candidate target miR-141 adjacent normal tissue were detected using qRT-PCR. The association between clinicopathologic characteristics patients analyzed. relationship miR-141, 3-hydroxybutyrate dehydrogenase type 2 (BDH2) was confirmed...
Hepatocellular carcinoma (HCC) is a highly mortal cancer that could be treated by radiotherapy. DNA damage response (DDR) vital factor affecting development after Long non-coding RNAs (lncRNAs) have been revealed to regulate and repair in cells. Nevertheless, the function of long intergenic non-protein coding RNA 1134 (LINC01134) has not explored DDR. In this study, we targeted digging into LINC01134 DDR exploring underlying mechanism HCC RT-qPCR was employed measure expression, found...
Tumor necrosis factor (TNF)-alpha-induced protein 8 (TNFAIP8 or TIPE) is a recently identified considered to be associated with carcinogenesis. To investigate its expression pattern in pancreatic cancer patients and analyse correlation clinicopathological significance the levels of epithelial growth receptor (EGFR), immunohistochemistry was performed detect TNFAIP8 EGFR proteins cancers, pancreatitis tissues, healthy controls. The results showed stronger staining tissues compared normal...
Previous studies have shown that the expression level of stanniocalcin 2 (STC2) is associated with tumor progression. However, to date, association between STC2 and clinicopathological factors in hepatocellular carcinoma (HCC) has not been investigated. The clinical significance was investigated 30 fresh HCC samples using western blot analysis 240 tissues immunohistochemical analysis. cancerous tissue higher than matched non-cancerous tissues. Using immunohistochemistry, STC2-positive group...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies with broad resistance to chemotherapeutic drugs. Krüppel-like factor 4 (KLF4) a candidate tumor suppressor in PDAC. However, precise role KLF4 gemcitabine PDAC remains largely unclear. In this study, we demonstrated that inhibited expression. Moreover, also reduced levels miR‑200b and miR‑183, but promoted ZEB1 expression cells. knockdown blocked inversely, overexpression suggesting positively regulated miR‑183....
Though nanomedicine-based photothermal therapy (PTT) has demonstrated promising prospect in tumor treatment due to its high therapeutic efficiency and controllable range, the overexpression of heat shock proteins (HSPs) during PTT can lead intracellular thermal resistance reduce effectiveness. Reactive oxygen species (ROS), followed by application chemodynamic (CDT) photodynamic (PDT), eliminate HSPs overcome resistance. However, microenvironment, including hypoxia glutathione (GSH)...
To further delineate the clinicopathological and radiological features of solid pseudopapillary tumor (SPT) pancreas summarize surgical therapy strategy for this tumor.A retrospective review 18 pathologically confirmed cases SPT was performed clinical pathological features, findings interventions were analyzed.The patients included 17 females 1 male with a median age 23 years. The diameter lesions 8.0 cm. Abdominal pain predominant complaint (8/18). rest asymptomatic presented pancreatic...
Previous studies have focused on the changes of tumour cells in immune escape, and less is known about effect microenvironment (TME) escape. Tumour-associated fibroblasts (TAF) an important part TME has special physiological biochemical characteristics, but specific mechanism not been clarified. In order to investigate TAF expression PD-L1 gastric cancer cells, cell lines MNK45, SGC7901 were non-contact co-culturing with 1, 3 7 d via transwell. mRNA protein detected using qRT-PCR FCM. Then,...
The prognosis of hepatocellular carcinoma (HCC) treated by radiofrequency ablation (RFA) is mainly associated with tumor recurrence. So far, no tissue biomarker recurrence has been confirmed in biopsy specimens. Previous studies have reported that aberrant expression microRNA-34a (miR-34a) involved oncogenesis and progression HCC. aim this study was to investigate the prognostic value miR-34a patients HCC RFA. Patients early-stage single-nodule RFA were included, assessed quantitative...
To evaluate the clinical characteristics and radiological features of solid pseudopapillary tumor (SPT) assess surgical therapy strategy.A retrospective review was performed in 62 patients pathologically confirmed SPT treated between 2003 2014. The features, examinations strategies were analyzed.56 females 6 males included this study, mean age 26 years old (range: 8-66 old) with size 7.2 cm 3-15 cm), most commonly located head pancreas (n = 29). Among all cases, 3 had liver metastasis...
Abstract Dysregulation of microRNAs (miRNAs) expression has been demonstrated in gastrointestinal stromal tumor (GIST). In this study, we aimed to determine the differential miRNAs GISTs and explore functional mechanism GIST cells. We measured six pairs tissues matched adjacent through a high‐throughput sequencing, which was confirmed 64 by real‐time polymerase chain reaction. found that miR‐4510 significantly downregulated compared control tissues. Luciferase reporter assay identified...
G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number biological and pathological processes, have recently emerged as key players carcinogenesis cancer progression. Orphan G protein-coupled (oGPCRs) are group proteins lacking endogenous ligands. GPR137, one novel oGPCR genes, was discovered by homology screening. However, role GPR137 cancers has not yet been discussed is great therapeutic interest. In this study, we knocked down via lentivirus...
Cellular senescence, a state of irreversible growth arrest, occurs in all somatic cells and causes the to exhaust replicative capacity. Recently, cellular senescence has been emerging as one principal mechanisms tumor suppression, which can be induced by low doses therapeutic drugs cancer cells. Acetyl-11-keto-β-boswellic acid (AKBA), an active ingredient isolated from plant Boswellia serrata, identified induce apoptosis hepatocellular carcinoma (HCC) In this study, we found that...
Colorectal cancer (CRC) is the third most commonly diagnosed and second highest cause of cancer‑associated death worldwide. Talin1 activates integrins, which mediate cell adhesion, proliferation, tumorigenesis metastasis. The aim present study was to determine talin1 expression levels in colorectal investigate role CRC proliferation invasion vitro vivo. protein were detected human adjacent normal tissues by immunohistochemistry. short hairpin RNA control vectors designed stably transfected...
Objective Myonectin, a newly discovered myokine, enhances fatty acid uptake in cultured adipocytes and hepatocytes suppresses circulating levels of free acids mice. Recent studies showed that serum myonectin concentration is negatively correlated with obesity. This study was undertaken to evaluate the change obese patients after laparoscopic sleeve gastrectomy. Methods performed population 42 58 control subjects from April 2018 December 2019. All underwent Anthropometric measurements, lipid...