A5009377592- Nitric Oxide and Endothelin Effects
- Diabetes Treatment and Management
- Eicosanoids and Hypertension Pharmacology
- Nuclear reactor physics and engineering
- Liver Disease and Transplantation
- Nuclear Materials and Properties
- Liver Disease Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Alcohol Consumption and Health Effects
- Metabolism, Diabetes, and Cancer
- Graphite, nuclear technology, radiation studies
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Urinary Bladder and Prostate Research
- Fusion materials and technologies
- Diamond and Carbon-based Materials Research
- Metal and Thin Film Mechanics
- Force Microscopy Techniques and Applications
- Parathyroid Disorders and Treatments
- Hematopoietic Stem Cell Transplantation
- Organ Transplantation Techniques and Outcomes
- Nuclear and radioactivity studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Neuroscience of respiration and sleep
- Thyroid and Parathyroid Surgery
Tokyo Medical University Hospital
2015-2021
Japan Atomic Energy Agency
2011-2021
Riso Kagaku (Japan)
2013-2020
Tokyo Medical University
2019
National Institute of Technology, Ube College
2006-2008
Tohoku University
2008
Choju Medical Institute
2008
Toyota Technological Institute
1999-2005
Teikyo University
2004-2005
Kawasaki Hospital
2005
Hepatic microcirculatory perturbation is observed after ischemia/reperfusion. Endothelin-1, a potent vasoconstrictive peptide, known to modulate local circulation. This study was designed examine whether endothelin-1 participates in the mechanism of disturbance and damage liver Ischemia median left lateral lobes induced for 60 min; it followed by reperfusion 24 hr. In some rats, antiserum or control serum without endothelin-1-blocking activity administered intravenously just before...
Background & AimsCD26, a multifunctional transmembrane glycoprotein, is expressed in various cancers and functions as dipeptidyl peptidase 4 (DPP4). We investigated whether CD26 expression associated with hepatocellular carcinoma (HCC) progression DPP4 inhibitors exert antitumor effects against HCC.MethodsCD26 was examined 41 surgically resected HCC specimens. The of on were by using cell lines (Huh-7 Li-7), xenograft tumors nude mice, nonalcoholic steatohepatitis–related mouse...
Growing attention has been focused on the roles of proximal tubules (PTs) kidney in glucose metabolism, including mechanism regulation gluconeogenesis. In this study, we found that PT-specific insulin receptor substrate 1/2 double-knockout mice, established by using newly generated sodium-glucose cotransporter 2 (SGLT2)-Cre transgenic exhibited impaired signaling and upregulated gluconeogenic gene expression renal gluconeogenesis, resulting systemic resistance. contrast,...
Nonalcoholic steatohepatitis (NASH) is a hepatic phenotype of the metabolic syndrome, and increases risk cirrhosis hepatocellular carcinoma (HCC). Although increasing evidence points to therapeutic implications certain types anti-diabetic agents in NASH, it remains be elucidated whether their effects on NASH are independent diabetes. Genetically obese melanocortin 4 receptor-deficient (MC4R-KO) mice fed Western diet murine model that sequentially develops steatosis, HCC presence obesity...
This study was designed to investigate the mechanism for ethanol-induced hepatic vasoconstriction in isolated perfused rat liver. Upon initiation of ethanol infusion into portal vein at concentrations ranging from 25 100 mM, pressure began increase a concentration-dependent manner and reached maximal levels 2-5 min (initial phase), followed by gradual decrease over period (escape phenomenon). Endothelin-1 antiserum significantly inhibited this 45-80%. Cessation endothelin-1 subsequent...
The purpose of this study was to evaluate the role endothelin-1 in modulating hepatic microcirculation and liver damage. Rats were infused with at doses ranging from 30 1,000 pmol/kg over 1 min through an indwelling cannula placed portal vein. In control rats, saline solution same rate. Alterations measured vivo microscopy system. Serum lactate dehydrogenase activity, indicator damage, hr after infusion. Immediately infusion endothelin-1, we noted a rapid increase pressure, which remained...
We examined whether endogenous inhibitors of NO synthesis are involved in the augmentation intimal hyperplasia rabbits with hyperglycaemia induced by alloxan. Four weeks after endothelial denudation carotid artery which had been performed 12 alloxan, was greatly augmented hyperglycaemia. The degree assessed using three different parameters histopathological findings as well changes luminal area and intima : media ratio. There were positive significant correlations between ratio, plasma...
This study examines whether tumor necrosis factor and endotoxin are involved in the pathogenesis of primary nonfunction graft pulmonary complication after orthotopic liver transplantation. Livers from Lewis rats were stored for either 1 or 4 hr ice-cold Euro-Collins solution (1-hr storage 4-hr group, respectively). Subsequently, donor livers implanted orthotopically. In some experiments, anti-tumor antibody was administered intravenously before immediately surgery into animals that received...
Dipeptidyl peptidase-4 inhibitors are used for treatment of patients with type 2 diabetes. In addition to glycemic control, these agents showed beneficial effects on lipid metabolism in clinical trials. However, the mechanism underlying lipid-lowering effect dipeptidyl remains unclear. Here, we investigated efficacy anagliptin a hyperlipidemic animal model, and examined action.Male low-density lipoprotein receptor-deficient mice were administered 0.3% their diet. Plasma levels assayed...
The purpose of this study was to determine whether hepatic tissue oxygenation after ischemia-reperfusion procedures is an indicator for later liver injury. Partial ischemia in the induced by ligating left pedicles. Rats were divided into two groups according duration ischemia: group A (30-min ischemia) and B (60-min ischemia). Indices blood volume regional tissue, serum ALT levels histological appearance livers evaluated. Twenty-four hours reflow, all rats alive, whereas only 67% survived B....
We examined whether endogenous inhibitors of nitric oxide (NO) synthesis are involved in the impaired cavernosal relaxation with ischemia rabbits. Two weeks after caused by partial vessel occlusion, endothelium-dependent and electrical field stimulation (EFS)-induced neurogenic NO-mediated relaxations, but not sodium nitroprusside (SNP)-induced relaxation, were significantly isolated corpus cavernosum. The Ca 2+ -dependent NO synthase (NOS) activity basal stimulated cGMP productions...
The role of microcirculation in the pathogenesis alcoholic liver injury was investigated isolated perfused livers from fed rats. Infusion ethanol into portal vein at concentrations ranging 25 to 200 mmol/L increased pressure, which is an indicator hepatic vasoconstriction, a concentration-dependent fashion. Portal pressure started rise immediately on initiation load and remained higher than basal levels throughout period infusion. Release lactate dehydrogenase, cell injury, effluent...
The purpose of this study was to clarify the role endogenous nitric oxide in ethanol-induced perturbation microcirculation and hepatic injury perfused rat liver. Infusion ethanol into portal vein at 25 100 mmol/L increased pressure, which is an indicator vasoconstriction, a concentration-dependent fashion. Portal pressure started rise immediately after load, then decreased gradually remained higher than basal levels throughout period infusion. Release lactate dehydrogenase effluent perfusate...
Long-term storage of liver grafts results in increased adhesion leukocytes onto the sinusoidal walls. This eventually leads to posttransplant graft damage through disturbances hepatic microcirculation. Intracellular molecule-1 (ICAM-1) is known be involved attachment leukocytes. study was designed examine whether ICAM-1 participated pathogenesis injury. Inbred Lewis rats were used as both donors and recipients avoid immunoreactivity. Donor livers stored for either 1 or 6 hr ice-cold...
Several studies have demonstrated that ethanol can increase hepatic O2 uptake (e.g., produce a hypermetabolic state); however, complete explanation of this important phenomenon remains unclear. Here, the effect conditioned media from Kupffer cells isolated rats chronically exposed to on consumption normal parenchymal was studied evaluate possibility cell-cell communication participates in mechanism state. were fed either liquid control diet or containing ethanol. cultured for 4 h, and...
The human calcium-sensing receptor (CaSR) is a G protein-coupled that maintains extracellular Ca2+ homeostasis by regulating the secretion of parathyroid hormone. Upacicalcet novel positive allosteric modulator CaSR used for treatment secondary hyperparathyroidism. In present study, to clarify binding site upacicalcet CaSR, we conducted studies and agonistic activity in HEK-293T cells expressing (intact mutant) an silico docking-simulation analysis. As result, competed with L-tryptophan was...
1. We examined the effect of ischaemia on neurogenic and nitric oxide (NO)-mediated urethral relaxation. 2. Rabbits were divided into control (UI) groups, which was prepared by partial occlusion bilateral iliac arteries using blood vessel occluders. 3. Neurogenic NO-mediated proximal relaxation induced electrical field stimulation (EFS) greatly impaired in UI group, while sodium nitroprusside (SNP) as a NO donor showed no difference between two groups. Pretreatment with L-arginine...
Calcium is essential for activation of Kupffer cells and it was recently reported that contain L-type voltage-dependent Ca++ channels. The purpose the present study to evaluate effect chronic ethanol treatment on were isolated from rats fed either a control liquid diet or ethanol-containing 5 8 weeks. cytosolic free calcium concentration ([Ca++]i) measured in freshly with fluorescent indicator, fura-2. In group, partial replacement extracellular Na+ by K+ caused concentration-dependent...