Pathology in the primary drainage pathway for aqueous humor eye is responsible ocular hypertension, only treatable risk factor patients with glaucoma. Unfortunately, mechanisms that regulate pressure-dependent of and thus intraocular pressure (IOP) are unknown. To better understand one possible underlying molecular regulates IOP, nitric oxide (NO), transgenic mice overexpressing endothelial NO synthase (eNOS) was studied.IOP measured by rebound tonometry mice, versus flow data were ex vivo...

A novel layered double hydroxide (LDH) nanoparticle/thermogel composite drug delivery system (DDS) for sustained release of brimonidine (Bri) has been designed, prepared, and characterized in this study treatment severe glaucoma. Brimonidine is first loaded onto LDH (Bri@LDH) nanoparticles, which are then dispersed the thermogel consisting plenty micelles based on poly(dl-lactic acid-co-coglycolic acid)-polyethylene glycol-poly(dl-lactic acid) (PLGA-PEG-PLGA) copolymer. The Bri@LDH/Thermogel...

Abstract Background Particulate matter (PM) is strongly linked to human health and has detrimental effects on the eye. Studies have, however, focused ocular surface, with limited research impact of PM 2.5 intraocular pressure (IOP). Methods To investigate IOP associated mechanism, C57BL/6 mouse eyes were topically exposed a suspension for 3 months, trabecular meshwork (HTM) cells subjected various concentrations in vitro. Cell viability, NLRP3/caspase-1, IL-1β, GSDMD expression, reactive...

Although studies have demonstrated that fine particulate matter (PM2.5) induces ocular surface damage, PM2.5 exposure causes cornea toxicity is not entirely clear. The aim of this study to investigate the role nod-like receptor family pyrin domain containing three (NLRP3) inflammasome-mediated pyroptosis in PM2.5-related corneal toxicity. Human epithelial cells (HCECs) were exposed different concentrations PM2.5, and cell viability, expressions NLRP3 inflammasome mediated axis molecules...

OBJECTIVE Postoperative neurological deficits are a significant concern for surgeons during spinal deformity correction surgery. Preoperative identification of high-risk patients can help ensure that appropriate measures taken to minimize the risk these procedures. Previous studies have shown with large Cobb angles or angular ratios (DARs) at higher postoperative deficits. However, some curves similar and DARs may exhibit significantly different risks Existing methods evaluating degree...

Mice are commonly used in glaucoma research, but relatively little is known about aqueous outflow dynamics the species. To facilitate future use of mouse as a model humor outflow, several fundamental physiological parameters were measured eye.

Abstract Nitric oxide (NO) donors are ideal drug candidates for reducing intraocular pressure in the treatment of glaucoma. However, poor cornea penetration, short duration efficacy, and narrow therapeutic index most NO obstruct their clinical applications glaucoma treatment. This study reports a novel donor delivery system based on mesoporous silica nanoparticles that can readily overcome above difficulties deliver NO‐donating sodium nitroprusside to target tissues (trabecular meshwork...

Retrospective study.To investigate the incidence, risk factors, and outcomes of pedicle screw loosening in degenerative lumbar scoliosis (DLS) undergoing long-segment spinal fusion surgery.One hundred thirty DLS patients who underwent surgery with at least a 12-month follow-up were studied. The incidence factors investigated. VAS, SRS-22, ODI scores obtained preoperatively follow-up.One sixty-eight 1784 (9.4%) screws showed evidence 71 (54.6%) patients. Three required revision surgery. Screw...

Glaucoma is characterized by high intraocular pressure (IOP), oxidative stress, and distinct optic nerve damage. Natural enzymes have unparalleled advantages in the treatment of glaucoma due to their efficiency, specificity, selectivity. However, poor stability recoverability constrained application. The selection good immobilization carriers an effective strategy protect natural enzymes. Here, we employ a mild room-temperature aqueous-phase enzyme technique immobilize superoxide dismutase...

Purpose: To investigate the role of endothelial nitric oxide synthase (eNOS) on conventional outflow function using NOS3 knockout (KO) mice. Methods: Intraocular pressure was measured in both KO and wild type (WT) by rebound tonometry. Outflow facility perfusing enucleated mouse eyes at multiple steps. A subset sectioned stained for histology. Mock aqueous humor ± (NO) donors nitroprusside dihydrate (SNP) or S-Nitroso-N-Acetyl-D,L-Penicillamine (SNAP) perfused into eyes. SNP SNAP...

To investigate endothelial nitric oxide synthase (eNOS) activity and the response of conventional outflow facility to donors a (NOS) inhibitor in caveolin-1 (CAV1) knockout (KO) mice.Intraocular pressure (IOP) was measured both CAV1 KO wild-type (WT) mice by rebound tonometry. The expressions caveolin-2 (CAV2), eNOS, eNOS-phospho Ser1177, Thr495, Akt, Akt-phospho Ser473, nitrotyrosin were Western blot analysis. Nitric donor sodium nitroprusside (SNP), S-nitroso-N-acetyl-D,L-penicillamine...

The cornea provides important protection for human eyes from invasion of alien substances. However, its blockage on the infiltration molecules also constitutes a great challenge noninvasive trans-cornea delivery drugs. Here we report polyamino acid-based S-nitrosothiols with high cationic charge density as NO carrier to overcome associated in ophthalmological therapy. Our results demonstrate that nature polymer promoted transcytosis, which greatly enhances donor and bypasses barriers passive...

Filtration surgery is currently being acknowledged as the most effective therapeutic modality for glaucoma; however, excessive scarring of a filtering bleb often leads to surgical failure. Subconjunctival administration drug delivery systems provides localized and sustained antifibrotic drugs inhibit post-operative scarring. In this study, long-acting system Cyclosporine A (CsA) was developed using thermogelling poly-(dl-lactic acid-co-glycolic acid)-poly(ethylene glycol)-poly-(dl-lactic...

Polydopamine–polyethylenimine nanoparticles (PDA/PEI NPs) have been successfully applied as miRNA carriers, and PDA/PEI NPs/miR-21-5p plays a role in increasing the aqueous humor drainage lowering intraocular pressure.

Ambient air pollution is related to the onset and progression of ocular disease. However, effect pollutants on acute glaucoma remains unclear.To investigate incidence (acute angle closure glaucomatocyclitic crisis) among adults.We conducted a time-stratified case-crossover study based data outpatients from January, 2015 Dec, 2021 in Shanghai, China. A conditional logistic regression model combined with polynomial distributed lag was applied for statistical analysis. Each case serves as its...