A5019406362- Congenital Heart Disease Studies
- Preterm Birth and Chorioamnionitis
- Advanced Neuroimaging Techniques and Applications
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- MRI in cancer diagnosis
- Proteoglycans and glycosaminoglycans research
- Advanced X-ray and CT Imaging
- Radiation Dose and Imaging
- Connective tissue disorders research
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Pulmonary Hypertension Research and Treatments
- Uterine Myomas and Treatments
- Advanced MRI Techniques and Applications
- Gestational Diabetes Research and Management
- Intracranial Aneurysms: Treatment and Complications
- Cardiac Imaging and Diagnostics
- Ectopic Pregnancy Diagnosis and Management
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pediatric Hepatobiliary Diseases and Treatments
- Cervical Cancer and HPV Research
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Sperm and Testicular Function
Tokyo University of the Arts
2024
The University of Tokyo
2024
Nagoya City University
2013-2023
Osaka University
2013-2022
Mizuho (Japan)
2012-2021
Nagoya City University Hospital
2016
Tokyo Women's Medical University Hospital
2013
Akita University
1998-2011
Akita University Hospital
2010-2011
Seikagaku Corporation (Japan)
2000
The recombinant clustered regulatory interspaced short palindromic repeats ( CRISPR )/Cas system has opened a new era for mammalian genome editing. Here, we constructed pX330 plasmids expressing humanized Cas9 (hCas9) and single guide RNA s (sg s) against mouse genes validated them both in vitro vivo . When randomly chose 291 target sequences within protein coding regions of 73 genes, an average number off‐target candidates (exact match 13 nucleotides from 3′ NGG) found by Bowtie software...
About 10% of male infertile patients show abnormalities in spermatogenesis. The microdeletion azoospermia factor a (AZFa) region the Y chromosome is thought to be cause spermatogenic failure. However, candidate gene responsible for failure AZFa deleted has not been elucidated yet. Using mice, we explored function Ddx3y, strong Azfa region, and Ddx3x, Ddx3y paralog on X chromosome, We first generated KO mice using CRISPR/Cas9 found that normal spermatogenesis, produce morphologically...
Acrosin, the trypsin-like serine protease in sperm acrosome, was long viewed as a key enzyme required for zona pellucida penetration to fertilize eggs. However, gene disruption experiments mice surprisingly showed that acrosin-disrupted males were fertile. Thus, acrosin considered be not an essential fertilization mice. involvement of has been suggested various species such rat, bull, and pig. Moreover, it reported (including acrosin) activity is significantly weaker compared other species,...
Fertilization occurs as the culmination of multi-step complex processes. First, mammalian spermatozoa undergo acrosome reaction to become fusion-competent. Then, acrosome-reacted penetrate zona pellucida and adhere finally fuse with egg plasma membrane. IZUMO1 is first sperm protein proven be essential for sperm-egg fusion in mammals, Izumo1 knockout mouse but fail oolemma. However, function other species remains largely unknown. Here, we generated rats by CRISPR/Cas9 found male were...
Various magnetic resonance imaging (MRI) techniques including T2*-weighted imaging, susceptibility-weighted and MR relaxometry had been performed to evaluate different patterns of brain iron depositions in Parkinsonian syndrome. The aim the present study was diagnostic value a volume interest (VOI) analysis on principles echo shifting with train observations (PRESTO) using statistical parametric mapping (SPM) 8 WFU PickAtlas program for diagnosis syndrome.,Methods: Fifty subjects, 13 variant...
Background.Chorioamnionitis is considered to be one of the important causes preterm labor.To investigate relationship between inflammatory cytokines in amniotic fluid and histologic evidence chorioamnionitis, we studied interleukin-6 (IL-6) levels patients with labor.Methods.Between 1993 1996, obtained by amniocentesis from 110 before 32 weeks gestation who had labor on admission.We measured IL-6 an ELISA method.Histologic examination placentae fetal membranes after delivery was evaluated.As...
In vertebrate development, ectoderm is specified into neural plate (NP), border (NPB), and epidermis. Although such patterning thought to be achieved by molecular concentration gradients, it has been revealed, mainly in vitro analysis, that mechanical force can regulate cell specification. During vivo patterning, cells deform migrate, this applies surrounding tissues, shaping the embryo. However, role of for specification largely unknown. study, with an aspiration assay atomic microscopy, we...
We report a 64-year-old man with incidentally found uncorrected total anomalous pulmonary venous connection (TAPVC). There have been only few case reports of untreated TAPVC diagnosed after 60 years age. Also, this is first in which ECG-gated CT and phase-contrast cine magnetic resonance imaging (PC-MRI) was performed. He referred to our hospital for the surgery rectal cancer. had an arterial septal defect (ASD) persistent left superior vena cava (PLSVC), Eisenmenger's syndrome thought be...
The purpose of this study was to examine differences in texture features between olfactory neuroblastoma (ONB) and sinonasal squamous cell carcinoma (SCC) on contrast-enhanced CT (CECT) images, evaluate the predictive accuracy analysis compared radiologists' interpretations. Forty-three patients with pathologically-diagnosed primary nasal paranasal tumor (17 ONB 26 SCC) were included. We extracted 42 from regions CECT images obtained before treatment. In univariate analysis, each compared,...
Reported herein is a case of hydrops fetalis in which the cord blood expressed cytomegalovirus (CMV) antigen. Fetal ascites was removed from an infected fetus with utero and 2.5 g CMV hyperimmunoglobulin administered into fetal abdominal cavity at 28 weeks gestation. After immunoglobulin administration, vanished, preload index inferior vena cava decreased platelet count infant increased. However, despite intrauterine therapy intensive neonatal care, died soon after birth. The expression...
Blister-like aneurysm of the supraclinoid internal carotid artery (ICA) is a well-documented cause subarachnoid hemorrhage. Generally, this type associated with various conditions such as hypertension, arteriosclerosis, and ICA dissection. Although Aspergillus most common organism causing intracranial fungal aneurysmal formation, there no report blister-like caused by infection.An 83-year-old man received corticosteroid pulse therapy followed oral steroid for an inflammatory pseudotumor...
Abstract Aim: To evaluate surgical indications for combined partial rectosigmoidectomy in ovarian cancer with direct invasion of the rectum and sigmoid colon or dissemination into pouch Douglas. Methods: Subjects comprised 25 patients who underwent primary surgery between 1990 2002 at our hospital. Federation Obstetrics Gynecology staging tumors was II ( n = 6), III 17) IV 2). The histologic type serous adenocarcinoma 18), clear cell 4), others 3). Bowel resection performed during 18...