A5066671819- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Adenosine and Purinergic Signaling
- GDF15 and Related Biomarkers
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- Effects of Radiation Exposure
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Genetics and Neurodevelopmental Disorders
- Gut microbiota and health
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer, Lipids, and Metabolism
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- interferon and immune responses
- Telomeres, Telomerase, and Senescence
- Genomics, phytochemicals, and oxidative stress
- bioluminescence and chemiluminescence research
- Colorectal Cancer Surgical Treatments
- Radiation Effects and Dosimetry
- Ultrasound and Hyperthermia Applications
Sun Yat-sen University
2017-2024
Sixth Affiliated Hospital of Sun Yat-sen University
2017-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2018
University of Pennsylvania
2004-2010
University of Louisville
2010
Children's Hospital of Philadelphia
2004
Laboratoire de Chimie
1998-2000
Centre National de la Recherche Scientifique
1999
Université de Strasbourg
1999
Abstract Gastric cancer (GC), one of the most common malignant tumors in world, exhibits a rapid metastasis rate and causes high mortality. Diagnostic markers potential therapeutic targets for GCs are urgently needed. Here we show that Actin-like protein 6 A (ACTL6A), encoding an SWI/SNF subunit, is highly expressed GCs. ACTL6A found to be critical regulating glutathione (GSH) metabolism pathway because it upregulates γ-glutamyl-cysteine ligase catalytic subunit (GCLC) expression, thereby...
Background Several nonviral vectors including linear polyethylenimine(L-PEI) confer a pronounced lung tropism to plasmid DNA when injected into the mouse tail vein in nonionic solution. Methods and results We have optimized this route by injecting 50 µg with excess L-PEI (PEI nitrogen/DNA phosphate=10) large volume of 5% glucose (0.4 ml). In these conditions, 1–5% cells were transfected (corresponding 2 ng luciferase/mg protein), other organs remaining essentially refractory transfection...
Background The ideal non-viral vector should be cell-type directed and form complexes with DNA that are physically stable, small electrically neutral. Methods We have synthesized several PEI derivatives coat the PEI/DNA water-soluble residues able to stabilize particles, mask their surface charge eventually direct them a particular tissue. morphologies sizes of were observed by TEM DLS techniques, apparent was quantitated zeta potential measurements; in vitro transfection efficacies...
Colon cancer is one of the most common cancers with high mortality in humans. Early diagnosis and treatment colon great significance for therapy. Numerous theranostic agents have been developed to detect kill cells. However, few reports focused on how these control affect gene expression cells vivo. Herein, three pyridinium-functionalized tetraphenylethylene derivatives, namely, TPE-OM, TPE-H, TPE-NO2, electron-donating electron-withdrawing groups were facilely synthesized as cell imaging...
Abstract Background NOP2/Sun domain 2 (NSUN2) is one of the important RNA methyltransferases catalyzing 5‐methylcytosine (m5C) formation and participates in many critical bioprocesses. However, roles underlying molecular mechanisms NSUN2‐mediated m5C modification colorectal cancer (CRC) remain unclear. Methods To explore NSUN2 expression CRC, fresh tissue samples were collected Nsun2 knockout mouse was constructed. In vitro vivo functional assays conducted to assess role NSUN2. array...
Abstract Background Colorectal cancer (CRC) is the leading worldwide. Microbial agents have been considered to contribute pathogenesis of different disease. But underlying relevance between CRC and microbiota remain unclear. Methods We dissected fecal microbiome structure genomic transcriptomic profiles matched tumor normal mucosa tissues from 41 patients. Of which, relationship CRC-associated bacterial taxa their significantly correlated somatic mutated gene was investigated by exome...
Abstract The Serine–Glycine–One-Carbon (SGOC) pathway is pivotal in multiple anabolic processes. Expression levels of SGOC genes are deregulated under tumorigenic conditions, suggesting participation oncogenes deregulating the biosynthetic pathway. However, underlying mechanism remains elusive. Here, we identified that Interleukin enhancer-binding factor 3 (ILF3) overexpressed primary CRC patient specimens and correlates with poor prognosis. ILF3 critical regulating by directly mRNA...
Abstract Metabolic reprogramming can contribute to colorectal cancer progression and therapy resistance. Identification of key regulators metabolism could provide new approaches improve treatment reduce recurrence. Here, we demonstrate a critical role for the COP9 signalosome subunit CSN6 in rewiring nucleotide cancer. Transcriptomic analysis patient samples revealed correlation between expression purine pyrimidine metabolism. A colitis-associated model established that Csn6 intestinal...
Abstract Background Cancer stem cells (CSCs) are responsible for tumour initiation, metastasis and recurrence. However, the mechanism of CSC formation, maintenance expansion in colorectal cancer (CRC) remains poorly characterised. Methods The role COP9 signalosome subunit 6 (CSN6) regulating stemness was evaluated by organoid formation limited dilution analysis. CSN6–TRIM21–OCT1–ALDH1A1 axis vitro vivo. association CSN6, TRIM21 ALDH1A1 expression validated a tissue microarray with 267 CRC...
Abstract Forkhead‐Box Class O 4 (FOXO4) is involved in critical biological functions, but its response to EGF‐PKB/Akt signal regulation not well characterized. Here, it reported that FOXO4 levels are downregulated EGF treatment, with concurrent elevation of COP9 Signalosome subunit 6 (CSN6) and E3 ubiquitin ligase constitutive photomorphogenic 1 (COP1) levels. Mechanistic studies show CSN6 binds regulates stability through enhancing the activity COP1, COP1 directly interacts a VP motif on...
We, and others, have observed that the structure of cationic lipids appears to a significant effect on transfection efficacy optimized nucleic acid/cationic lipid complexes (lipoplexes) used for in vitro vivo gene delivery expression. Although there are many comparisons reagents delivery, few been made vivo. We previously reported effects changes hydrophobic domain chain length asymmetry, headgroup composition, counterion exchange. our own work over years apparent superiority asymmetric...
Platelet-derived growth factor C signaling promotes radiation proctopathy development and can be inhibited in a mouse model.
An understanding of the time course and correlation with injury heat shock proteins (HSPs) released during brain and/or spinal cord cellular stress (ischemia) is critical in role HSPs survival, may provide a clinically useful biomarker severe stress. We have analyzed levels cerebrospinal fluid (CSF) from patients who are undergoing thoracic aneurysm repair. Blood CSF samples were collected at regular intervals, was by enzyme-linked immunosorbent assay for HSP70 HSP27. These results...
Chemoresistance limits treatment outcomes in colorectal cancer (CRC) patients. A dimeric metabolite of indole-3-carbinol, 3,3'-diindolylmethane (DIM) is abundant cruciferous vegetables and has shown anticancer efficacy. The role DIM regulating chemosensitivity CRC remains unknown. In this study, we demonstrated that inhibits the malignant progression CRC. RNA sequencing indicated pyrimidine synthesis genes are attenuated by treatment. Stable 13C-labeled glucose tracing revealed de novo...
We previously showed that a vector:lipid delivery system, comprised of plasmid DNA vector and cationic lipid (lipoplex), when injected into the cerebrospinal fluid (CSF) rats can deliver reporter genes in vivo efficiently with widespread expression to Central Nervous System (CNS). To further characterize this we now present experiments demonstrate time-to-peak gene, firefly luciferase. infused formulated lipoplex containing MLRI [dissymmetric myristoyl (14:0) lauroyl (12:1) rosenthal...
Abstract BAF53A, an important subunit of the SWI/SNF epigenetic chromatin regulatory complex, has been implicated as driver diverse cancers. However, role BAF53A in colorectal cancer (CRC) remains poorly understood. Here, we examined expression CRC samples and observed that was significantly upregulated tissues compared with paired adjacent normal tissues. In vitro vivo studies suggested ectopic promoted cell proliferation, colony formation, tumorigenesis, whereas knockdown hindered these...
Rho GDP ‐dissociation inhibitor α (Rho GDI α) is an essential regulator for GTP ases. Although may serve as oncogene in colorectal cancer ( CRC ), the underlying mechanism still unclear. We investigated function, mechanism, and clinical significance of progression. founded that downregulation repressed cell proliferation, motility, invasion. Overexpression increased DNA damage response signals at telomeres, led to telomere shortening cells, also being validated 26 pairs tissues. Mechanistic...
Attachment of targeted ligands to nonviral DNA or RNA delivery systems is a promising strategy that seeks overcome the poor target selectivity generally observed in systemic applications. Several methods have been developed for conjugation lipids polymers, however, direct onto lipid- polymer-nucleic acid complexes not as straightforward. Here, we examine an oximation approach directly label lipoplex formulation. Specifically, report synthesis cationic diketo lipid DMDK, and its use...
Background The ideal non-viral vector should be cell-type directed and form complexes with DNA that are physically stable, small electrically neutral. Methods We have synthesized several PEI derivatives coat the PEI/DNA water-soluble residues able to stabilize particles, mask their surface charge eventually direct them a particular tissue. morphologies sizes of were observed by TEM DLS techniques, apparent was quantitated zeta potential measurements; in vitro transfection efficacies...