A5064109407- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Microfluidic and Bio-sensing Technologies
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- Blood properties and coagulation
- Innovative Microfluidic and Catalytic Techniques Innovation
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Cancer Research and Treatments
- Biosensors and Analytical Detection
- Plant Virus Research Studies
- Membrane Separation Technologies
- Nosocomial Infections in ICU
Fraunhofer Institute for Toxicology and Experimental Medicine
2021-2024
Technische Universität Braunschweig
2024
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
2019-2022
The aim of this study was to gain further insight into the diversity Escherichia coli phagesfollowed by enhanced work on taxonomic issues in that field. Therefore, we present genomiccharacterization and classification 50 bacteriophages against E. isolated fromvarious sources, such as manure or sewage. All phages were examined for their host range a setof different strains, originating, e.g., from human diagnostic laboratories poultry farms.Transmission electron microscopy revealed...
Bacteriophage therapy holds promise in addressing the antibiotic-resistance crisis, globally and Germany. Here, we provide an overview of current situation (2023) applied phage supporting research The authors, interdisciplinary group working on patient-focused bacteriophage research, addressed production, banks, susceptibility testing, clinical application, ongoing translational regulatory situation, network structure They identified critical shortcomings including lack trials, a paucity...
Worldwide, poultry industry suffers from infections caused by avian pathogenic Escherichia coli. Therapeutic failure due to resistant bacteria is of increasing concern and poses a threat human animal health. This causes high demand find alternatives fight bacterial in farming. Bacteriophages are being especially considered for the control multi-drug their specificity lack serious side effects. Therefore, study aimed on characterizing phages composing phage cocktail suitable prevention with...
Phage therapy of ventilator-associated pneumonia (VAP) is great interest due to the rising incidence multidrug-resistant bacterial pathogens. However, natural or therapy-induced immunity against therapeutic phages remains a potential concern. In this study, we investigated innate and adaptive immune responses two different phage cocktails targeting either Pseudomonas aeruginosa Escherichia coli—two VAP-associated pathogens—in naïve mice without confounding effects infection. Active...
Respiratory infections caused by multidrug-resistant
Among intestinal coliform microbes in the broiler gut, there are potentially pathogenic Escherichia (E.) coli that can cause avian colibacillosis. The treatment with antibiotics favors selection of multidrug-resistant bacteria and an alternative to this is urgently required. A chicken model colonization apathogenic strain E. was used test if oral phage application prevent or reduce gut extraintestinal variants two individual experiments. E28 as a strain, which could be differentiated from...
Due to the overuse of antibiotics, number multidrug-resistant pathogen bacteria is rising in recent years posing a serious threat human health. One promising alternative for treatment application phage therapy using highly selective bacteriophages. Because their selectivity, individual screens called phagograms each patient are required select phages from library. Phagograms mostly performed via bacterial cultivation on double layer agar plates and addition causing lysis. However, these...
Nosocomial infections contribute to 10-20% of ICU mortality, depending on the ventilation status, making ventilator-associated pneumonia (VAP) most common fatal hospital-acquired infection. Common bacterial lung pathogens include multidrug-resistant (MDR) bacteria, like carbapenem-resistant P. aeruginosa, K. pneumoniae, A. baumannii and E. coli, which are listed as "critical priority" bacteria by WHO. Bacteriophage (phage) therapy could possibly be an attractive option for VAP therapy. In a...
Abstract Combined use of virulent bacteriophages (phages) and antibiotics reduces the severity difficult-to-treat Pseudomonas aeruginosa infections in many patients. In vitro methods that attempt to reproduce more than one physiological state pathogens can provide a valuable assessment antibacterials like phages. Here, by measuring bacterial killing kinetics individual replication different growth conditions, including biofilms human lung epithelial cell line, we elucidated factors...
<title>Abstract</title> Bacterial multidrug resistance poses an urgent challenge for the treatment of critically ill patients developing ventilator-associated pneumonia (VAP). Phage therapy, a potential alternative when conventional antibiotics fail, has been unsuccessful in first clinical trials used alone. Whether combining with phages may enhance effectiveness remains to be tested experimental models. Here, we murine model <italic>Pseudomonas aeruginosa</italic>-induced VAP compare...
Introduction: Antimicrobial resistance constitutes one of the major public health concerns. Pseudomonas aeruginosa (P. aeruginosa) causes mainly respiratory infections and is most prevalent multidrug resistant bacteria. Bacteriophages are viruses, which natural predators bacteria highly specific for their host strain without affecting other bacterial species. However, local application preferred to ensure contact with cells. Nebulization a very effective way transport pharmaceuticals into...
<b>Introduction:</b> In 2014, the WHO proclaimed beginning of a post-antibiotic era [1]. As alternatives to antibiotics, bacteriophages are increasingly coming into focus combat increasing number resistant bacteria. order reach infected sites in lungs and reduce unwanted systemic effects, nebulization drugs is favorable way application. The infectivity phages with different tail lengths after was tested under various conditions. This project part Phage4Cure, BMBF-funded study examining use...
Introduction Patients with chronic lung diseases, such as COPD, bronchiectasis or cystic fibrosis, frequently suffer from airway colonization Pseudomonas aeruginosa. Recurrent exacerbations are associated high morbidity and mortality. The management of these patients is complicated by increasing number multi-drug resistant strains, creating a need for new therapeutic approaches. Bacteria-specific viruses (bacteriophages) have regained interest agents in recent years, although sufficient data...