A5029339397- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cardiac Fibrosis and Remodeling
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Asthma and respiratory diseases
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- FOXO transcription factor regulation
- Atherosclerosis and Cardiovascular Diseases
- Nutrition, Genetics, and Disease
- Carcinogens and Genotoxicity Assessment
- Adipokines, Inflammation, and Metabolic Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neuroinflammation and Neurodegeneration Mechanisms
- Delphi Technique in Research
- Single-cell and spatial transcriptomics
- Cardiac Valve Diseases and Treatments
- Cystic Fibrosis Research Advances
Boston University
2016-2025
University School
2025
Intarcia Therapeutics (United States)
2020
Boston Medical Center
2018
Veracyte (United States)
2017
University of California, Los Angeles
2017
University of Minnesota
2012
VA Tennessee Valley Healthcare System
2011
HudsonAlpha Institute for Biotechnology
2011
Vanderbilt-Ingram Cancer Center
2011
Breast cancer progression is associated with aberrant DNA methylation and expression of genes that control the epithelial-mesenchymal transition (EMT), a critical step in malignant conversion. Although affected have been studied, there little understanding how activation machinery itself occurs. Using breast cell-based model system, we found cells underwent EMT exhibited overactive transforming growth factor beta (TGFbeta) signaling loss CDH1, CGN, CLDN4, KLK10 as result hypermethylation...
Rationale: Molecular phenotyping of chronic obstructive pulmonary disease (COPD) has been impeded in part by the difficulty obtaining lung tissue samples from individuals with impaired function.Objectives: We sought to determine whether COPD-associated processes are reflected gene expression profiles bronchial airway epithelial cells obtained bronchoscopy.Methods: Gene profiling brushings 238 current and former smokers without COPD was performed using Affymetrix Human 1.0 ST...
Abstract Cigarette smoke creates a molecular field of injury in epithelial cells that line the respiratory tract. We hypothesized transcriptome sequencing (RNA-Seq) will enhance our understanding response to tobacco exposure and lung cancer pathogenesis by identifying gene expression differences not interrogated or accurately measured microarrays. sequenced high-molecular-weight fraction total RNA (>200 nt) from pooled bronchial airway cell brushings (n = 3 patients per pool) obtained...
Chronic obstructive pulmonary disease (COPD) is a heterogeneous consisting of emphysema, small airway obstruction, and/or chronic bronchitis that results in significant loss lung function over time. In order to gain insights into the molecular pathways underlying progression emphysema and explore computational strategies for identifying COPD therapeutics, we profiled gene expression tissue samples obtained from regions within same with varying amounts emphysematous destruction smokers (8 × 8...
Abstract In brain, the striatum is a heterogenous region involved in reward and goal-directed behaviors. Striatal dysfunction linked to psychiatric disorders, including opioid use disorder (OUD). subregions are divided based on neuroanatomy, each with unique roles OUD. OUD, dorsal altered processing, formation of habits, development negative affect during withdrawal. Using single nuclei RNA-sequencing, we identified both canonical (e.g., dopamine receptor subtype) less abundant cell...
Previous studies have shown that physiological responses to cigarette smoke can be detected via bronchial airway epithelium gene expression profiling and heterogeneity in this response smoking is associated with lung cancer. In study, we sought determine the similarity of effects tobacco throughout respiratory tract by determining patterns smoking-related paired nasal epithelial brushings collected from 14 healthy nonsmokers 13 current smokers. Using whole genome arrays, identified 119 genes...
<h3>Background</h3> A core feature of chronic obstructive pulmonary disease (COPD) is the accelerated decline in forced expiratory volume one second (FEV<sub>1</sub>). The recent Groningen and Leiden Universities study Corticosteroids Obstructive Lung Disease (GLUCOLD) suggested that particular phenotypes COPD benefit from fluticasone±salmeterol by reducing rate FEV<sub>1</sub> decline, yet underlying mechanisms are unknown. <h3>Methods</h3> Whole-genome gene expression profiling using...
Background: We previously derived and validated a bronchial epithelial gene expression biomarker to detect lung cancer in current former smokers. Given that nasal are similarly altered by cigarette smoke exposure, we sought determine if cancer-associated might also be detectable the more readily accessible epithelium. Methods: Nasal brushings were prospectively collected from smokers undergoing diagnostic evaluation for pulmonary lesions suspicious AEGIS-1 (n = 375) AEGIS-2 130) clinical...
There are 160,000 cancer patients worldwide treated with particle radiotherapy (RT). With the advent of proton, and high (H) charge (Z) energy (E) HZE ionizing RT, cardiovascular diseases risk estimates uncertain. In addition, future deep space exploratory-type missions will expose humans to unknown but low doses irradiation (IR). We examined molecular responses using transcriptome profiling in left ventricular murine cardiomyocytes isolated from mice that were exposed 90 cGy, 1 GeV proton...
Ribosomal RNA (rRNA) comprises at least 90% of total extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment mature poly-adenylated transcripts targeted depletion the rRNA fraction. The latter method is particular interest because it compatible with degraded samples such as those FFPE and also captures that are not some non-coding RNAs. Here we provide a cross-site study evaluates...
This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information.Multiple recent publications on RNA sequencing (RNA-seq) have demonstrated the power of next-generation technologies in whole-transcriptome analysis. Vendor-specific protocols used for library construction often require at least 100 ng total RNA. However, under certain conditions, much less is available construction. In these cases, effective transcriptome profiling requires amplification...
In chronic peripheral inflammation, endothelia in brain capillary beds could play a role for the apolipoprotein E4 (ApoE4)-mediated risk Alzheimer's disease (AD) risk. Using human tissues, here we demonstrate that interactions of endothelial CD31 with monomeric C-reactive protein (mCRP) versus ApoE were linked shortened neurovasculature AD pathology and cognition. knock-in mice, discovered intraperitoneal injection mCRP, via binding to on surface increased phosphorylation (pCD31), leading...
Abstract Objectives Examination of bronchial epithelium-derived gene expression signature bronchiectasis (BE) in nasal epithelium. Methods We studied 220 participants from the Detection Early Lung Cancer Among Military Personnel study with bulk RNA-seq epithelium brushings. Gene set enrichment analysis (GSEA) was used to examine whether genes previously identified as increased or decreased individuals radiologic BE are significantly enriched among most altered GSEA and cell-type specific...
Infiltrating macrophages contribute to muscle dystrophic changes in Duchenne muscular dystrophy (DMD). In a DMD mouse model, mdx 5cv mice, CC chemokine receptor type 2 (CCR2) deficiency diminishes Ly6C hi macrophage infiltration by blocking blood inflammatory monocyte recruitment. This is accompanied transient improvement of damage, fibrosis, and regeneration. The benefit, however, lost after the expansion intramuscular lo macrophages. To address mechanisms underlying expansion, we compared...
Reactive oxygen and nitrogen radicals produced during metabolic processes, such as respiration inflammation, combine with DNA to form many lesions primarily at guanine sites. Understanding the roles of polymerases responsible for processing these products mutations could illuminate molecular mechanisms that correlate oxidative stress cancer. Using M13 viral genomes engineered contain single Escherichia coli strains specific polymerase (pol) knockouts, we show pol V is required efficient...
Basal-like breast cancer (BLBC) is an aggressive subtype of which often enriched with stem cells (CSC), but the underlying molecular basis for this connection remains elusive. We hypothesized that BLBC are able to establish a niche permissive maintenance CSCs and found tumor cell-derived periostin (POSTN), component extracellular matrix, as well corresponding cognate receptor, integrin α(v)β(3), highly expressed in subset cell lines CSC-enriched populations. Furthermore, we demonstrated...
Small RNA sequencing can be used to gain an unprecedented amount of detail into the microRNA transcriptome. The relatively high cost and low throughput bases technologies potentially offset by use multiplexing. However, multiplexing involves a trade-off between increased number sequenced samples reduced reads per sample (i.e., lower depth coverage). To assess effect different depths owing on differential expression detection, we small lung tissue collected in clinical setting one, three,...
Sickle cell disease is a growing health burden afflicting millions around the world. Clinical observation and laboratory studies have shown that severity of sickle ameliorated in individuals who elevated levels fetal hemoglobin. Additional pharmacologic agents to induce sufficient hemoglobin diminish clinical an unmet medical need. We recently found up-regulation peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) can synthesis human primary erythroblasts. Here, we report...
Aflatoxin B 1 (AFB ), the most potent member of aflatoxin family hepatocarcinogens, upon metabolic activation reacts with DNA and forms a population covalent adducts. The prevalent adduct, 8,9-dihydro-8-(N 7 -guanyl-)-9-hydroxyaflatoxin -N -dG), as well AFB formamidopyrimidine adduct -FAPY), resulting from imidazole ring opening major are thought to be responsible for mutations caused by . -dG lesions rapidly removed in Escherichia coli mammals, whereas -FAPY persist mammalian cells, which...
The aim was to investigate whether microRNA (miRNA) expression is modulated by inhaled corticosteroid (ICS) treatmentWe performed genome-wide miRNA analysis on bronchial biopsies of 69 moderate/severe chronic obstructive pulmonary disease (COPD) patients at baseline and after 6- 30-month treatment with the ICS fluticasone propionate or placebo. effect validated in differentiated primary epithelial cultures, functional studies were conducted BEAS-2B cells. MiRNAs affected their predicted...
// Deqiang Huang 1 , Xiaoling He 2, 3 Junrong Zou Pei Guo Shanshan Jiang Nonghua Lv Yuriy Alekseyev 4 Lingyu Luo Zhijun 3, 5 Research Institute of Digestive Diseases, The First Affiliated Hospital Nanchang University, Nanchang, Jiangxi, China 2 Graduate Program, Jiangxi Medical College, P.R. Basic Sciences, Departments Pathology and Laboratory Medicine, Boston University School Boston, MA 02118, USA Department Biochemistry, Correspondence to: Luo, e-mail: zluo@bu.edu 15270855639@163.com...
Understanding effects of acute smoke exposure (ASE) on airway epithelial gene expression and their relationship with the chronic may provide biological insights into development smoking-related respiratory diseases.Bronchial cell brushings were collected from 63 individuals without recent cigarette before 24 h after smoking three cigarettes. RNA these samples was profiled Affymetrix Human Gene 1.0 ST microarrays.We identified 91 genes differentially expressed ASE (false discovery rate <...