A5101682175- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Surface Roughness and Optical Measurements
- Plant Molecular Biology Research
- Entrepreneurship Studies and Influences
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Traditional Chinese Medicine Studies
- Ion channel regulation and function
- Vasculitis and related conditions
- Industrial Vision Systems and Defect Detection
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV-related health complications and treatments
- Extracellular vesicles in disease
- Medical Research and Treatments
- Regional Economic and Spatial Analysis
- Mycobacterium research and diagnosis
- Educational Reforms and Innovations
- Light effects on plants
- 3D Printing in Biomedical Research
- HIV Research and Treatment
- Biochemical and Structural Characterization
Cell Technology (China)
2024
Suizhou Central Hospital
2022-2024
National Medical Products Administration
2019-2024
Huazhong University of Science and Technology
2008-2023
Xi'an Jiaotong University
2022-2023
Union Hospital
2021-2023
Shenzhen University Health Science Center
2021
State Key Laboratory of Modern Optical Instruments
2019
ShenZhen People’s Hospital
2019
Guangdong Medical College
2019
6-Phosphogluconate dehydrogenase (6PGD) is a key enzyme that converts 6-phosphogluconate into ribulose-5-phosphate with NADP+ as cofactor in the pentose phosphate pathway (PPP). 6PGD commonly upregulated and plays important roles many human cancers, while mechanism underlying such of remains elusive. Here we show upon EGFR activation, phosphorylated at tyrosine (Y) 481 by Src family kinase Fyn. This phosphorylation enhances activity increasing its binding affinity to therefore activates PPP...
Recapitulating the complex structural, mechanical, and electrophysiological properties of native myocardium is crucial to engineering functional cardiac tissues. Here, we report a leaf-venation-directed strategy that enables compaction remodeling cell-hydrogel hybrids into highly aligned densely packed organizations in predetermined patterns. This contributes interconnected tubular structures with cell alignment along hierarchical channels. Compared randomly-distributed cells, engineered...
The efficacy of immunotherapy based on natural killer (NK) cells is hampered by intrinsic non-specific cytotoxicity and insufficient activation NK cells. Here, we confer the T-cell receptor-like (TCR-like) specificity cells, taking advantage both innate adaptive immune arms response to generate enhanced anti-melanoma activity. TCR-like antibody (Ab) GPA7 was selected against melanoma-associated gp100/human leukocyte antigen (HLA)-A2 complex then fused intracellular domain CD3-ζ chain. This...
Low temperature could significantly induce anthocyanin accumulation in the presence of light. Recently, two bZIP transcription factors LONG HYPOCOTYL 5 (HY5) and HOMOLOG OF HY5 (HYH) were identified to play an important role process low temperature-induced accumulation. However, mechanism by which HY5/HYH regulates under still remains unclear. Here, we found that gibberellins (GAs) decrease but PAC (endogenous GAs biosynthesis inhibitor) increase accumulation, implying signaling may involve...
Antibodies (Abs) have been engineered into small antigen‐binding fragments and rebuilt multivalent high‐avidity molecules for improving in vivo pharmacokinetics efficacy clinical use. To increase the avidity of a T‐cell receptor‐like single‐domain Ab (sdAb) specific HLA‐A2 complex, we fused sdAb to coiled‐coil peptide derived from human cartilage oligomeric matrix protein (COMP48) make an multimer, termed combody. The combody improved binding significantly, whereas specificity targeted cells...
Acute myocardial infarction (AMI) is a fatal cardiovascular disease with high mortality rate. The discovery of effective biomarkers crucial for the diagnosis and treatment AMI. In present study, miRNA sequencing reverse transcription-quantitative polymerase chain reaction techniques revealed that expression exosome derived miR-152-5p was significantly downregulated in patients AMI compared healthy controls. A series functional validation experiments were then performed using H9c2...
The detection data of IgM and IgG antibodies in 169 patients with coronavirus disease-2019 (COVID-19) were analyzed to evaluate differences clinical performance between the colloidal gold method chemiluminescence method. In this study, antibody showed a positive conversion earlier (about 1-2 days earlier), rates higher different stages disease, trend declining rate later than For antibody, climbing more quickly No obvious negative-converting tendency was observed within 35 after onset...
AbstractDNA vaccine has become an attractive approach for generating antigen-specific immunity. Targeting antigens to FcRs IgG (Fc_Rs) on dendritic cells (DCs) been demonstrated enhance antigen presentation. Secondary lymphoid tissue chemokine (SLC) shown increase immune responses not only by promoting co-clustering of T and DCs in the lymph nodes spleen but also regulating their immunogenic potential induction cell responses. In this study, using HPV 16 E7 as a model antigen, we constructed...
The prevention and prognosis of the onset or recurrence acute myocardial infarction (AMI) is a difficult problem in contemporary research.In this study, peripheral blood samples were collected from seven patients with AMI nine healthy adults, exosome microRNAs (miRNAs) extracted. miRNA differential expression profiles serum exosomes obtained by using next-generation sequencing technology combined bioinformatics analysis. Reverse-transcriptase polymerase chain reaction (RT-PCR) was used to...
Although coronavirus disease 2019 (COVID-19) remains a global threat, administering effective and safe vaccines is currently the most promising strategy to curb ongoing pandemic decrease number of severe acute respiratory syndrome 2 (SARS-CoV-2) infections. However, there some uncertainty regarding safety for patients with kidney disease.A 58-year-old man presented at our institution gross hematuria 48 hours after receiving his first dose CoronaVac (Sinovac) vaccine.Analysis renal biopsy...
Natural killer (NK) cells play a key role in innate immunity and are regarded as promising candidate for cellular immunotherapy. may be generated from different sources, including induced pluripotent stem (iPSCs); these produce an abundant amount of NK to meet the needs wide range clinical applications. Autologous iPSCs expensive labor-intensive prepare, while allogeneic require human leukocyte antigen (HLA) matched avoid risk immune rejection. In current study, we prepared HLA-matched using...
The purpose of this study was to investigate the role legumain in formation and stability atherosclerotic plaque, as well explore association between with Smad3 pathway a rat atherosclerosis model. Rat thoracic aorta established received treatment statin (n = 15 each) or controls 10). Serum level determined by enzyme-linked immunosorbent assay. Legumain aortic expression levels were assessed immunohistochemistry fluorescence microscopy. Protein mRNA analyzed using Western blot analysis...
Drugs are very important for human life because they can provide treatment, cure, prevention, or diagnosis of different diseases. However, also cause side effects, which increase the risks humans and pharmaceuticals companies. It is essential to identify drug effects in discovery. To date, lots computational methods have been proposed predict drugs most them used fact that similar always effects. previous studies did not analyze substructures highly related kind effect.In this study, we...
From the perspective of performance evaluation, concerns range pathogens, establishment enterprise reference material, reaction system study and analytical performances evaluation central nervous infection pathogen metagenome sequencing reagent are briefly described, including methods quality control requirements. This is intended to increase research development efficiency products, contribute associated industry.