A5106466904- Telomeres, Telomerase, and Senescence
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Protist diversity and phylogeny
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Nanoparticles: synthesis and applications
- Microbial Community Ecology and Physiology
- Renal and related cancers
- Mitochondrial Function and Pathology
- Cancer Research and Treatments
- Cancer-related gene regulation
- Aquatic Ecosystems and Phytoplankton Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Kruppel-like factors research
- Genetics and Neurodevelopmental Disorders
- Chemical Reactions and Isotopes
- Advanced Nanomaterials in Catalysis
- Chromatin Remodeling and Cancer
- Cancer, Hypoxia, and Metabolism
- Hedgehog Signaling Pathway Studies
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- Bacteriophages and microbial interactions
- Genetic factors in colorectal cancer
University of Science and Technology Beijing
2021-2024
Duke Medical Center
2010-2021
Duke University Hospital
2009-2021
Duke University
2003-2020
Occupational Cancer Research Centre
2014
Pediatric Brain Tumor Foundation
2009-2013
Howard Hughes Medical Institute
2003
Sidney Kimmel Comprehensive Cancer Center
2003
Johns Hopkins University
2003
Malignant cells, like all actively growing must maintain their telomeres, but genetic mechanisms responsible for telomere maintenance in tumors have only recently been discovered. In particular, mutations of the binding proteins alpha thalassemia/mental retardation syndrome X-linked ( ATRX ) or death-domain associated protein DAXX shown to underlie a mechanism not involving telomerase (alternative lengthening telomeres), and point promoter reverse transcriptase TERT gene increase expression...
The proteins encoded by ATRX and DAXX participate in chromatin remodeling at telomeres other genomic sites. Because inactivating mutations of these genes are common human pancreatic neuroendocrine tumors (PanNETs), we examined the telomere status tumors. We found that 61% PanNETs displayed abnormal characteristic a telomerase-independent maintenance mechanism termed ALT (alternative lengthening telomeres). All exhibiting had or loss nuclear protein. also correlate with central nervous...
Many areas of biomedical research depend on the analysis uncommon variations in individual genes or transcripts. Here we describe a method that can quantify such variation at scale and ease heretofore unattainable. Each DNA molecule collection molecules is converted into single magnetic particle to which thousands copies identical sequence original are bound. This population beads then corresponds one-to-one representation starting molecules. Variation within be simply assessed by counting...
Genomic analysis of a childhood cancer reveals markedly fewer mutations than what is typically seen in adult cancers.
Replicative immortality is a hallmark of cancer cells governed by telomere maintenance. Approximately 90% human cancers maintain their telomeres activating telomerase, driven the transcriptional upregulation telomerase reverse transcriptase (TERT). Although TERT promoter mutations (TPMs) are major cancer-associated genetic mechanism upregulation, many exhibit without TPMs. In this study, we describe hypermethylated oncological region (THOR), 433-bp genomic encompassing 52 CpG sites located...
The majority of glioblastomas can be classified into molecular subgroups based on mutations in the TERT promoter (TERTp) and isocitrate dehydrogenase 1 or 2 (IDH). These utilize distinct genetic mechanisms telomere maintenance, either TERTp mutation leading to telomerase activation ATRX-mutation an alternative lengthening telomeres phenotype (ALT). However, about 20% lack alterations IDH. tumors, designated TERTpWT-IDHWT glioblastomas, do not have well-established biomarkers defined...
Green synthesis of metal nanoparticles (NPs) has received extensive attention over other conventional approaches due to their non-toxic nature and more biocompatibility. Herein we report gold silver NPs (AuNPs@AV AgNPs@AV) prepared by employing a green approach using crude extract Aconitum violaceum Jacquem. ex Stapf. The synthesized were characterized Scanning Electron Microscopy (SEM), Transmission (TEM), Energy Dispersive X-ray (EDX), Diffraction (XRD), UV/Visible spectroscopy, Fourier...
Abstract OTX2 is a developmentally regulated transcription factor involved in early morphogenesis of the central nervous system. This gene amplified and overexpressed medulloblastoma cell lines, but nature extent its genetic alterations primary tumors have not been evaluated. Analysis large cohort medulloblastomas revealed frequent focal copy number gain region minimally containing as single gene. was restricted to tumor subtypes that did express molecular signature Wnt or Shh pathway...
Although medulloblastoma is the most common pediatric malignant brain tumor, its molecular underpinnings are largely unknown. We have identified rare, recurrent homozygous deletions of Kruppel-like Factor 4 (KLF4) in using high-resolution single nucleotide polymorphism arrays, digital karyotyping, and genomic real-time polymerase chain reaction (PCR). Furthermore, we show that there loss physiological KLF4 expression more than 40% primary medulloblastomas both at RNA protein levels....
Sphingopyxis sp. USTB-05, which we previously identified and examined, is a well-known bacterial strain for biodegrading cyanobacterial hepatotoxins of both nodularins (NODs) microcystins (MCs). Although the pathways different types [D-Asp1] NOD, MC-YR, MC-LR MC-RR by USTB-05 were suggested, several biodegradation genes successfully cloned expressed, comprehensive genomic analysis was not reported. Here, based on second third generation sequencing technology, analyzed whole genome 4,679,489...
Aberrant activation of telomerase in human cancer is achieved by various alterations within the TERT promoter, including cancer-specific DNA hypermethylation hypermethylated oncological region (THOR). However, impact allele-specific methylation promoter on gene transcription remains incompletely understood. Using next-generation sequencing, we screened a large cohort normal and tumor tissues (n = 652) from 10 types identified that differential allelic (DAM) THOR restricted to cancerous...
BACKGROUND: Gliomas are a deadly group of childhood and adult cancers associated with high relapse rate following current therapies. Limitless self-renewal is hallmark cancer recurrence controlled by telomerase activation telomere maintenance. We have recently uncovered THOR (TERT Hypermethylated Oncological Region) which paradoxically hypermethylated in gliomas activation. In order to further explore the biological impact hypermethylation on self renewal maintenance we undertook stepwise...
Abstract Malignant tumor cells overcome the restriction of progressive telomere shortening by employing maintenance mechanisms (TMMs) to achieve replicative immortality. Over 80% human cancers maintain length using enzyme telomerase, which uses an RNA template subunit. However, other their telomeres in a telomerase-independent manner, also known as Alternative Lengthening Telomeres (ALT). Recently, we identified loss-of-function mutations SMARCAL1 gene novel genetic alterations associated...
ABSTRACT Sphingomonadaceae has a large number of strains that can biodegrade hepatotoxins or environmental pollutants. The latest research reported certain also produce lutein. Based on the third-generation sequencing technology, we analyzed whole genome sequence and compared related functional genes two isolated from different habitats. Sphingopyxis USTB-05 was 4,679,489 bp contained 4312 protein coding genes. 4,239,716 nuclear Sphingomonas morindae NBD5, harboring 3882 genes, sets...