A5054808938- Autophagy in Disease and Therapy
- HIV Research and Treatment
- Heat shock proteins research
- CRISPR and Genetic Engineering
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- HIV/AIDS drug development and treatment
- Pluripotent Stem Cells Research
- Alkaloids: synthesis and pharmacology
- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Traditional and Medicinal Uses of Annonaceae
- Genetics, Aging, and Longevity in Model Organisms
- Alzheimer's disease research and treatments
- Protein Hydrolysis and Bioactive Peptides
- Photosynthetic Processes and Mechanisms
- Plant Genetic and Mutation Studies
- interferon and immune responses
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Nerve injury and regeneration
- Berberine and alkaloids research
- Cardiovascular Effects of Exercise
- Natural product bioactivities and synthesis
Chinese Academy of Sciences
2015-2025
Soochow University
2022-2025
Hefei Institutes of Physical Science
2025
Institute of Solid State Physics
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2025
Kunming Institute of Botany
2019-2024
Shanghai Institute of Materia Medica
2024
University of Chinese Academy of Sciences
2015-2024
West China Medical Center of Sichuan University
2024
Howard Hughes Medical Institute
2023-2024
Abstract Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop breeding. The avoidance of transgene integration and reduction off-target mutations most important targets optimization. Here, we describe an efficient genome editing method bread wheat using ribonucleoproteins (RNPs). Starting from RNP preparation, whole protocol takes only seven nine weeks, with four five independent mutants produced 100 immature embryos. Deep sequencing reveals that chance in...
ABSTRACT A recently approved peptidic human immunodeficiency virus type 1 (HIV-1) fusion inhibitor, T-20 (Fuzeon; Trimeris Inc.), has shown significant promise in clinical application for treating HIV-1-infected individuals who have failed to respond the currently available antiretroviral drugs. However, must be injected twice daily and is too expensive. Therefore, it essential develop orally small molecule HIV-1 inhibitors. By screening a chemical library consisting of “drug-like”...
Autophagy plays important roles in modulating viral replication and antiviral immune response. Coronavirus infection is associated with the autophagic process, however, little known about mechanisms of autophagy induction its contribution to coronavirus regulation host innate responses. Here, we show that membrane-associated papain-like protease PLP2 (PLP2-TM) coronaviruses acts as a novel autophagy-inducing protein. Intriguingly, PLP2-TM induces incomplete process by increasing accumulation...
Abstract Lysosomes are key cellular organelles that metabolize extra- and intracellular substrates. Alterations in lysosomal metabolism implicated ageing-associated metabolic neurodegenerative diseases. However, how actively coordinates the nervous systems to regulate ageing remains unclear. Here we report a fat-to-neuron lipid signalling pathway induced by its longevity-promoting role Caenorhabditis elegans . We discovered lipolysis peripheral fat storage tissue upregulates neuropeptide...
Lysosomes are active sites to integrate cellular metabolism and signal transduction. A collection of proteins associated with the lysosome mediate these metabolic signaling functions. Both lysosomal have been linked longevity regulation; however, how lysosomes adjust their protein composition accommodate this regulation remains unclear. Using deep proteomic profiling, we systemically profiled lysosome-associated four different mechanisms. We discovered recruitment AMP-activated kinase...
Pluripotent stem cells, including induced pluripotent and embryonic cells (ESCs), have less developed mitochondria than somatic and, therefore, rely more heavily on glycolysis for energy production.Citation1-3 However, how mitochondrial homeostasis matches the demands of nuclear reprogramming regulates pluripotency in ESCs is largely unknown. Here, we identified ATG3-dependent autophagy as an executor both remodeling during cell regulation ESCs. Dysfunctional by Atg3 deletion inhibited...
Obstructive sleep apnea (OSA) can result in hypertension and significantly increase cardiovascular morbidity mortality. There are few reports on the long-term effects of continuous positive airway pressure (CPAP) blood patients with uncontrolled coronary heart disease (CHD) OSA. We conducted a prospective, follow-up study 83 hypertension, CHD, OSA randomized to control or CPAP groups. Daytime systolic (SBP), diastolic (DBP), severe cerebrovascular events (SCCEs) were recorded at baseline...
Abstract Autophagy-mediated mitochondrial degradation plays pivotal roles in both the acquisition and maintenance of pluripotency, but molecular mechanisms that link autophagy-mediated homeostasis to pluripotency regulation are unclear. Here, we identified mitophagy receptor BNIP3 regulates pluripotency. In mouse ESCs, depletion caused accumulation aberrant mitochondria accompanied by decreased membrane potential, increased production reactive oxygen species (ROS), reduced ATP generation,...
Hypertrophic lysosomes are critical for tumor progression and drug resistance; however, effective specific lysosome-targeting compounds cancer therapy lacking. Here we conducted a lysosomotropic pharmacophore-based in silico screen natural product library (2,212 compounds), identified polyphyllin D (PD) as novel lysosome-targeted compound. PD treatment was found to cause lysosomal damage, evidenced by the blockade of autophagic flux, loss lysophagy, release contents, thus exhibiting...
Surface-enhanced Raman scattering (SERS) is an ultrasensitive optical technique that critical for protein detection and essential identifying structure concentrations in various biomedical diagnostic applications. However, achieving highly sensitive reproducible SERS signals label-free proteins remains challenging due to their weak structural complexity. In this study, silver nanomushroom arrays (Ag NMAs) as substrates were readily prepared surface-engineered using a facile template-assisted...
MicroRNAs (miRNAs) are short, single-stranded and non-coding RNAs, freely circulating in human plasma correlating with vary pathologies. In this study, we monitored early myocardial injury recovery after heart transplantation by detecting levels of muscle-specific miR-133a, miR-133b miR-208a.7 consecutive patients underwent Fuwai hospital 14 healthy controls were included our study. Peripheral vein blood was drawn from on the day just (day 0), 1st, 2nd, 3rd, 7th 14th respectively. Serum...