A5079141970- Cerebrospinal fluid and hydrocephalus
- Neonatal and fetal brain pathology
- Traumatic Brain Injury and Neurovascular Disturbances
- Fetal and Pediatric Neurological Disorders
- Neonatal Respiratory Health Research
- Traumatic Brain Injury Research
- Advanced Neuroimaging Techniques and Applications
- S100 Proteins and Annexins
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Arrest and Resuscitation
- Neuroinflammation and Neurodegeneration Mechanisms
- Human Rights and Immigration
- Bacterial Infections and Vaccines
- Employment, Labor, and Gender Studies
- Glioma Diagnosis and Treatment
- Spinal Dysraphism and Malformations
- Neurological Disorders and Treatments
- History and Politics in Latin America
- Neurosurgical Procedures and Complications
- Intracerebral and Subarachnoid Hemorrhage Research
- Intracranial Aneurysms: Treatment and Complications
- Cancer Treatment and Pharmacology
- Microbial Metabolites in Food Biotechnology
- Latin American socio-political dynamics
- Chemical and Environmental Engineering Research
Washington University in St. Louis
2015-2024
Neurological Surgery
2011-2022
St. Louis Children's Hospital
2017-2021
University of Calgary
2021
Mallinckrodt (United States)
2021
Children's Hospital of Orange County
2021
National University of Engineering
2020
Instituto de Investigación en Ciencias de la Alimentación
2019
Universidad Autónoma de Madrid
2019
Neurology, Inc
2019
To determine if ventricular zone (VZ) and subventricular (SVZ) alterations are associated with intraventricular hemorrhage (IVH) posthemorrhagic hydrocephalus, we compared postmortem frontal subcortical brain samples from 12 infants IVH 3 nonneurological disease controls without hemorrhages or ventriculomegaly. Birth expiration estimated gestational ages were 23.0–39.1 23.7–44.1 weeks, respectively; survival ranges 0–42 days (median, 2.0 days). Routine histology immunohistochemistry for...
Abstract We sought to evaluate the potential of C17.2 neural progenitor cells (NPCs) engineered secrete glial cell line‐derived neurotrophic factor (GDNF) survive, differentiate and promote functional recovery following engraftment into brains adult male Sprague–Dawley rats subjected lateral fluid percussion brain injury. First, we demonstrated continued cortical expression GDNF receptor components (GFRα‐1, c‐Ret), suggesting that could have a physiological effect in immediate post‐traumatic...
Neuroinflammation has been implicated in the pathophysiology of post-hemorrhagic hydrocephalus (PHH) prematurity, but no comprehensive analysis signaling molecules performed using human cerebrospinal fluid (CSF). Lumbar CSF levels key cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12, TNF-α, TGF-β1, IFN-γ) and chemokines (XCL-1, CCL-2, CCL-3, CCL-19, CXCL-10, CXCL-11, CXCL-12) were measured conventional multiplexed Enzyme-linked Immunosorbent Assays compared between preterm infants...
Object This study describes the pediatric experience with a dual-multifunction-room IMRIS 1.5-T intraoperative magnetic resonance imaging (iMRI) suite and analyzes its impact on clinical variables associated neurosurgical resection of intracranial lesions, including safety efficacy. Methods Since inception iMRI–guided program in April 2008 at both Barnes-Jewish St. Louis Children's Hospital, prospective database recorded demographics outcome institutional review board approval. A similarly...
Abstract Intraventricular hemorrhage (IVH) is the most common cause of pediatric hydrocephalus in North America but remains poorly understood. Cell junction-mediated ventricular zone (VZ) disruption and astrogliosis are associated with pathogenesis congenital, nonhemorrhagic hydrocephalus. Recently, our group demonstrated that VZ also present preterm infants IVH. On basis this observation, we hypothesized blood triggers loss cell junction integrity related cytopathology. In order to test...
We conducted PrediXcan analysis of hydrocephalus risk in ten neurological tissues and whole blood. Decreased expression MAEL the brain was significantly associated (Bonferroni-adjusted p < 0.05) with hydrocephalus. imaging genomics data independent UK Biobank (N = 8,428) revealed that frontal cortex is white matter total volumes. Among top differentially expressed genes brain, we observed a significant enrichment for gene-level associations these structural phenotypes, suggesting an effect...
Hydrocephalus is a neurological disease with an incidence of 80-125 per 100,000 births in the United States. Neuropathology comprises ventriculomegaly, periventricular white matter (PVWM) alterations, inflammation, and gliosis. We hypothesized that hydrocephalus pig model associated subventricular PVWM cellular alterations neuroinflammation could mimic neuropathology described hydrocephalic infants.Hydrocephalus was induced by intracisternal kaolin injections 35-day old female pigs (n = 7...
Background and Purpose— Preterm neonates with intraventricular hemorrhage (IVH) are at risk for posthemorrhagic hydrocephalus poor neurological outcomes. Iron has been implicated in ventriculomegaly, hippocampal injury, outcomes following IVH. We hypothesized that levels of cerebrospinal fluid blood breakdown products endogenous iron clearance proteins IVH differ from those who subsequently develop hydrocephalus. Methods— Premature an estimated gestational age birth <30 weeks underwent...
Neurological outcomes of preterm infants with posthemorrhagic hydrocephalus are among the worst in newborn medicine. There remains no consensus regarding diagnosis or treatment hydrocephalus, and pathological pathways leading to adverse neurological sequelae poorly understood. In current study, we developed an innovative approach simultaneously identify potential diagnostic markers investigate novel hydrocephalus-related disability. Tandem multi-affinity fractionation for specific removal...
Many animal models have been used to study the pathophysiology of hydrocephalus; most these rodent whose lissencephalic cerebral cortex may not respond ventriculomegaly in same way as gyrencephalic species and size is amenable evaluation clinically relevant neurosurgical treatments. Fewer hydrocephalus used; thus, we expanded upon a porcine model juvenile pigs it explore surgical treatment methods.Acquired was induced 33-41-day old by percutaneous intracisternal injections kaolin (n = 17)....
Objective Iron has been implicated in the pathogenesis of brain injury and hydrocephalus after preterm germinal matrix hemorrhage‐intraventricular hemorrhage, however, it is unknown how external or endogenous intraventricular clearance iron pathway proteins affect outcome this group. Methods This prospective multicenter cohort included patients with posthemorrhagic (PHH) who underwent (1) temporary permanent cerebrospinal fluid (CSF) diversion (2) Bayley Scales Infant Development‐III testing...
Axonal injury is a hallmark of traumatic brain (TBI) and associated with poor clinical outcome. Following central nervous system injury, axons regenerate poorly, in part due to the presence molecules myelin that inhibit axonal outgrowth, including myelin-associated glycoprotein (MAG). The involvement MAG neurobehavioral deficits tissue loss following experimental TBI remains unexplored was evaluated current study using an MAG-specific monoclonal antibody (mAb). Anesthetized rats (n=102) were...
Density and viscosity measurements in the T = (273.15 to 318.15) K range of pure chloroform methyl tert-butyl ether (MTBE), as well binary system [x1 + (1 − x1) MTBE] over whole concentration at 293.15 K, were made. The experimental results for components fitted empirical equations, which permit calculation these properties studied temperature range. Calculated values are agreement with ones. Data mixture further used calculate excess molar volume deviations. enthalpy (303 ± 1) vapor−liquid...
Background Neurological outcomes of preterm infants with post-hemorrhagic hydrocephalus (PHH) remain among the worst in infancy, yet there few instruments to inform treatment PHH. We previously observed PHH-associated elevations cerebrospinal fluid (CSF) amyloid precursor protein (APP), neural cell adhesion molecule-L1 (L1CAM), molecule-1 (NCAM-1), and other mediators neurodevelopment. Objective The objective this study was examine association CSF APP, L1CAM, NCAM-1 ventricular size as an...
Intraventricular hemorrhage (IVH) is the most frequent, severe neurological complication of prematurity and associated with posthemorrhagic hydrocephalus (PHH) in up to half cases. PHH requires lifelong neurosurgical care significant cognitive psychomotor disability. Cerebrospinal fluid (CSF) biomarkers may provide both diagnostic information for novel insights into its pathophysiology.To explore ability candidate CSF PHH.Concentrations amyloid precursor protein (APP), soluble APPα (sAPPα),...
Abstract Background Severe intraventricular hemorrhage (IVH) is one of the most devastating neurological complications in preterm infants, with majority suffering long-term morbidity and up to 50% developing post-hemorrhagic hydrocephalus (PHH). Despite importance this disease, its cytopathological mechanisms are not well known. An vitro model IVH required investigate effects blood components on ventricular zone (VZ) stem cell niche. To address need, we developed a protocol from our accepted...
Abstract Background Approximately 30% of cerebrospinal fluid (CSF) shunt systems for hydrocephalus fail within the first year and 98% all patients will have failure in their lifetime. Obstruction remains most common reason failure. Previous evidence suggests elevated pro-inflammatory cytokines CSF are associated with worsening clinical outcomes neuroinflammatory diseases. The aim this study was to determine whether matrix metalloproteinases (MMPs) contribute towards hydrocephalus. Methods...
Interpretation of cerebrospinal fluid (CSF) studies can be challenging in preterm infants. We hypothesized that intraventricular hemorrhage (IVH), post-hemorrhagic hydrocephalus (PHH), and infection (meningitis) promote pro-inflammatory CSF conditions reflected parameters. Biochemical cytological profiles lumbar peripheral blood samples were analyzed for 81 control, 29 IVH grade 1/2 (IVH1/2), 13 3/4 (IVH3/4), 15 PHH, 20 culture-confirmed bacterial meningitis (BM), 27 viral (VM) infants at...
As deficits in memory and cognition are commonly observed survivors of traumatic brain injury (TBI), causing reduced quality life for the patient, a major goal experimental TBI research is to identify evaluate cognitive dysfunction. The present study assessed applicability serial Morris water maze (MWM) test determine function following same group rats which particularly important long-term studies increasingly valuable evaluation novel treatment strategies.Male Sprague-Dawley (n = 27) were...
Objectives: Posttraumatic hypotension is believed to increase morbidity and mortality in traumatically brain-injured patients. Using a clinically relevant model of combined traumatic brain injury with superimposed hemorrhagic rats, the present study evaluated whether reduction mean arterial blood pressure aggravates regional edema formation, cell death, neurologic motor/cognitive deficits associated injury. Design: Experimental prospective, randomized rodents. Setting: laboratory at...
Hydrocephalus is a complex neurological disorder with pervasive impact on the central nervous system. Previous work has demonstrated derangements in biochemical profile of cerebrospinal fluid (CSF) hydrocephalus, particularly infants and children, whom neurodevelopment progressing parallel concomitant injury. The objective this study was to examine CSF children congenital hydrocephalus (CHC) gain insight into pathophysiology identify candidate biomarkers CHC potential diagnostic therapeutic...