In Brief Study Design. vitro studies of the effects proinflammatory cytokines on production nerve growth factor (NGF) by human intervertebral disc (IVD) cells. Objective. To determine constitutive expression and NGF effect IVD Summary Background Data. may play a role in collateral sprouting sensory axons, neural survival, regulation nociceptive neurons. is known to be up-regulated cytokines. Methods. The presence protein was analyzed immunohistochemistry using cells obtained from cadaveric...

Abstract Objective Interleukin‐1 receptor antagonist (IL‐1Ra) has been evaluated for the intraarticular treatment of osteoarthritis. Such administration proteins may have limited utility because their rapid clearance and short half‐life in joint. The fusion a drug to elastin‐like polypeptides (ELPs) promotes formation aggregating particles that form “drug depot” at physiologic temperatures, phenomenon intended prolong presence drug. purpose this study was develop an injectable depot composed...

In Brief Study Design. vitro assessment of the effects platelet-rich plasma on extracellular matrix metabolism porcine intervertebral disc cells. Objectives. To determine whether is effective in stimulating cell proliferation and by cells cultured alginate beads. Summary Background Data. Platelet-rich used to accelerate wound healing tissue regeneration. Activated platelets release multiple growth factors that regulate proliferation, differentiation, morphogenesis. Individual present have...

Abstract Erythrocytes of diabetic subjects (non‐insulin dependent) were found to have eight‐ ten‐fold higher levels endogenously formed thiobarbituric acid reactive malonyldialdehyde (MDA), thirteen‐fold phospholipid‐MDA adduct, 15–20% reduced Na + ‐K ‐ATPase activity with unchanged Ca +2 activity, as compared the erythrocytes from normal healthy individuals. Incubation elevated concentrations (15–35 mM) glucose, similar that present in plasma, led increased lipid peroxidation, adduct...

Abstract Introduction Intervertebral disc (IVD) degeneration is associated with proteolytic degradation of the extracellular matrix, and its repair requires both production matrix downregulation proteinase activity. These properties are several growth factors. However, use factors in clinical practice limited by their high cost. This cost can be circumvented using synthetic peptides, such as Link N, which stimulate synthesis proteoglycan collagen IVD cells vitro . The purpose present study...

Purpose: Joint contractures in children with cerebral palsy contain muscle tissue that is mechanically stiffer higher collagen content than typically developing children. Interestingly, the correlation between and stiffness weak. To date, no data are available on types or other extracellular matrix proteins these muscles, nor any information regarding their function. Thus, our purpose was to measure specific protein composition human muscles along structural aspects of architecture determine...

Cerebral palsy (CP) is the most common cause of pediatric neurodevelopmental and physical disability in United States. It defined as a group motor disorders caused by nonprogressive perinatal insult to brain. Although brain lesion nonprogressive, there progressive, lifelong impact on skeletal muscles, which are shorter, spastic, may develop debilitating contractures. Satellite cells resident muscle stem that indispensable for postnatal growth regeneration muscles. Here we measured myogenic...

Rotator cuff pathology is a common source of shoulder pain with variable etiology and pathoanatomical characteristics. Pathological processes fatty infiltration, muscle atrophy, fibrosis have all been invoked as causes for poor outcomes after rotator tear repair. The aims this study were to measure the expression key genes associated adipogenesis, myogenesis, in human injury compare among groups patients varied severities pathology.Biopsies supraspinatus obtained arthroscopically from...

Abstract Aim To evaluate whether serum metabolomics differ between ambulatory individuals with cerebral palsy (CP) compared typical development and functional capacity is associated metabolite abundance. Method Thirty‐eight adolescents young adults were enrolled (CP: n = 19; development: 19). After testing (10‐meter walk, sit‐to‐stand, peak knee flexion/extension torques), blood was drawn. Targeted on hydrophilic metabolites performed by high‐performance liquid chromatography coupled...

This in vitro study used the alginate bead culture system to probe for differences effects of fibronectin fragment on cell proliferation and proteoglycan metabolism by different populations intervertebral disc cells articular chondrocytes.To compare proliferation, synthesis degradation from nucleus pulposus, anulus fibrosus, cartilage.In cartilage, administration stimulates cartilage degeneration. Fibronectin levels were increased human discs with injected into central region rabbit induced...

Fundamental questions about patient heterogeneity and human-specific pathophysiology currently obstruct progress towards a therapy for traumatic brain injury (TBI). Human in vitro models have the potential to address these questions. Three-dimensional spheroidal cell culture protocols human-origin neural cells several important advantages over their two-dimensional monolayer counterparts. cultures may mature more quickly, develop biofidelic electrophysiological activity and/or reproduce some...

Abstract Background Progressive functional decline is a key element of cancer-associated cachexia. No therapies have successfully translated to the clinic due an inability measure and improve physical function in cachectic patients. Major barriers translating pre-clinical include lack cancer models that accurately mimic use non-specific outcome measures function, like grip strength. New approaches are needed investigate cachexia-related at both basic clinical science levels. Methods Survival...

Detrimental changes in the composition and function of rotator cuff (RC) muscles are hallmarks RC disease progression. Previous studies have demonstrated both atrophic degenerative muscle loss advanced disease. However, relationship between gene expression pathology remains poorly defined, large part due to a lack correlating tissue composition. Therefore, purpose this study was determine how relates biopsies from patients undergoing reverse shoulder arthroplasty (RSA). Gene related...

Osteoarthritis (OA) is the most common and debilitating joint disease of advanced age has no universally effective therapy. Here, we demonstrate that systemic transplantation adult multipotent muscle-derived stem/progenitor cells (MDSPCs)-isolated from young mice-rejuvenates knee articular cartilage (AC) naturally aged mice. This intervention reduced expression pro-inflammatory cytokines (Tnf Il1a) catabolic matrix-degrading proteinases (Mmp3 Mmp13) in cartilage. Treatment with MDSPCs also...

ABSTRACT Background Biomarkers have potential to identify early signs of joint disease. This study compared pain and function in adolescents young adults with CP individuals without. Methods Cross-sectional CP(n = 20), aged 13-30 Gross Motor Function Classification System (GMFCS) I-III age-matched without 20). Knee hip measured using Numeric Pain Rating Scale (NPRS) injury Osteoarthritis Outcome Score (KOOS) Hip dysfunction (HOOS) surveys. Objective strength were also measured. for tissue...

In Brief Study Design. Immunohistochemical and biochemical analyses of NG2 proteoglycan in the human intervertebral disc. Objective. To determine if disc expresses proteoglycan. Summary Background Data. nervous system, has been reported to play an important role as interactive extracellular matrix component membrane receptor for growth factors. is also found non-neuronal tissues, such cartilage bone; however, expression within unknown. Methods. was examined through Western blotting, reverse...

Abstract Background Osteoarthritis (OA) is one of the most prevalent joint diseases advanced age and a leading cause disability worldwide. Ageing major risk factor for articular cartilage (AC) degeneration that leads to OA, age-related decline in regenerative capacity accelerates OA progression. Here we demonstrate systemic transplantation unique population adult multipotent muscle-derived stem/progenitor cells (MDSPCs), isolated from young wild-type mice, into Zmpste24 −/− mice (a model...

OBJECTIVE: To evaluate the incidence, trends, risk factors and clinical outcome of ectopic pregnancy in a tertiary referral institute.METHODS: Fifty four percent who presented to our department between April 2009 March 2010(1year period) were analyzed accordingly after confirming diagnosis.RESULTS: Among total 54 cases, 50(92.59%)were tubal pregnancies 1(1.8%) was ovarian 3(5.5%) ruptured corpus luteal hematoma which mistaken for pregnancy.Two (3.71%) cases noted following sterilization.Six...

Modern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested fixed tissues offer an incredible reservoir of untapped information. However, obtaining RNA from such formaldehyde-fixed tissues, especially fibrotic aged is technically challenging often results in degradation, chemical modification yield reduction, prohibiting further Therefore, we...