A5100527326- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Prenatal Screening and Diagnostics
- Cancer, Lipids, and Metabolism
- Congenital Heart Disease Studies
- HIV/AIDS Research and Interventions
- Mitochondrial Function and Pathology
- Cardiovascular Function and Risk Factors
- Obesity, Physical Activity, Diet
- Genomics and Rare Diseases
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Fetal and Pediatric Neurological Disorders
- Cancer-related molecular mechanisms research
- ATP Synthase and ATPases Research
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Nutritional Studies and Diet
- Cholinesterase and Neurodegenerative Diseases
- Cardiac Valve Diseases and Treatments
- Hypertrophic osteoarthropathy and related conditions
- Heat shock proteins research
- Genomic variations and chromosomal abnormalities
- Ovarian function and disorders
Zhongnan Hospital of Wuhan University
2009-2025
Wuhan University
2009-2025
Eye & ENT Hospital of Fudan University
2018-2025
Beijing YouAn Hospital
2022-2024
Capital Medical University
2022-2024
Lanzhou University
2023-2024
Weatherford College
2023
First Hospital of Lanzhou University
2023
Wuhan Medical Center for Women and Children
2021-2022
Shanghai Municipal Center For Disease Control Prevention
2018
The <i>mdm2</i> gene product is an important regulator of p53 function and stability. mdm2 E3 ubiquitin ligase for the RING finger domain critical activity. Ubiquitin (Ub) conjugation a general targeting modification poly-ubiquitin chains specifically target proteins to proteasome degradation. In this report, we show that multistep cascade mdm2-mediated ubiquitination can be reduced three purified recombinant proteins: ubiquitin-conjugated E2, mdm2, p53. This simplification allows enzymatic...
The aim was to assess the diagnostic accuracy of four different nutrition screening and evaluation instruments in nasopharyngeal carcinoma patients, benchmarked against Global Leadership Initiative on Malnutrition (GLIM) criteria. An observational, single-institution study conducted between June 2022 May 2023. Nutrition status assessed using Nutritional Risk Screening 2002 (NRS-2002), Universal Tool (MUST), (MST), Patient-Generated Subjective Assessment (PG-SGA), GLIM criteria at two time...
Mdm2 negatively regulates p53 by inhibiting its transcriptional activity and promoting degradation functioning as an E3 ubiquitin ligase. The primary binding site on mdm2 is located in N-terminal domain. Through to at transactivation domain, directly blocks the activation function of p53. We discovered that truncated protein constructs without domain are least active full-length catalyzing ubiquitination. Furthermore, deletion central acidic significantly reduces ligase toward have also...
Context Persons living with HIV (PLWH) commonly experience mental health symptoms. However, little is known about the core symptoms and their relationships. Objective This study aimed to evaluate prevalence of various explore relationships in symptom networks among PLWH. Methods From April July 2022, we recruited 518 participants through convenience sampling Beijing, China, for this cross-sectional study. Forty symptoms, including six dimensions (somatization negative affect, cognitive...
The incidence and mortality of gynecological tumors are progressively increasing due to factors such as obesity, viral infection, unhealthy habits, well social economic pressures.Consequently, it has emerged a significant threat women's health.Numerous studies have revealed the remarkable metabolic activity tumor cells in glycolysis its ability influence malignant biological behavior through specific mechanisms.Therefore, is crucial for patients gynecologists comprehend role glycolytic...
A complete cDNA clone encoding bovine mitochondrial translational initiation factor 2 (IF-2mt) has been obtained. The regions of the corresponding to mature IF-2mt and several its functional domains have expressed in Escherichia coli as histidine-tagged proteins. precursor (~90 kDa) (~85 forms are toxic E. can only be at low levels. Shorter this (~80 ~72 also found during expression IF-2mt. various separated by high performance liquid chromatography. All these active promoting GTP-dependent...
Tumor suppressor p53 is typically maintained at low levels in normal cells. In response to cellular stresses, such as DNA damage, stabilized and can stimulate responses leading cell cycle arrest or apoptosis. Corresponding its central role preventing propagation of damaged cells, mutation deletion found nearly 50% all human tumors. Mdm2 (mouse-d-minute 2) ortholog (hmdm2 hdm2) catalyze the ubiquitination p53, targeting it for degradation via proteosome. Thus, activity mdm2 inversely...
Abstract Objective To examine the acceptability and feasibility of using smartphone technology to assess beverage intake evaluate whether use is greater among key sub-populations. Design An study recording video dietary record, ecological momentary assessment (EMA), wearing smartphones videos helped participants recall after a cross-over validation study. Setting Rural urban area in Shanghai, China. Subjects Healthy adults ( n 110) aged 20–40 years old. Results Most reported that phone was...
Abstract Objective To understand parents’ knowledge and use of nutrition labelling to explore its associated factors. Design Cross-sectional survey. Setting Two schools providing a nine-year educational programme in Putuo District, Shanghai, China, were selected for the study. Information was included on demographic data Chinese Food Pagoda. Subjects Students their parents ( n 1770) participated questionnaire Results Of questionnaires, 1766 completed (response rate 99·8 %). Utilization 19·3...
Abstract Background Congenital heart defect (CHD) is the most common congenital abnormality, and it has long been a clinical public health concern. Our previous findings have found Periostin (POSTN) Pappalysin-1 (PAPPA) as potential biomarkers for fetal CHD. We aim to further elucidate POSTN's role in development explore applicability of POSTN PAPPA diagnostic marker This study poised establish theoretical framework mitigating incidence CHD advance novel approach prenatal screening Methods...
Abstract Background Placental mosaicism is one of the major reasons for noninvasive prenatal testing (NIPT) discrepancy. Herein, we discovered a rare case placenta with complex karyotypes that caused false‐positive and false‐negative results in testing. Methods Next‐generation sequencing (NGS) Quantitative fluorescent polymerase chain reaction (QF‐PCR) were performed on cord blood sample, fetal tissues, eight placental biopsies. Fluorescent In Situ Hybridization (FISH) karyotyping also...
Background: A fetal bronchogenic cyst (BC) is a rare congenital anomaly with an incidence of 0.147–0.238‰. The coronavirus disease 2019 (COVID-19) pandemic, as particular situation, hindered pregnant women from receiving periodic prenatal checkups. Case Description: Until 34 +6 weeks gestation, case the intrathoracic was found by ultrasound examination. Further, MRI examination confirmed diagnosis mediastinal cystic lesion, probably BC. Genetic testing not conducted due to COVID-19 pandemic....
Bladder cancer (BLCA) is an extremely common carcinoma of the urinary system that has a high incidence relapse. Although intensive studies have investigated its pathology in past decades, there are significant knowledge gaps regarding characterization molecular processes underlying progression disease and consequently prognosis. The purpose current research was to identify genes could serve as prognostic biomarkers.Gene expression profiles were downloaded from Cancer Genome Atlas (TCGA) Gene...
Objective: The article aims to provide genetic counseling a family with two children who were experiencing growth and developmental delays. Methods: Clinical information of the proband was collected. Peripheral blood collected from core members identify initial reason for delays by whole exome sequencing (WES) Sanger sequencing. To ascertain consequences newly discovered variants, details variants detected analyzed bioinformatic tools. Furthermore, we performed in vitro experimentation...