3D printing technology offers extensive applications in tissue engineering and regenerative medicine (TERM) because it can create a three-dimensional porous structure with acceptable porosity fine mechanical qualities that mimic natural bone. Hydroxyapatite (HA) is commonly used as bone repair material due to its excellent biocompatibility osteoconductivity. Small extracellular vesicles (sEVs) derived from marrow mesenchymal stem cells (BMSCs) regulate metabolism stimulate the osteogenic...

Scaffold materials, seed cells and growth factors are referred to as the three elements of bone tissue engineering (BTE). However, physical properties biocompatibility these scaffolds safety cost their limit clinical application. Therefore, it is critical develop a novel scaffold modified with efficient that meets requirements BTE. Herein, 3D-printed poly (lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA) composite new fusion peptides, which can effectively promote regeneration repair...

Rheumatoid arthritis (RA) is an inflammatory joint disorder, the progression of which leads to destruction cartilage and bone. Chemokines are involved in RA pathogenesis. In this study, we investigated chemokine signaling pathway associated with CCL2 peripheral blood (PB) synovial tissues (ST) patients based on our previous work about activation production collagen-induced rat ST. Total RNA was isolated from PB leukocytes synovium knee both control populations. Real-time polymerase chain...

Injectable thermo-sensitive hydrogels composed of small intestinal submucosa (SIS) with exosomes derived from bone marrow mesenchymal stem cells (BMSCs) are desired for regeneration. However, poor mechanical properties limit the clinical application SIS hydrogels. Herein, incorporated 3-(3,4-dihydroxyphenyl) propionic acid (CA) assessed. The results show that improved. In addition, retention and stability over time at defect site also challenges. Fusion peptides designed by connecting...

The challenges in the treatment of extensive bone defects are infection control and regeneration. Bone tissue engineering is currently one most promising strategies. In this study, a short biopeptide with specific osteogenic ability designed by fusion peptide technology encapsulated chitosan-modified poly(lactic acid–glycolic acid) (PLGA) microspheres. (FP) mainly consists an functional sequence (P-15) bone-specific binding (Asp-6), which can regulate formation accurately efficiently....

Bone defects caused by severe trauma, tumors, infections and diseases remain a global challenge due to limited natural regeneration capacity of bone tissue in large-scale or complex injuries. Guided (GBR) has emerged as pivotal technique addressing these issues, relying on barrier membranes facilitate osteoprogenitor cell infiltration. Current clinical GBR function solely physical barriers, lacking antibacterial osteoinductive properties, which underscores the need for advanced alternatives....

Background: MicroRNAs (miRs) play a crucial role in inflammatory diseases, including periodontitis. Meanwhile, miRs act as biomarkers for predicting diabetes mellitus (DM). However, the regulatory mechanism of miR‐126 on development periodontitis patients with DM still remains unclear. Methods: Human gingival fibroblasts were cultured low (5.5 mmol/L), medium (15 and high (25 mmol/L) glucose, respectively. Expressions miR‐126, tumor necrosis factor (TNF) receptor associated (TRAF) 6, related...

Abstract Tissue injury, which often occurs in daily life, remains challenging clinical medicine. Developing a novel biomaterial with the capability to provide an ideal microenvironment and homeostasis around wound is highly desirable for effective tissue regenerative The small intestinal submucosa (SIS) membrane possesses precise spatial structure excellent biocompatibility. Extracellular vesicles (EVs) derived from umbilical cord mesenchymal stem cells can achieve rapid cell proliferation...

Abstract Guided bone regeneration (GBR) technology is the most widely used and stable method for defect repair. However, infectious limits application of this technique. Herein, a small intestinal submucosa (SIS) membrane modified by chimeric peptides as new type GBR developed efficacious tissue regeneration. Based on main components SIS are I III collagen, collagen binding TKKTLRT KELNLVY sequences to construct with healing‐promoting peptide Hst1 or antibacterial osteogenic JH8194, so...

Oral squamous cell carcinoma (OSCC), as a common type of oral malignancy, has an unclear pathogenesis. N

This study aimed to fabricate Hyaluronic Acid (HA)/parecoxib-loaded PLGA microspheres for the treatment of Temporomandibular Disorders (TMD) and investigate in vitro vivo effect microsphere system solve issues poor drug delivery short duration on concentration conventional TMD therapy.The were prepared by double emulsion (w/o/w) method. Various formulations compared terms particle size, loading rate encapsulation rate. Scanning Electron Microscopy (SEM), Differential Calorimetry (DSC) FT-IR...

Exosomes derived from bone marrow mesenchymal stem cells (BMSC-exos) have been shown triggering osteogenic differentiation and mineralization of MSCs, but exosomes administered via bolus injections are rapidly sequestered cleared. Therefore, we considered the implant as a new organ patient's body expected to find method treat with BMSC-exos in vivo directly. A fusion peptide (PEP), drug delivery system (DDS) which contained titanium-binding (TBP) possessing ability selectively bind titanium...

Restoring bone homeostasis is the key to treatment of osteoporosis. How increase osteogenic ability or inhibit osteoclast activity has always been a topic great concern. In recent years, short peptides with biological have received attention in repair. However, application still limited due lack stable and targeted delivery system. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles modified by alendronate (AL) transport (OGP) (AL-PLGA@P NPs) are designed. Benefiting from high affinity AL for...

Background: Periodontitis and benign prostatic hyperplasia (BPH) are all common chronic diseases with higher incidence in middle-aged old men. Several studies have indicated a potential association between periodontitis BPH, although the findings remain inconclusive. However, there is no mendelian randomization (MR) to assess this association. Methods: The 40 men who had received health check-ups were included an epidemiological study. Genetic data of BPH (13118 cases 72799 controls) (3046...

Periodontitis is an inflammatory and destructive disease caused by dental plaque, which can result in the immune microenvironment disorders loss of periodontal support tissue. In order to promote restoration local stability, a functional biomaterial Gelatin methacryloyl @MP196/exos based on characteristics occurrence designed.

Guided bone regeneration (GBR) is a promising strategy for repairing defects using bioactive membranes. In this study, new type of GBR membrane based on the small intestinal submucosa (SIS) was created, and its surface structure, cytological characteristics, defect repair ability were compared with commonly used Our results show that to Heal-all Dentium membranes, SIS has an asymmetric structure does not affect proliferation marrow mesenchymal stem cells (BMSCs). Instead, it increased their...

Scaffold materials, seed cells and growth factors are referred to as the three elements of bone tissue engineering (BTE). However, physical properties biocompatibility scaffolds, safety costs factors, which limit clinical applications. Therefore, it is critical develop a novel scaffold modified with efficient that meets requirements BTE. Herein, 3D-printed poly (lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA) composite scaffolds by new fusion peptides were developed, can effectively...

Calcium silicate-based bioceramics have been applied in endodontics as advantageous materials for years, many chemical components and new synthesizing methods were used to improve the base formulation of positively affecting sealers properties. Recently, a novel biomaterial formulation, grounded strontium silicate, has introduced market, offering potential advancements field.