A5000250306- Tactile and Sensory Interactions
- Amyloidosis: Diagnosis, Treatment, Outcomes
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Hormonal Regulation and Hypertension
- Robotics and Sensor-Based Localization
- Hand Gesture Recognition Systems
- Peptidase Inhibition and Analysis
- Muscle Physiology and Disorders
- Pancreatitis Pathology and Treatment
- Acupuncture Treatment Research Studies
- Laser Applications in Dentistry and Medicine
- Education and Learning Interventions
- vaccines and immunoinformatics approaches
- Exercise and Physiological Responses
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Advanced Vision and Imaging
- Renal and related cancers
- Robotic Locomotion and Control
- Pancreatic function and diabetes
- Trace Elements in Health
- Circadian rhythm and melatonin
- Industrial Vision Systems and Defect Detection
- Human Pose and Action Recognition
- Cell Adhesion Molecules Research
Toyama University Hospital
2021-2024
Kobe University
2014-2024
Kobe Tokiwa University
2011-2024
University of Toyama
2022-2023
Kumamoto University
1997-2023
Life Science Institute
2022-2023
Kobe University Hospital
2011-2021
Toyota Research Institute
2019-2020
Toyota Industries (United States)
2019
The University of Tokyo
2004-2017
Abstract Aldosterone-producing adenomas (APAs) are the commonest curable cause of hypertension. Most have gain-of-function somatic mutations ion channels or transporters. Herein we report discovery, replication and phenotype in neuronal cell adhesion gene CADM1 . Independent whole exome sequencing 40 81 APAs found intramembranous p.Val380Asp p.Gly379Asp variants two patients whose hypertension periodic primary aldosteronism were cured by adrenalectomy. Replication identified more with each...
Abstract Currently, there are no treatments for Alport syndrome, which is the second most commonly inherited kidney disease. Here we report development of an exon-skipping therapy using antisense-oligonucleotide (ASO) severe male X-linked syndrome (XLAS). We targeted truncating variants in exon 21 COL4A5 gene and conducted a type IV collagen α3/α4/α5 chain triple helix formation assay, vitro vivo treatment efficacy evaluation. show that skipping enabled trimer formation, leading to...
We present an approach to navigating a biped robot safely and efficiently through complicated environment of previously unknown obstacles terrain using only on-board sensing odometry. Sensing the is performed by pivoting laser scanner, which continues update representation as walks. Safe stepping motions are planned from this data follow user's command, given in form end goal, rough path, or joystick input. Results demonstrated on prototype several environments.
Complementary and alternative medicine (CAM) may provide patients with an to traditional medicine, but assessment of its efficacy is required. One CAM method, electroacupuncture (EA) treatment, a maneuver that utilizes stimulation acupuncture needles low-frequency microcurrent. To study the effect short-term EA, we evaluated differential expression genes induced by EA in mouse skeletal muscle for up 24 h. We then used RT-PCR confirm patterns six differentially expressed genes. Bioinformatics...
Studies on hereditary pancreatitis have provided evidence in favor of central role for trypsin activity the disease. Identification genetic variants trypsinogen linked protease to onset pancreatitis, and biochemical characterization proposed an enzymatic gain function as initiating mechanism. Mutations serine inhibitor Kazal type 1gene (SPINK1) are shown be associated with pancreatitis. We previously reported that Spink3 (a mouse homologue gene human SPINK1) deficient mice showed excessive...
Objectives:In Japan, sodium-glucose co-transporter type 2 (SGLT2) inhibitors have been reported to be associated with serious skin and subcutaneous tissue disorders.A post-marketing surveillance (PMS) study suggested that the association was specific for ipragliflozin and, a lesser extent dapagliflozin.These studies were performed confirm of 6 SGLT2 disorders in clinical setting, elucidate role melanin understand underlying mechanisms.Methods: The latest PMS records retrieved from Japanese...
This paper describes an online system for footstep planning using a 3D map reconstructed by visual odometry. consists of two key components: reconstruction via odometry from stereo image sequence to obtain dense local world model, and planner biped robots the map. Visual is method connect sequences 6DOF camera motion environment information. The described in this three depth calculation, flow calculation tracking raw features, estimation RANSAC. Using resulting data, optimal locations...
We describe a mobile manipulation hardware and software system capable of autonomously performing complex human-level tasks in real homes, after being taught the task with single demonstration from person virtual reality. This is enabled by highly robot, whole-body space hybrid position/force control, teaching parameterized primitives linked to robust learned dense visual embeddings representation scene, graph behaviors. demonstrate robustness approach presenting results for variety tasks,...
WT1 missense mutation in exon 8 or 9 causes infantile nephrotic syndrome with early progression to end-stage kidney disease (ESKD), Wilms tumor, and 46,XY female. However, some patients mutations progress ESKD their teens later. Therefore, we conducted a systematic review functional analysis of transcriptional activity.We 174 cases variants from our cohort (n=13) previous reports (n=161). Of these cases, mild severe genotypes were selected for further vitro using luciferase assay.The median...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses its S1 spike protein to bind angiotensin-converting enzyme (ACE2) on human cells in the first step of cell entry. Tryptanthrin, extracted from leaves indigo plant, Polygonum tinctorium, using d-limonene (17.3 µg/ml), is considered inhibit ACE2-mediated entry another type coronavirus, HCoV-NL63. The current study examined whether this extract could binding SARS-CoV-2 ACE2. Binding was quantified as cell-bound fluorescence...
Interleukin-13 (IL-13) is associated with allergic airway inflammation and remodeling. Our group found a variant single nucleotide polymorphism in the IL13 gene at position +2044G>A (rs20541) that was expected to result non-conservative replacement of positively charged arginine (R) neutral glutamine (Q) 144. IL-13Q144 augmented bronchial asthma There some indication anti-IL-13 monoclonal antibodies can demonstrate positive effect on clinical course refractory asthmatic patients. To date,...
Familial amyloidotic polyneuropathy is one type of protein misfolding disease. Transthyretin (TTR) tetramer dissociation the limiting step for amyloid fibril formation. CHF5074 (CSP‐1103) stabilizes TTR in vitro by binding to T4 site. Here, we used three strains double humanized mice (mTtr hTTRVal30/hTTRVal30 , mTtr hTTRVal30/hTTRMet30 and hTTRMet30/hTTRMet30 ) assess whether tetramers vivo. Treatment with increased serum levels stabilizing tetramers. Although affinities diflunisal TTRMet30...
Dystrophinopathy is caused by alterations in the dystrophin gene. The severe phenotype, Duchenne muscular dystrophy (DMD), a lack of skeletal muscles, resulting necrosis and regenerating fibers, inflammatory cells, muscle fibrosis. Progressive weakness characteristic finding this condition. Here, we encountered rare case 10-year-old patient with asymptomatic dystrophinopathy no expression investigated reason for absence to obtain therapeutic insights DMD. Using RNA-seq analysis, gene muscles...
Human erythrocyte protein 4.2 (band 4.2; pallidin) is a major membrane that comprises 5% of the total weight human membrane. Deficiencies this have been observed in hereditary spherocytosis with anaemia, suggesting role stability and integrity. The molecular basis disorder remains unknown. As first step elucidating pathogenesis associated deficiency, we cloned sequenced gene from normal Japanese person. We prepared sets oligonucleotide primers for polymerase chain reaction (PCR) determined...
Abstract Background Duchenne muscular dystrophy, a fatal muscle-wasting disease, is characterized by dystrophin deficiency caused mutations in the gene. Skipping of target exon during splicing with antisense oligonucleotides attracting much attention as most plausible way to express DMD. Antisense have been designed against regulatory sequences such enhancer exons. Recently, we reported that chemical kinase inhibitor specifically enhances skipping mutated 31, indicating existence...
Olmsted syndrome is a very rare congenital disorder, characterized by palmoplantar keratoderma and periorificial keratotic lesions. Recently, TRPV3 was reported to be causative gene of syndrome. We identified heterozygous missense mutation TRPV3, c.1703G>T, p.Gly568Val, in Japanese patient with To the best our knowledge, this first report harboring TRPV3. conducted silico analysis evaluate whether p.Gly568Val leads structural changes selectivity filter. The filter shown become dilated...