A5072110602- Plant Molecular Biology Research
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- DNA Repair Mechanisms
- GABA and Rice Research
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Plant Disease Resistance and Genetics
- Plant Stress Responses and Tolerance
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Microbial Metabolism and Applications
- Protist diversity and phylogeny
- Microbial Natural Products and Biosynthesis
- Plant Gene Expression Analysis
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
Yunnan Agricultural University
2012-2025
Nankai University
2017-2023
Lishui University
2020
Central China Normal University
2016
Chinese Academy of Sciences
2002-2004
Institute of Genetics and Developmental Biology
2002
Zen-Noh (Japan)
2000
Bin Han and colleagues report the draft genome sequence of grass carp Ctenopharyngodon idellus, a major commercially farmed species freshwater fish. Analyses identify lineage-specific duplications that may have contributed to adaptation this vegetarian diet. The is an important fish, accounting for ∼16% global aquaculture, has Here we 0.9-Gb gynogenetic female adult 1.07-Gb wild male adult. Genome annotation identified 27,263 protein-coding gene models in genome. A total 114 scaffolds...
In budding yeast, the ZMM complex is closely associated with class I crossovers and synaptonemal (SC) formation. However, relationship among genes remains unclear in most higher eukaryotes. Here, we identify rice ZIP4 homolog, a member of gene, explore its two other characterized genes, MER3 ZEP1. Our results show that zip4 mutant chiasma frequency greatly reduced, although synapsis proceeds only mild defects. Immunocytological analyses wild type reveal presents as punctuate foci...
Auxin is indispensable to the fertilization-induced coordinated development of embryo, endosperm, and seed coat. However, little attention has been given distribution pattern, maintenance mechanism, function auxin throughout process development. In present study, we found that response signals display a dynamic pattern during Arabidopsis Shortly after fertilization, strong were observed at funiculus, chalaza, micropylar integument where embryo attaches. Later, additional appeared middle...
Abstract Bub1 (for budding uninhibited by benzimidazole 1), one of the main spindle checkpoint kinases, acts as a kinetochore scaffold for assembling other proteins. Here, we identify plant Bub1-related kinase 1 (BRK1) in rice (Oryza sativa). The brk1 mutants are sterile due to precocious separation sister chromatids at onset anaphase I. centromeric recruitment SHUGOSHIN1 and phosphorylation histone H2A Thr-134 (H2A-pT134) depend on BRK1. Although homologs can faithfully separate from each...
The widespread introduction of semi-dwarf1 (sd1), also known as the 'Green Revolution' gene, has dramatically increased rice yield. However, extensive use limited sources dwarf genes may cause 'bottleneck' effects in breeding new varieties. Alternative germplasms are quite urgent for breeding. Here, we characterized a allele Slr1-d mutant, Slr1-d6, which reduced plant height by 37%, much milder dwarfism. Slr-d6 was still responsive to gibberellin (GA) extent. mutation site Slr1-d6 less...
Abstract MSH4 encodes a MutS protein that plays specialized role in meiosis. In eukaryotic species, such as budding yeast, mice, Caenorhabditis elegans, and Arabidopsis, msh4 mutants display meiotic defects with reduced number of chiasmata. Here, we characterized rice by map-based cloning. Osmsh4 mutants, the chiasma frequency was dramatically decreased to ∼10% wild type, but synaptonemal complex normally installed. The double mutant analysis showed Osmsh5 mutant, reduction chiasmata greater...
The BAG proteins are a family of multi-functional co-chaperones. In plants, were found to play roles both in abiotic and biotic stress tolerance. However, the function Arabidopsis BAG2 remains largely unknown, whereas BAG6 is required for plants’ defense pathogens, although it unknown whether involved tolerance stresses. Here, we show that expressed various tissues upregulated by salt, mannitol, heat treatments stress-related hormones including ABA, ethylene, SA. Germination bag2, bag6 bag2...
SDS is a meiosis specific cyclin-like protein and required for DMC1 mediated double-strand break (DSB) repairing in Arabidopsis. Here, we found its rice homolog, OsSDS, essential meiotic DSB formation. The Ossds mutant normal vegetative growth but both male female gametes are inviable. meiocytes exhibit severe defects homologous pairing synapsis. No γH2AX immunosignals together with the suppression of chromosome fragmentation Ossds-1 Osrad51c, provide strong evidences that OsSDS...
Abstract Background Serotonin, originally identified as a neurotransmitter in mammals, functions an antioxidant to scavenge cellular ROS plants. In rice, the conversion of tryptamine serotonin is catalyzed by SL (sekiguchi lesion), member cytochrome P450 monooxygenase family. The sl mutant, originated from rice cultivar Sekiguchi-asahi, exhibits spontaneous lesions, whereas its immune responses pathogens have not been clearly characterized. Results Here we three allelic mutants indica...
Abstract C2H2 zinc effectors are a class of pathogen proteins that play dual role in plant–pathogen interactions, promoting pathogenicity and enhancing plant defense. In our previous research, we identified Magnaporthe oryzae Systemic Defense Trigger 1 (MoSDT1) as effector activates rice (Oryza sativa) defense when overexpressed rice. However, its regulatory roles require further investigation. this study, generated an MoSDT1 overexpressing strain 2 knockout strains M. to assess the impact...
The wild relatives of rice have adapted to different ecological environments and constitute a useful reservoir agronomic traits for genetic improvement. Here we present the ~777 Mb de novo assembled genome sequence Oryza granulata. Recent bursts long-terminal repeat retrotransposons, especially RIRE2, led rapid twofold increase in size after O. granulata speciation. Universal centromeric tandem repeats are absent within its centromeres, while gypsy-type LTRs main centromere-specific...
Abstract Transposable elements exist widely throughout plant genomes and play important roles in evolution. Auxin is an regulator that traditionally associated with root development drought stress adaptation. The DEEPER ROOTING 1 ( DRO1 ) gene a key component of rice avoidance. Here, we identified transposon acts as autonomous auxin‐responsive promoter its presence at specific genome positions conveys physiological adaptations related to Rice varieties high auxin‐mediated transcription the...
Highly coordinated pollen wall patterning is essential for male reproductive development. Here, we report the identification of Defective Microspore Development 1 (DMD1), which encodes a nuclear-localized protein possessing transactivation activity. DMD1 preferentially expressed in tapetum and microspores during post-meiotic Mutations cause male-sterile phenotype with impaired microspore cell integrity. The mutants display abnormal callose degradation, accompanied by inhibited primexine...
Histone deacetylase (HDAC) inhibitors (HDACis) have been widely used in plants to investigate the role of histone acetylation, particularly function HDACs, regulation development and stress response. However, how acetylation is involved rice ( Oryza sativa L. ) disease resistance has hardly studied. In this paper, four HDACis including Sodium butyrate (NaBT), Suberoylanilide Hydroxamic Acid (SAHA), LBH-589 Trichostatin A (TSA) were treat seedlings at different concentrations before...
Homologous recombination is carefully orchestrated to maintain genome integrity. RAD51D has been previously shown be essential for double-strand break repair in mammalian somatic cells. However, the function of during meiosis largely unknown. Here, through detailed analyses Osrad51d single and double mutants, we pinpoint specific OsRAD51D coordinating homologous pairing by preventing nonhomologous interactions meiosis. associated with telomeres both meiocytes Loss leads significant induction...
Abstract Background Some of the pathogenic effector proteins play an active role in stimulating plant defense system to strengthen resistance. Results In this study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was implemented identify altered metabolites transgenic rice containing over-expressed M. oryzae Systemic Defense Trigger 1 (MoSDT1) that infected at three-time points. The characterized dominating were organic acids and...
Abstract The spindle assembly checkpoint (SAC) proteins are conserved among eukaryotes safeguarding chromosome segregation fidelity during mitosis. However, their biological functions in plant‐pathogenic fungi remain largely unknown. In this study, we found that the SAC protein MoMad1 rice blast fungus ( Magnaporthe oryzae ) localizes on nuclear envelope and is dispensable for M. vegetative growth tolerance to microtubule depolymerizing agent treatment. plays an important role...
Genes essential for gametophyte development and fertilization have been identified studied in detail; however, genes that fine-tune these processes are largely unknown. Here, we characterized an unknown Arabidopsis gene, GTP-BINDING PROTEIN RELATED1 (GPR1). GPR1 is specifically expressed ovule, pollen, pollen tube. Enhanced green fluorescent protein-tagged localizes to both nucleus cytoplasm, it also presents punctate ring-like structures. gpr1 mutants exhibit no defect gametogenesis seed...