A5057346968- Sleep and Wakefulness Research
- Neural dynamics and brain function
- Motor Control and Adaptation
- EEG and Brain-Computer Interfaces
- Neural and Behavioral Psychology Studies
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Sleep and related disorders
- Action Observation and Synchronization
- Geotourism and Geoheritage Conservation
- Muscle activation and electromyography studies
- Geological Modeling and Analysis
- Adipose Tissue and Metabolism
- X-ray Spectroscopy and Fluorescence Analysis
- Trace Elements in Health
- Hepatitis C virus research
- Pregnancy and preeclampsia studies
- Radioactivity and Radon Measurements
- Hepatitis B Virus Studies
- Neurobiology and Insect Physiology Research
- Birth, Development, and Health
- Neuroscience, Education and Cognitive Function
- Geographic Information Systems Studies
- Silk-based biomaterials and applications
- Synthesis and properties of polymers
University of Oxford
2019-2025
John Radcliffe Hospital
2021-2025
Jichi Medical University
2023-2024
Oxford Centre for Computational Neuroscience
2020
Tokyo Metropolitan Institute of Medical Science
2011-2017
Tamagawa University
2008-2017
Health Promotion Council
2014-2017
University of Tsukuba
2014
The University of Tokyo
2007-2014
Japan Science and Technology Agency
2012
Although the lateral prefrontal cortex (lPFC) and dorsal premotor (PMd) are thought to be involved in goal-directed behavior, specific roles of each area still remain elusive. To characterize compare neuronal activity two sectors lPFC [dorsal (dlPFC) ventral (vlPFC)] PMd, we designed a behavioral task for monkeys explore differences their participation four aspects information processing: encoding visual signals, goal retrieval, action specification, maintenance relevant information. We...
Before preparing to initiate a forthcoming motion, we often acquire information about the future action without specifying actual motor parameters. The for planning an at this conceptual level can be provided with verbal commands or nonverbal signals even before associated targets are visible. Under these conditions, signifying virtual plan must transformed that used constructing specific movements. To determine whether premotor cortex is involved in process, examined neuronal activity...
Growing evidence suggests that brain activity during sleep, as well sleep regulation, are tightly linked with synaptic function and network excitability at the local global levels. We previously reported a mutation in synaptobrevin 2 (Vamp2) restless (rlss) mice results marked increase of wakefulness suppression particular REM (REMS), increased consolidation wakefulness. In this study, using finer-scale vivo electrophysiology recordings, we report spontaneous cortical rlss NREM (NREMS) is...
Sleep and wakefulness are not simple, homogenous all-or-none states but represent a spectrum of substates, distinguished by behavior, levels arousal, brain activity at the local global levels. Until now, role hypothalamic circuitry in sleep-wake control was studied primarily with respect to its contribution rapid state transitions. In contrast, whether hypothalamus modulates within-state dynamics (state "quality") functional significance thereof remains unexplored. Here, we show that...
Multiple loop circuits interconnect the basal ganglia and frontal cortex, each part of cortico-basal loops plays an essential role in neuronal computational processes underlying motor behavior. To gain deeper insight into specific functions played by component loops, we compared response properties neurons globus pallidus (GP) with those dorsal premotor cortex (PMd) ventrolateral dorsolateral prefrontal (vlPFC dlPFC) while monkeys performed a behavioral task designed to include separate for...
Abstract Background Homeostatic regulation of sleep is reflected in the maintenance a daily balance between and wakefulness. Although numerous internal external factors can influence sleep, it unclear whether to what extent process that keeps track time spent awake determined by content waking experience. We hypothesised alterations environmental conditions may elicit different types wakefulness, which will turn both capacity sustain continuous wakefulness as well rates accumulating...
Electrophysiological recordings from freely behaving animals are a widespread and powerful mode of investigation in sleep research. These generate large amounts data that require stage annotation (polysomnography), which the is parcellated according to three vigilance states: awake, rapid eye movement (REM) sleep, non-REM (NREM) sleep. Manual current computational methods ignore intermediate states because classification features become ambiguous, even though contain important information...
Abstract Body temperature is an important physiological parameter in many studies of laboratory mice. Continuous assessment body has traditionally required surgical implantation a telemeter, but this invasive procedure adversely impacts animal welfare. Near-infrared thermography provides non-invasive alternative by continuously measuring the highest on outside (T skin ), reliability these recordings as proxy for continuous core ) measurements not been assessed. Here, T (30 s resolution) and...
Previous reports have indicated that the premotor cortex (PM) uses visual information for either direct guidance of limb movements or indirect specification action targets at a conceptual level. We explored how inputs signaling these two different categories are processed by PM neurons. Monkeys performed delayed reaching task after receiving sets instructions, one directly specifying spatial location motor target (a spatial-target cue) and other providing abstract about indicating whether to...
Animals need to discriminate differences in spatiotemporally distributed sensory signals terms of quality as well quantity for generating adaptive behavior. Olfactory characterized by odor identity and concentration are intermittently the environment. From these intervals stimulation, animals process odorant localize partners or food sources. Although concentration–response characteristics olfactory neurons have traditionally been investigated using single stimulus pulses, their behavior...
The dorsal premotor cortex residing in the dorsolateral aspect of area 6 is a rostrocaudally elongated that rostral to primary motor (M1) and caudal prefrontal cortex. This region, which subdivided into [pre-dorsal (pre-PMd)] [dorsal proper (PMd)] components, probably plays central role planning executing actions achieve behavioural goal. In present study, we investigated functional specializations pre-PMd, PMd, M1, because synthesis specific functions performed by each considered be...
Abstract Chronic hepatitis B virus (HBV) infection is a major medical concern worldwide. Current treatments for HBV effectively inhibit replication; however, these cannot cure and novel treatment-strategies should be necessary. In this study, we identified tripartite motif-containing protein 26 (TRIM26) could supportive factor replication. Small interfering RNA-mediated TRIM26 knockdown (KD) modestly attenuated replication in human hepatocytes. Endogenous physically interacted with core...
Abstract Background Approximately 296 million people have chronic hepatitis B (CHB) caused by virus (HBV). Current standard treatment, nucleos(t)ide analogs, are not efficient enough to eradicate HBV from the hepatocytes. Thus, developing new drugs for CHB is needed achieve complete cure. Methods Here we established a novel reporter system, HBV-HiBiT-PS2, screen CHB. HBV-HiBiT-PS2 was constructed adding an HiBiT-tag at 5′ end of preS2 and introduced this into HepG2-NTCP cells. Culture...
The microbrain of the silkmoth, Bombyx mori, is a model system for analyzing neural mechanisms underlying stimulus-driven behavior, and numerous studies using physiological morphological methods have accumulated. However, one limitations this lack methodology labeling specific subsets neurons. Targeted gene expression with GAL4/UAS system, which was recently developed, may overcome disadvantage. To test in silkmoth brain, we generated two GAL4 driver lines under control either bombyxin or...
The thalamic reticular nucleus (TRN) collects inputs from the cerebral cortex and thalamus and, in turn, sends inhibitory outputs to relay nuclei. This unique connectivity suggests that TRN plays a pivotal role regulating information flow through thalamus. Here, we analyzed roles of neurons visually guided reaching movements. We first used retrograde transneuronal labeling with rabies virus, showed rostro-dorsal sector (TRNrd) projected disynaptically ventral premotor (PMv). In other...
Abstract The cortex and subcortical circuitry are thought to play distinct roles in the generation of sleep oscillations global control vigilance states. Here we silenced a subset cortical layer 5 pyramidal dentate gyrus granule cells mice using cell-specific ablation key t-SNARE protein SNAP25. We found marked increase wakefulness accompanied by reduced rebound EEG slow-wave activity after deprivation. Our data illustrates an important role for both state homeostasis.
Abstract Sleep and wakefulness are not simple homogenous all-or-none states, but instead characterized by rich dynamics of brain activity across many temporal spatial scales. Rapid global state transitions between waking sleeping believed to be controlled hypothalamic circuits, the contribution hypothalamus within-state changes sleep wake “intensity” remains largely unexplored. Here we show that stimulation inhibitory neurons in preoptic does merely trigger awakening from sleep, resulting...
One of the most distinctive features mammalian cerebral cortex is its laminar structure. Of all cortical layers, layer 6b (L6b) by far least-studied, despite exhibiting direct sensitivity to orexin and having widespread connectivity, suggesting an important role in regulating oscillations brain state. We performed chronic electroencephalogram (EEG) recordings mice which a subset L6b neurons was conditionally silenced, during undisturbed conditions, after sleep deprivation (SD),...
ABSTRACT Growing evidence suggests that brain activity during sleep, as well sleep regulation, are tightly linked with synaptic function and network excitability at the local global levels. We previously reported a mutation in synaptobrevin 2 ( Vamp2 ) restless rlss mice results marked increase of wakefulness suppression particular REM (REMS) increased consolidation wakefulness. In current study, using finer-scale vivo electrophysiology recordings, we report spontaneous cortical NREM (NREMS)...
Abstract Continuous periods of wakefulness are associated with reduced performance levels due to the build-up sleep pressure in active regions brain. These effects manifest as use-dependent changes cortical network activity and mechanisms underlying these represent targets for overcoming cognitive tiredness. Here we reveal a central role intraneuronal chloride levels, which increase manner during waking, reduce strength local synaptic inhibition mouse cortex. Activity-dependent increases...