A5022460450- RNA modifications and cancer
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Epigenetics and DNA Methylation
- Macrophage Migration Inhibitory Factor
- Computational Drug Discovery Methods
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Advanced biosensing and bioanalysis techniques
- Viral-associated cancers and disorders
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Peptidase Inhibition and Analysis
- Toxin Mechanisms and Immunotoxins
- Antimicrobial Resistance in Staphylococcus
- Influenza Virus Research Studies
- Gut microbiota and health
Guangxi Medical University
2024
Samjin Pharm (South Korea)
2023
Columbia University
2016-2022
Tsinghua University
2009-2013
TNFα-targeting therapy with the use of drugs Etanercept, Infliximab, and Adalimumab is used in clinical treatment various inflammatory immune diseases. Although all these reagents function to disrupt interaction between TNFα its receptors, investigations showed advantages compared Etanercept Infliximab. However, underlying molecular mechanism action remains unclear. In our previous work, we presented structural data on how Infliximab binds E-F loop functions as a receptor-binding blocker. To...
The optimization, based on computational, thermodynamic, and crystallographic data, of a series small-molecule ligands the Phe43 cavity envelope glycoprotein gp120 human immunodeficiency virus (HIV) has been achieved. Importantly, biological evaluation revealed that CD4 mimics (4–7) inhibit HIV-1 entry into target cells with both significantly higher potency neutralization breadth than previous congeners, while maintaining high selectivity for virus. Their binding mode was characterized via...
Abstract As the most abundant messenger RNA (mRNA) modification, N6-methyladenosine (m6A) plays a crucial role in fate, impacting cellular and physiological processes various tumor types. However, our understanding of m6A methylome heterogeneity remains limited. Herein, we collected analyzed methylomes across nine human tissues from 97 sequencing (m6A-seq) (RNA-seq) samples. Our findings demonstrate that exhibits different compared to normal tissues, which contributes diverse clinical...
ABSTRACT Highly pathogenic influenza virus strains currently in circulation pose a significant risk of global pandemic. Following the reported crystal structure endonuclease domain from avian polymerase PA subunit, here we report results systematic X-ray crystallographic analysis its complex with adenosine, uridine, and thymidine nucleoside monophosphates (NMPs). Electron density corresponding to monophosphate moiety each nucleotide was apparent NMP bound catalytic metal. A hydrophobic site...
Gut Universe Database (GutUDB) provides a comprehensive, systematic, and practical platform for researchers, is dedicated to the management, analysis, visualization of knowledge related intestinal diseases. Based on this database, eight major categories omics data analyses are carried out explore genotype-phenotype characteristics certain disease. The first tool comprehensive research diseases will help each researcher better understand intestine serves as central organ in digestive system...
The design and synthesis of butyl chain derivatives at the indane ring 3-position our lead CD4-mimetic compound BNM-III-170 that inhibits human immunodeficiency virus (HIV-1) infection are reported. Optimization efforts were guided by crystallographic computational analysis small-molecule ligands Phe43 cavity envelope glycoprotein gp120. Biological evaluation 11-21 revealed members this series compounds able to inhibit HIV-1 viral entry into target cells more potently with greater breadth...
Staphylococcal enterotoxin (SE) B is among the potent toxins produced by Staphylococcus aureus that cause toxic shock syndrome (TSS), which can result in multi-organ failure and death. Currently, neutralizing antibodies have been shown to be effective immunotherapeutic agents against this toxin, but structural basis of mechanism still unknown. In study, we generated a monoclonal antibody, 3E2, SEB, analyzed crystal structure SEB-3E2 Fab complex. Crystallographic analysis suggested epitope...
The human immunodeficiency virus (HIV-1) envelope glycoprotein (Env) trimer on the virion surface interacts with host receptors, CD4 and CCR5/CXCR4, to mediate entry into target cell. CD4-mimetic compounds (CD4mcs) bind gp120 Env, block binding, inactivate Env. Previous studies suggested that a C(5)-methylamino methyl moiety lead CD4mc, BNM-III-170, contributed its antiviral potency. By replacing C(5) chain differentially substituted pyrrolidine, piperidine, piperazine ring systems, guided...
It is widely accepted that N6-methyladenosine (m6A) exhibits significant intercellular specificity, which poses challenges for its detection using existing m6A quantitative methods. In this study, we introduced Single-cell Analysis (Scm6A), a machine learning-based approach single-cell quantification. Scm6A leverages input features derived from the expression levels of trans regulators and cis sequence features, offers remarkable prediction efficiency reliability. To further validate...
Background: Patients with COVID-19 often have an impact on populations underlying co-infection. METTL3 plays a crucial role in numerous infectious diseases, making the research broad-spectrum anti-infective drugs targeting both captivating and compelling. Objective: The study aims to identify anti-infection potential compounds using comprehensive virtual screening approach. Method: approach involves ensemble docking molecular dynamics simulations predict validate of natural compounds. This...
VOLUME 288 (2013) PAGES 27059–27067 The interactions between TNFα and the Adalimumab Fab listed in Table 2 references to these data text were incorrect. corrected are provided.TABLE 2Complete list of (≤ 3.65 Å)TNFαAdalimumab FabDistanceResidueAtomResidueAtomCDR...
Abstract It is widely accepted that m 6 A exhibits significant intercellular specificity, which poses challenges for its detection using existing quantitative methods. In this study, we introduce Scm A, a machine learning-based approach single-cell quantification. leverages input features derived from the expression levels of trans regulators and cis sequence features, found offers remarkable prediction efficiency reliability. To further validate robustness precision applied winscore-based...
Abstract As the most abundant mRNA modification in mRNA, N 6 -methyladenosine (m A) plays a crucial role RNA fate, impacting cellular and physiological processes various tumor types. However, our understanding of function m A methylome heterogeneity remains limited. Herein, we collected analyzed methylomes across nine human tissues from 97 A-seq RNA-seq samples. Our findings demonstrate that exhibits different compared to normal tissues, which contributes diverse clinical outcomes cancer We...