A5053873921- Spinal Fractures and Fixation Techniques
- Spine and Intervertebral Disc Pathology
- Pelvic and Acetabular Injuries
- Metabolism and Genetic Disorders
- interferon and immune responses
- Muscle Physiology and Disorders
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Genomics and Rare Diseases
- Cardiomyopathy and Myosin Studies
- Medical Imaging and Analysis
- Bone fractures and treatments
- Cancer Genomics and Diagnostics
- Hip and Femur Fractures
- Genetic factors in colorectal cancer
- Neurogenetic and Muscular Disorders Research
- Toxin Mechanisms and Immunotoxins
- Genomics and Phylogenetic Studies
- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Shoulder and Clavicle Injuries
- Chromosomal and Genetic Variations
- Immune Response and Inflammation
- Microbial Community Ecology and Physiology
Southern University of Science and Technology
2024-2025
Huazhong University of Science and Technology
2019-2025
Second Military Medical University
2012-2025
Changhai Hospital
2023-2025
National Clinical Research Center for Digestive Diseases
2025
Tongji Hospital
2025
National Center of Biomedical Analysis
2018-2024
Northeastern University
2022-2024
First Affiliated Hospital of Soochow University
2024
Soochow University
2024
Hydrogen persulfide and polysulfide (H2Sn) are newly discovered intracellular reactive species considered to have high protein S-sulfhydration efficiency. The detection of H2Sn in living systems is essential for studying their functions but quite challenging. In this work, we report a two-photon excited fluorescent probe, QSn, capable tracking organisms. QSn exhibited turn-on fluorescence response upon reaction with H2Sn. With favorable photophysical property, specificity, low cytotoxicity,...
Abstract Lack of detailed knowledge SARS-CoV-2 infection has been hampering the development treatments for coronavirus disease 2019 (COVID-19). Here, we report that RNA triggers liquid–liquid phase separation (LLPS) nucleocapsid protein, N. By analyzing all 29 proteins SARS-CoV-2, find only N is predicted as an LLPS protein. We further confirm during infection. Among 100,849 genome variants in GISAID database, identify ~37% (36,941) genomes contain a specific trio-nucleotide polymorphism...
ABSTRACT Here we report the crystal structure of hemagglutinin (HA) from influenza B/Hong Kong/8/73 (B/HK) virus determined to 2.8 Å. At a sequence identity ∼25% A HAs, B/HK HA shares similar overall and domain organization. More than two dozen amino acid substitutions on B HAs have been identified cause antigenicity alteration in site-specific mutants, monoclonal antibody escape or field isolates. Mapping these reveals four major epitopes, 120 loop, 150 160 190 helix, that are located...
Receptor-binding specificity of HA, the major surface glycoprotein influenza virus, primarily determines host ranges that virus can infect. Influenza type B almost exclusively infects humans and contributes to annual "flu" sickness. Here we report structures HA in complex with human avian receptor analogs, respectively. These provide a structural basis for different receptor-binding properties A molecules ability distinguish receptors. The structure analog also reveals how mutations region...
Purpose.: The purpose of this study was to establish a fully validated, high-throughput next-generation sequencing (NGS) approach for comprehensive, cost-effective, clinical molecular diagnosis retinitis pigmentosa (RP). Methods.: Target sequences panel 66 genes known cause all nonsyndromic and few syndromic forms RP were enriched by using custom-designed probe hybridization. A total 939 coding exons 20 bp their flanking intron regions with 202,800 target captured, followed massively...
// Guanzhen Yu 1, 2, * , Wenzheng Fang 7, Tian Xia 3, 6, Ying Chen 4, Yunshu Gao 5 Xiaodong Jiao 1 Suyun Huang 2 Jiejun Wang Zhaosheng Li 3 Keping Xie 6 Department of Medical Oncology, Changzheng Hospital, Shanghai 200070, China Neurosurgery, The University Texas MD Anderson Cancer Center, Houston, 77030, USA Gastroenterology, Changhai 200433, 4 Pathology, Qingdao, Shandong 266000, Hepatology & Nutrition, 7 Fuzhou General Clinical College Fujian University, Fuzhou, 350025, These authors have...
The base excision repair glycosylase MUTYH prevents mutations associated with the oxidatively damaged base, 8-oxo-7,8-dihydroguanine (OG), by removing undamaged misincorporated adenines from OG:A mispairs. Defects in individuals inherited variants are correlated colorectal cancer predisposition syndrome known as MUTYH-associated polyposis (MAP). Herein, we reveal key structural features of OG required for efficient human using structure–activity relationships (SAR). We developed a GFP-based...
To establish and evaluate the effectiveness of a comprehensive next-generation sequencing (NGS) approach to simultaneously analyze all genes known be responsible for most clinically genetically heterogeneous neuromuscular diseases (NMDs) involving spinal motoneurons, junctions, nerves, muscles.All coding exons at least 20 bp flanking intronic sequences 236 causing NMDs were enriched by using SeqCap EZ solution-based capture enrichment method followed massively parallel on Illumina...
As an important danger signal, the presence of DNA in cytoplasm triggers potent immune responses. Cyclic GMP-AMP synthase (cGAS) is a recently characterized key sensor for cytoplasmic DNA. The engagement cGAS with leads to synthesis second messenger, cyclic (cGAMP), which binds and activates downstream adaptor protein STING promote type I interferon production. Although has been shown play pivotal role innate immunity, exact regulation activation not fully understood. We report that E3...
Algoriphagus is a heterotrophic bacterium commonly found in diverse marine environments. Here, we report the complete genome sequence of halophilus strain SOCE 003, which 5,154,101 bp long, encoding 5,524 annotated protein-coding genes, 39 tRNAs, and 8 rRNAs. This information will help us understand ecology Algoriphagus.
Understanding the effect of gut microbiota function on immune checkpoint inhibitor (ICI) responses is urgently needed. Here, we integrated 821 fecal metagenomes from 12 datasets to identify differentially abundant genes and construct random forest models predict ICI response. Gene markers demonstrated excellent predictive performance, with an average area under curve (AUC) 0.810. Pathway analyses revealed that quorum sensing (QS), ABC transporters, flagellar assembly, amino acid biosynthesis...
Abstract Mitochondrial dysfunction contributes to the pathogenesis of ulcerative colitis (UC). As a mitochondrial isozyme creatine kinases, which control energy metabolism, CKMT1 is thought be critical molecule in biological processes. However, specific role intestinal inflammation remains largely unknown. Here, we observed markedly decreased expression colon tissues UC patients and dextran sodium sulfate (DSS)-induced mice. We generated epithelial-specific knockout mice demonstrated key...
Libraries of DNA-Encoded small molecules created using combinatorial chemistry and synthetic oligonucleotides are being applied to drug discovery projects across the pharmaceutical industry. The majority reported describe reversible, i.e. non-covalent, target modulators. We synthesized multiple DNA-encoded chemical libraries terminated in electrophiles then used them discover covalent irreversible inhibitors report successful acrylamide- epoxide-terminated Bruton's Tyrosine Kinase (BTK)...
Inspired by the many reported successful applications of DNA-encoded chemical libraries in drug discovery projects with protein targets, we decided to apply this platform nucleic acid targets. We used a 120-billion-compound set 33 distinct and affinity-mediated selection discover binders panel DNA Here, report small molecules that specifically interacted G-quartets, which are stable structural motifs found G-rich regions genomic DNA, including promoter oncogenes. For study, chose G-quartet...
Abstract Emerging evidence demonstrates the clinical utility of genomic applications in newborn intensive care unit (NICU) patients with strong indications Mendelian etiology. However, such applications' diagnostic yield and remain unclear for NICU cohorts minimal phenotype selection. In this study, focused medical exome sequencing was used as a first‐tier, singleton‐focused tool 2303 unrelated sick neonates. Integrated analysis single nucleotide variants (SNVs), small insertions deletions...