A5070868742- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Celiac Disease Research and Management
- Microscopic Colitis
- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Melanoma and MAPK Pathways
- Chronic Lymphocytic Leukemia Research
- Autoimmune and Inflammatory Disorders Research
- Helicobacter pylori-related gastroenterology studies
- Dermatology and Skin Diseases
- PI3K/AKT/mTOR signaling in cancer
- COVID-19 Clinical Research Studies
- Heterotopic Ossification and Related Conditions
- Lymphoma Diagnosis and Treatment
- Adolescent and Pediatric Healthcare
- Inflammatory Myopathies and Dermatomyositis
- COVID-19 and healthcare impacts
- Inflammatory Biomarkers in Disease Prognosis
- T-cell and Retrovirus Studies
- Hair Growth and Disorders
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
Instituto Ramón y Cajal de Investigación Sanitaria
2019-2024
Hospital Universitario Ramón y Cajal
2019-2023
Hospital Clínic de Barcelona
2023
Instituto Cajal
2019-2021
Universidad Autónoma de Madrid
2006-2016
Centro de Biología Molecular Severo Ochoa
2006-2016
Hospital Universitario de La Princesa
2012-2016
Consejo Superior de Investigaciones Científicas
2006-2012
The prevalence of cancer in patients with the novel coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 infection, is uncertain. In a pooled meta-analysis including 11 retrospective studies, was 2%.1 Patients and COVID-19 have been reported to higher risk suffering severe events (intensive care unit admission, invasive ventilation, death) study 18 cancer.2 One 13 cases cohort 128 hospitalised haematological cancers, no significant differences baseline co-variates between developing or...
Folliculitis decalvans (FD) is a rare primary neutrophilic scarring alopecia whose etiology has not been completely elucidated yet.The aim of the study was to determine if follicular microbiota residing in FD-affected hair follicles had distinct microbiological signature and an aberrant immune response present pathogenesis FD.We conducted cross-sectional 10 patients affected by FD. Trichoscopy-guided biopsies were taken from healthy scalp identify microbiome using next-generation sequencing....
Background: Celiac disease is a gluten-related pathology, highly prevalent and heterogeneous in its clinical presentation, which leads to delays diagnosis misdiagnosis. The analysis of duodenal intraepithelial lymphocytes (IELs) by flow cytometry (lymphogram) emerging as discriminative tool the various forms celiac (CD). Aims: aim this study was validate IEL lymphogram performance largest adult series our knowledge, support use diagnostic biomarker dynamic process. Methods: This...
G(q)-coupled G protein-coupled receptors (GPCR) mediate the actions of a variety messengers that are key regulators different cellular functions. These can regulate highly interconnected network biochemical routes control activity several members mitogen-activated protein kinase (MAPK) family. The ERK5 MAPK has been shown to be activated by GPCR via unknown mechanisms. We find atypical C (PKCzeta), previously reported interact with activator MEK5 and involved in epidermal growth...
Gq-coupled G protein-coupled receptors (GPCRs) mediate the actions of a variety messengers that are key regulators cardiovascular function. Enhanced Gαq-mediated signaling plays an important role in cardiac hypertrophy and transition to heart failure. We have recently described Gαq acts as adaptor protein facilitates PKCζ-mediated activation ERK5 epithelial cells. Because cascade is known be involved hypertrophy, we investigated potential relevance this pathway Gq-dependent using both...
G-protein-coupled receptors (GPCRs) are known to activate both G protein- and β-arrestin-dependent signalling cascades. The initiation of mitogen-activated protein kinase (MAPK) pathways is a key downstream event in the control cellular functions including proliferation, differentiation, migration apoptosis. Both proteins β-arrestins have been reported mediate context-specific activation ERK1/2, p38 JNK MAPKs. Recently, ERK5 MAPK by Gq-coupled has described involve direct interaction between...
Heterotrimeric G proteins play an essential role in the initiation of protein-coupled receptor (GPCR) signaling through specific interactions with a variety cellular effectors. We have recently reported that GPCR activation promotes direct interaction between Gαq and protein kinase C ζ (PKCζ), leading to stimulation ERK5 pathway independent canonical effector PLCβ. report herein activation-dependent Gαq/PKCζ complex involves basic PB1-type II domain PKCζ novel module different from classical...
Refractory celiac disease type II (RCD-II) is a very rare yet severe complication of (CD) with 50% rate progression to Enteropathy-associated T-cell lymphoma (EATL). Timely diagnosis and treatment RCD-II the essence requires identification population frequently clonal, phenotypically aberrant intraepithelial lymphocytes (IELs). Flow Cytometry intestinal IELs recommended method identify surface CD3-negative (sCD3-) intracytoplasmic CD3-positive (icCD3ε+) IELs, proportion >20% diagnostic...
INTRODUCTION: Quantitative and phenotypic analyses of duodenal intraepithelial lymphocytes (IELs) by flow cytometry (IEL lymphogram) confer specificity enable the diagnosis even in unconventional presentations celiac disease (CD). To evaluate validity IEL lymphograms pediatric population for new insights into their use as biomarkers natural history CD. METHODS: We retrospectively included 1,211 children (602 with active CD, 92 on a gluten-free diet, 47 potential 470 nonceliac controls) who...
Fecal microbiota transplantation (FMT) is an effective procedure against Clostridioides difficile infection (CDI), with promising but still suboptimal performance in other diseases, such as ulcerative colitis (UC). The recipient’s mucosal immune response the donor’s could be relevant factor effectiveness of FMT. Our aim was to design and validate individualized immune-based test optimize fecal donor selection for First, we performed vitro validation by co-culturing lymphocytes obtained from...
Background: TNF inhibitors (TNFis) are widely used for the treatment of rheumatoid arthritis (RA), although response rates to this therapy in patients with RA remains heterogeneous and less than 50% achieve remission (REM). Objective: To analyze baseline peripheral blood leukocytes profiles order search biomarkers identifying who will most likely not REM under TNFi treatment. Methods: A prospective bi-center pilot study including 98 treated TNFis followed-up during 6 months. Patients were...
<h3>Background</h3> Rheumatoid arthritis (RA) is a chronic inflammatory disease with the typical characteristic of synovitis small-sized and medium-sized joints that leads to cartilage bone damage. TNF inhibitors (TNFi) are widely used for treatment rheumatoid however, there still no objective indicators clinical response TNFi therapy. <h3>Objectives</h3> To analyse change peripheral blood mononuclear cells (PBMC) profile after 6 months (m) in order find cellular response. <h3>Methods</h3>...
Background: Idiopathic inflammatory myopathies (IIM) are a group of immune-mediated diseases characterized my muscle weakness, skin rash and systemic involvement. Myositis-specific antibodies (MSA) myositis-associated (MAA) play major role in IIM diagnosis, classification prognosis. Nevertheless, MSA/MAA testing is not standardized there very few studies addressing their relationship with other diseases. Objectives: To describe cohort patients tested positive for MSA/MAA, to explore it´s...
Biological therapies, such as TNF inhibitors (TNFi), are increasing remission (REM) rates in rheumatoid arthritis (RA) patients, although these still limited. The aim of our study was to analyze changes the profile peripheral blood mononuclear cells (PBMC) patients with RA treated TNFi relation clinical response. This is a prospective and observational including 78 starting first TNFi. PBMC were analyzed by flow cytometry both at baseline 6 months. Disease activity same time points assessed...
<h3>Background:</h3> Systemic Juvenile Idiopathic Arthritis (SJIA) and adult onset Still's disease are rare diseases of unknown etiology that share several clinical laboratory features. The pathogenesis these two is complex multifactorial, with an important role innate immunity: activation neutrophils macrophages increased levels cytokines dependent on the inflammosome. A proportion patients will develop a Macrophage syndrome (MAS), serious life-threatening complication. <h3>Objectives:</h3>...
<h3>Background:</h3> The most common biological agents used as disease-modifying treatment in rheumatoid arthritis (RA) are TNF inhibitors (TNFi). Although these new strategies to treat RA have improved the course of disease, approximately 30-50% patients do not respond this therapy. Early identification optimal responders is crucial clinical setting. <h3>Objectives:</h3> aim study was investigate if baseline percentages different leukocyte subsets peripheral blood (PBMCs) can contribute...
Background: According to the EULAR recommendations, therapeutic target in patients with RA should be remission (REM). However, no more than 50% of treated TNF inhibitors (TNFi) attains this outcome. Previous investigations suggested peripheral blood mononuclear cells (PBMC) as markers associated TNFi treatment success 1,2 . Granulocyte-monocyte colony-stimulating factor (GM-CSF) plays a relevant role pathogenesis rheumatoid arthritis (RA) because it promotes macrophage differentiation,...