Background With increasing knowledge about the gut – bone axis, more studies for treatments based on regulation of postmenopausal osteoporosis by microbes are being conducted. Based our previous work, this study was conducted to further investigate therapeutic effects Lactobacillus rhamnosus GG (LGG) ovariectomized (OVX) model rats and immunological microecological mechanisms involved.Results We found a protective effect LGG treatment in OVX through changes microarchitecture, biomechanics,...

The processing of traditional Chinese medicine (TCM) is a unique pharmaceutical technology in China, which the most important feature that distinguishes from natural and plant medicine. Since record Huangdi Neijing (Inner Canon Yellow Emperor), till now, TCM has experienced more than 2000 years inheritance, innovation, development, combination theory clinical practice, plays an extremely position field TCM. In recent years, as prescription TCM, herbal pieces have played significant role...

Rhubarb is one of the most popular traditional Chinese medicines and has been used for thousands years in many Asian countries. Prepared rhubarb obtained by steaming raw with glutinous rice wine until it turned black appearance both inside outside. After processing, therapeutic effects prepared change a lot. To find out exact compound changes chemical profile decoction after processing to clarify material basis changed effects, an ultra-high performance liquid chromatography quadrupole...

In this study, it is the first that Viticis Fructus (VF) was used as raw material for extracting total flavonoids using ultrasound-assisted enzyme extraction (UAE) method. Response surface methodology employed to determine optimal parameters. The conditions were follows: 60 % ethanol solution extract solvent, material-liquid ratio of 1:25, pH value 4, addition amount 1.5 %, enzymatic hydrolysis time 30 min, temperature 40 ℃, and ultrasonic 50 min. Comparing flavonoid yield VF processed (PVF)...

ABSTRACT Choerospondias axillaris is a medicinal plant used for treating coronary heart disease (CHD) due to its broad spectrum of anti‐inflammatory activities. Cyclooxygenase 2 (COX‐2) and lipoxygenase 5 (5‐LOX) were immobilized on magnetic nanoparticles selective ligand‐extraction these two enzymes present in C. . Sixteen extracted components identified analyzed using ultraperformance liquid chromatography plus Q‐Exactive Orbitrap tandem mass spectrometry (UHPLC‐Q‐ExactiveOrbitrap‐MS/MS),...

Isoliquiritigenin (ISL) is an abundant dietary flavonoid with numerous pharmacological activities. However, isoliquiritigenin poorly absorbed by the body which limits its clinical usage. Cocrystallization has attracted attention recently as a means of modifying unfavourable physicochemical properties compounds. Herein, we report synthesis two new cocrystals ISL: isoliquiritigenin–nicotinamide (ISL–NIC) and isoliquiritigenin–isonicotinamide (ISL–INM). To our knowledge, this first from...

The sulfur-fumigation process can induce changes in the contents of volatile compounds and chemical transformation herbal medicines. Although literature has reported many methods for analyzing target from medicine, all them are largely limited to sun-dried samples. This study provides a comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC×GC-TOF/MS) method based on profiling approach identify non-target sulfur-fumigated medicine. Using Chrysanthemum...

Flos Lonicerae Japonicae (FLJ) is a popular herb used for many centuries in Traditional Chinese Medicine as treatment of fever and inflammation. Non-fumigated processing FLJ has been the traditional approach post-harvest preparation commodity commercial use. However, recent years, natural drying replaced by sulfur-fumigation efficiency pest control. Sulfur-fumigation can induce changes volatile compounds herb, altering its medicinal properties. A comprehensive two-dimensional gas...

Pericarpium Citri Reticulatae (Chenpi in Chinese) has been widely used as an herbal medicine Korea, China, and Japan. Chenpi extracts are to treat indigestion inflammatory syndromes of the respiratory tract such bronchitis asthma. This thesis will analyze chemical compositions volatile oil, which was performed by comprehensive two-dimensional gas chromatography with high-resolution time-of-flight mass spectrometry (GC × GC-HR-TOFMS). One hundred sixty-seven components were tentatively...

In traditional Chinese medicine, raw and processed herbs are used to treat different diseases. Suitable quality assessment methods crucial for the discrimination between herbs. The dried fruit of Arctium lappa L. their products widely in yet therapeutic effects different. this study, a novel strategy using high-performance liquid chromatography diode array detection coupled with multivariate statistical analysis rapidly explore was proposed validated. Four main components total 30 batches...

In China, Semen Cassiae has long been used to protect liver, brighten eyes, and relieve constipation. Prepared is produced from raw by processing, the two forms of have different clinical applications. Pathological state an important factor affecting efficacy drugs, pharmacokinetic behavior drugs could be significantly changed when people or animal were under pathological state. To clarify effect processing mechanism for behavior, pharmacokinetics nine components prepared normal acute liver...

The clinical use of doxorubicin (DOX) is severely limited due to its cardiotoxicity. Thus, there a need for prophylactic and treatment strategies against DOX-induced cardiotoxicity.The purpose this study was develop liquiritigenin-loaded submicron emulsion (Lq-SE) with enhanced oral bioavailability explore efficacy cardiotoxicity.Lq-SE prepared using high-pressure homogenization characterized several analytical techniques. formulation optimized by central composite design response surface...

ABSTRACT A rapid and sensitive high‐performance liquid chromatography–mass spectrometric (HPLC‐MS) method was developed validated for simultaneous determination of benzoylhypaconine (BHA), benzoylmesaconine (BMA), benzoylaconine (BAC) hypaconitine (HA) in rat plasma the first time. The analytes were separated on a Kromasil C 18 column with total running time 11 min. validation data demonstrated sound feasibility newly it then applied to pharmacokinetic study these rats. Pharmacokinetic...

Cimicifugae Rhizoma (Sheng ma) is a Ranunculaceae herb belonging to composite family and well known in China. has been widely used traditional Chinese medicine. The Pharmacopoeia of the People׳s Republic China contains three varieties (Cimicifuga dahurica (Turcz.), Cimicifuga foetida L. heracleifolia Kom.) which have clinically as "Sheng-ma". However, chemical constituents components "Sheng-ma" never documented. In this study, rapid method for analysis main was developed using ultra-high...

Sulfur-fumigation may induce chemical transformation of traditional Chinese medicines leading to harmful effects following patient ingestion. For quality control, it is urgently needed develop a reliable and efficient method for sulfur-fumigation identification.The spectrochemical identification non-fumigated sulfur-fumigated Fritillaria thunbergii Miq. was carried out evaluate inorganic elements organic components. The concentrations 12 elements, including Zn, Mn, Cu, Fe, Li, Mg, Sr, Pb,...

Pharmacognosy Magazine,2012,8,32,300-307.DOI:10.4103/0973-1296.103659Published:November 2012Type:Original ArticleAuthors:Hao Cai, Zhiwei Xu, Sucai Luo, Wenwen Zhang, Gang Cao, Xiao Liu, Yajing Lou, Xiaoqing Ma, Kunming Qin, and Baochang Cai Author(s) affiliations:Hao Cai1, Xu2, Luo2, Zhang2, Cao2, Liu1, Lou3, Ma3, Qin3, Cai4 1Department of Chinese Materia Medica, College Pharmacy, Nanjing University Medicine, - 210046; Engineering Center State Ministry Education for Standardization Medicine...

In traditional Chinese medicine (TCM), raw and processed herbs are used to treat the different diseases. Fructus Arctii, dried fruits of Arctium lappa l. (Compositae), is widely in TCM. Stir-frying most common processing method, which might modify chemical compositions Arctii.To test this hypothesis, we focused on analysis identification main constituents Arctii (PFA) by high-performance liquid chromatography/diode array detection-electrospray ionization-mass spectrometry.The results...