Although Traditional Chinese Medicine (TCM) preparations have long history with successful applications, the scientific and systematic quality assessment of TCM mainly focuses on chemical constituents is far from comprehensive. There are currently only few primitive studies biological ingredients in preparations. Here, we proposed a method, M-TCM, for based high-throughput sequencing metagenomic analysis. We tested this method Liuwei Dihuang Wan (LDW), whose been well-defined. Our results...

Abstract: Rheumatoid arthritis (RA) is an autoimmune disease characterized by progressive cartilage and bone destruction. Activated macrophages that overexpress folic acid (FA) receptors play important role in RA, due to their abundance inflamed synovial membrane joints. In effort deliver drugs the tissues, multifunctional FA receptor-targeting pH-responsive nanocarriers were developed. They composed of lipids, polyethylene glycol (PEG)–poly(lactic- co -glycolic acid) (PLGA) forming a...

To develop green finance and ensure the goal of carbon peaking neutrality, China set up pilot zones for reform innovation in 2017. We empirically tested policy effect with data from 2010 to 2019 prefecture-level cities China. The study shows that have induced an on regional technology innovation, reflected application acquisition both invention patents utility patents, promotion is better than which supported by robustness test using PSM-DID. This provides theoretical support empirical...

Ginseng (Panax ginseng C. A. Mey) is a common traditional Chinese medicine used for anti-inflammation, treating colitis, type 2 diabetes, diarrhea, and recovering hepatobiliary function. Ginsenosides, the main active components isolated from ginseng, possess liver gallbladder diseases therapeutic potential. Cholestatic injury (CLI) disease induced by intrahepatic accumulation of toxic bile acids currently lacks clinically effective drugs. Our previous study found that ginsenosides alleviated...

Abstract Skin fibrosis is characterized by the excessive accumulation of extracellular matrix (ECM) caused fibrotic disorders skin. In recent years, ECM stiffness has emerged as a prominent mechanical cue that precedes skin and drives its progression promoting fibroblasts activation. However, how influences activation for remains unknown. Here, we report positive feedback loop mediated mechanosensitive ion channel Piezo1 aberrant tissue mechanics in driving fibrosis. upregulated both humans...

MicroRNA-21 (miR-21) is an oncomiR that frequently upregulated in human cancers. AntimiR-21 (AM-21) oligonucleotide complementary to miR-21 designed inhibit its gene silencing activities. To facilitate efficient delivery of AM-21, a novel lipid nanoparticle formulation called QTsome, based on combination quaternary amine and tertiary cationic lipids, with distinctive pH-responsive profile, was developed. QTsome/AM-21 comprising DODMA/DOTAP/DOPC/CHOL/mPEG-DPPE AM-21 exhibited mean particle...

In this research, a multi-input multi-output artificial neural network (MIMO-ANN) is developed, in which 14 features associated with the engine performance and stability, emissions, combustion ignition characteristics of diesel-biodiesel-gasoline mixtures are meant to be modelled by diverse combination engine/combustion parameters. The selected targets comprise brake specific fuel consumption (BSFC), thermal efficiency (BTE), efficiency, coefficient variance (COV), NOx, CO2, CO HC exhaust...

Hepatocellular carcinoma (HCC) is one of the most common cancers, with high mortality. Chemotherapy main treatment options for HCC. However, toxicity and poor specificity chemotherapeutic drugs have limited their clinical application. In this study, dual-ligand liposomes modified glycyrrhetinic acid (GA) cyclic arginine–glycine–aspartic (cRGD) (GA/cRGD-LP) were designed to target GA receptor αvβ3 integrin, respectively. The aim was develop a highly selective targeted drug delivery system...

Tacrolimus-induced chronic nephrotoxicity (TICN) hinders its long-term use, but mechanism remains unclear. Tacrolimus exerts pharmacological effect by inhibiting calcineurin and substrate NFAT. Whether the inhibition of other substrates is related to TICN be explored. Transcription factor EB (TFEB), a calcineurin, plays crucial role in various homeostasis. Herein, we found that tacrolimus inhibited TFEB nuclear translocation activity mouse kidneys HK-2 cells. Then, gain- loss-of-function...

Estrogens have been reported to cause dysfunction in biliary transport systems, thereby inducing cholestasis. Multidrug resistance-associated protein 2 (MRP2) is a transporter responsible for independent bile flow. Emerging evidence indicates that PDZ domain containing 1 (PDZK1) regulates localization of MRP2; however, PDZK1’s role and regulatory machinery MRP2-mediated estrogen-induced cholestasis (EIC) remain unclear. Herein, mouse model EIC, we observed downregulated PDZK1 expression the...

Expression of Bcl-2 and Akt-1 has been associated with human cancer. G3139 RX-0201, targeting Akt-1, respectively, are antisense oligonucleotides (ASOs) that have shown limited efficacy in clinical trials. Herein, we report a combination newly designed ASOs based on these agents was delivered by tumor cell-targeting lipid nanoparticles (LNPs). A "Gapmer" design strategy applied to the addition 2'-O-methyl modifications nucleotides at 5' 3' ends. dual-channel syringe pump-based system...

Camptothecin (CPT), a natural DNA topoisomerase 1 inhibitor, is commonly used as model hydrophobic compound for anticancer drug delivery research. Nanoparticle loaded with active drugs has the potential to selectively deliver therapeutic agents into tumor cells without affecting normal tissues, and hence attracting tremendous attention from biomedical scientists. Nanoparticles can benefit enhanced permeability retention effect (EPR) of tissues that allows nanoparticles be transported through...

This paper reports the development, validation, and application of thermophysical transport properties coconut, palm, soy methyl esters for fuel spray combustion modeling under light-duty diesel engine conditions. The developed library is implemented in an open-source CFD code. are validated both constant volume chamber compression ignition (CI) operation at a wide range Sensitivity analysis on effects individual also investigated interest study density, vapor pressure, heat vaporization,...

In this research, the spray combustion, soot formation and exhaust emissions of diesel, biodiesel, gasoline fuels their mixtures are analysed in a constant volume chamber. A multicomponent kinetic mechanism (CDBG) suitable for diesel-biodiesel-gasoline developed by our research group is utilised, associated physicochemical properties thoroughly calculated. Adaptive mesh refinement scheme with appropriate independency analysis applied. Liquid penetration length, lift-off ignition delay have...

Biodiesel combustion in diesel engines is associated with reduced carbon monoxide, particulate matter and unburned hydrocarbons emissions, but several concerns remain including deposition, low thermal efficiency elevated nitrogen oxides production. To this end, the incorporation of gasoline additive has been proposed as a solution to drawbacks posed by biodiesel. Integrated chemical kinetics-computational fluid dynamics modelling widely used tool understand characteristics new fuel mixtures....

Lipid-albumin nanoparticles (LAN) were synthesized for delivery of RX-0047, an antisense oligonucleotide (ASO) against the hypoxia inducible factor-1 alpha (HIF-1α) to solid tumor. These lipid (LNs) incorporated a human serum albumin-pentaethylenehexamine (HSA-PEHA) conjugate, which is cationic and can form electrostatic complexes with negatively charged oligonucleotides. The efficiency LAN-RX-0047 was investigated in KB cells murine xenograft model. When treated LAN-RX-0047, significant...

Abstract Traditional Chinese medicine (TCM) preparations are widely used for healthcare and clinical practice. So far, the methods commonly quality evaluation of TCM mainly focused on chemical ingredients. The biological ingredient analysis is also important because usually contain both plant animal ingredients, which often include some mis-identified herbal materials, adulterants or even contaminants. For analysis, efficiency DNA extraction an factor might affect accuracy reliability...