A5086445793- Reproductive tract infections research
- Natural product bioactivities and synthesis
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Traditional Chinese Medicine Analysis
- Phytochemical Studies and Bioactivities
- Phytochemistry and Bioactive Compounds
- Traditional Chinese Medicine Studies
- Metabolomics and Mass Spectrometry Studies
- Phytochemistry and Biological Activities
- Biological Stains and Phytochemicals
- Dermatology and Skin Diseases
- Plant biochemistry and biosynthesis
- Phytochemistry and biological activities of Ficus species
- Phytochemistry and Bioactivity Studies
- HVDC Systems and Fault Protection
- Cervical Cancer and HPV Research
- Power Systems and Renewable Energy
- Integrated Energy Systems Optimization
- Renal cell carcinoma treatment
- Neurological Disease Mechanisms and Treatments
- T-cell and B-cell Immunology
- Ferroptosis and cancer prognosis
Tianjin Medical University
2009-2025
South China Normal University
2024
China Electric Power Research Institute
2014-2023
North China Electric Power University
2014-2023
Huazhong University of Science and Technology
2016-2022
National Medical Products Administration
2007-2022
Zhejiang Provincial People's Hospital
2012-2021
Hangzhou Medical College
2021
Dalian Maternal and Child Health Hospital
2021
Dalian Medical University
2021
Abstract Although their contribution to host defense against extracellular infections has been well defined, IL-17 and Th17 are generally thought have limited impact on intracellular infections. In this study, we investigated the role mechanisms of IL-17/Th17 in Chlamydia muridarum, an obligate bacterium, lung infection. Our data showed rapid increase production expansion cells following C. muridarum infection significant detrimental vivo neutralization by anti-IL-17 mAb disease course,...
Although Traditional Chinese Medicine (TCM) preparations have long history with successful applications, the scientific and systematic quality assessment of TCM mainly focuses on chemical constituents is far from comprehensive. There are currently only few primitive studies biological ingredients in preparations. Here, we proposed a method, M-TCM, for based high-throughput sequencing metagenomic analysis. We tested this method Liuwei Dihuang Wan (LDW), whose been well-defined. Our results...
CCR2, a member of the G protein-coupled receptor (GPCR) superfamily, is widely expressed on monocytes, macrophages, activated T cells, and other cell types, plays critical role in coordinating immune response to various infections. Here we demonstrate that CCR2 expression significantly elevated during Chlamydia muridarum ( C . ) respiratory infection, its absence leads exacerbated susceptibility, as evidenced by significant weight loss, higher bacterial loads, severe lung pathology, levels...
Abstract Type I IFNs (IFNIs) have pleiotropic functions in regulating host innate and adaptive immune responses to pathogens. To elucidate the role of IFNIs resistance chlamydial infection vivo, we compared IFN-α/β receptor knockout (IFNAR−/−) wild-type control mice susceptibility Chlamydia trachomatis mouse pneumonitis (Chlamydia muridarum) lung infection. We found that IFNAR−/− were significantly more resistant C. muridarum showing less bacterial burden bodyweight loss, milder pathological...
Ten new triterpenoid saponins (1-10), named rubusides A-J, and 21 known (11-31) were isolated from the roots of Rubus ellipticus var. obcordatus. The structures 1-10 established on basis spectroscopic analyses, mainly NMR MS, chemical degradations. compounds demonstrated inhibitory activities against alpha-glucosidase with IC50 values in range 0.65-3.09 mM.
IFN-γ-mediated inducible nitric oxide synthase (iNOS) expression is critical for controlling chlamydial infection through microbicidal (NO) production. Interleukin-17A (IL-17A), as a new proinflammatory cytokine, has been shown to play protective role in host defense against Chlamydia muridarum (Cm) infection. To define the related mechanism, we investigated, present study, effect of IL-17A on IFN-γ induced iNOS and NO production during Cm vitro vivo. Our data showed that significantly...
Abstract The product template (PT) domains, specifically in fungal non-reducing polyketide synthases (NR-PKSs), mediate the regioselective cyclization of polyketides dominating final structures. However, up to date, systematic knowledge about PT domains has been insufficient. In present study, relationships between sequences, structures and functions were analyzed with 661 NR-PKS sequences. Based on phylogenetic analysis, classified into prominent eight groups (I–VIII) corresponding...
Abstract With the rapid development of high-throughput sequencing technology, approaches for assessing biological ingredients in Traditional Chinese Medicine (TCM) preparations have also advanced. Using a multi-barcode approach, all could be identified from TCM theory, as long their DNA is present. The several classical were analyzed successfully based on this approach previous studies. However, universality, sensitivity and reliability diverse set remain unclear. In study, we selected four...
Five new monoterpene glycosides, rhodiolosides A-E (1-5), were isolated from the roots of Rhodiola rosea (Crassulaceae). Their structures elucidated as (2E,6E,4R)-4,8-dihydroxy-3,7-dimethyl-2,6-octadienyl beta-D-glucopyranoside (1), (2E,4R)-4-hydroxy-3,7-dimethyl-2,6-octadienyl alpha-D-glucopyranosyl(1-->6)-beta-D-glucopyranoside (2), beta-D-glucopyranosyl(1-->3)-beta-D-glucopyranoside (3), (2E,4R)-4,7-dihydroxy-3,7-dimethyl-2-octenyl (4), and (2E)-7-hydroxy-3,7-dimethyl-2-octenyl...
The impact of the interaction between NK cells and lung dendritic (LDCs) on outcome respiratory infections is poorly understood. In this study, we investigated effect mechanism function LDCs during intracellular bacterial infection Chlamydia muridarum in mice. We found that naive mice receiving from C. ‐infected NK‐cell‐depleted (NK‐LDCs) showed more serious body weight loss, burden, pathology upon chlamydial challenge when compared with recipients infected sham‐treated (NK+LDCs). Cytokine...
Abstract Traditional Chinese medicine (TCM) preparations are widely used for healthcare and clinical practice. So far, the methods commonly quality evaluation of TCM mainly focused on chemical ingredients. The biological ingredient analysis is also important because usually contain both plant animal ingredients, which often include some mis-identified herbal materials, adulterants or even contaminants. For analysis, efficiency DNA extraction an factor might affect accuracy reliability...
The rhizomes of Polygonatum odoratum represent a traditional Chinese medicine and functional food. A phytochemical investigation resulted in the isolation eight steroidal glycosides (1–8), including two new compounds, polygonatumosides F (1) G (2). structures were elucidated by spectroscopic data chemical reactions. Compound 7 showed antiproliferation activity against human hepatocellular carcinoma cell line HepG2 (IC50 3.2 μM). profile contents P. collected at different dates geographical...
IL-27, a heterodimeric cytokine of the IL-12 family, has diverse influences on development multiple inflammatory diseases. In this study, we identified protective role IL-27/IL-27R in host defense against Chlamydia muridarum respiratory infection and further investigated immunological mechanism. Our results showed that IL-27 was involved C. IL-27R knockout mice (WSX-1-/- mice) suffered more severe disease, with greater body weight loss, higher chlamydial loads, reactions lungs than C57BL/6...