A5040177256- Effects of Radiation Exposure
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Radioactive contamination and transfer
- Radiation Therapy and Dosimetry
- Cancer-related molecular mechanisms research
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Chemical Reactions and Isotopes
- Radioactivity and Radon Measurements
- Cell death mechanisms and regulation
- Renal and related cancers
- Cancer-related Molecular Pathways
- Glioma Diagnosis and Treatment
- Oral health in cancer treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer, Stress, Anesthesia, and Immune Response
- Neurological Disease Mechanisms and Treatments
- Occupational and environmental lung diseases
- Wound Healing and Treatments
- Brain Metastases and Treatment
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
Soochow University
2016-2025
University of Alabama at Birmingham
2016-2020
Beijing Radiation Center
2013
Zero to Three
2013
This study investigated whether exosomal microRNA-7 (miR-7) mediates lung bystander autophagy after focal brain irradiation in mice. After 10 Gy or sham of mice brains, tissues were extracted for the detection markers by immunohistochemistry, western blotting, and quantitative real-time reverse transcription PCR (qRT-PCR), meanwhile brains dissociated, neuron/astrocyte/microglia/oligodendrocyte isolated, miR-7 expression each population detected, respectively. A dual-luciferase reporter...
ABSTRACT As immune cells, neutrophils serve as the first line of defense against infections; however, mechanism by which regulate lipid metabolism is unknown. The neutrophil depletion group was treated with 100 μg InVivoMAb anti‐mouse Ly6G 6 times, whereas control mice were intraperitoneally injected same quantity rat IgG2a. Body fat content, triglycerides (TGs), total cholesterol (TC), low‐density lipoprotein (LDL‐C) in jejunum and ileum, well 9 long‐chain fatty acids (LCFAs) intestinal...
Radon and its progeny are confirmed to be type I carcinogenic agents accounting for increased risks in 10% of observed lung cancers globally. However, the underlying mechanisms largely unknown. In present study, BEAS2B cells were directly exposed twice 20,000 Bq/m3 radon gas 20 min once (first passage) subsequently 10 times (fifth passage). The fifth-passage then subcultured 1 generations (named Rn5-1 Rn5-20, respectively). Molecular indicative malignant transformation assessed by...
Long non‑coding RNAs (lncRNAs) have been regarded as the primary genetic regulators of several important biological processes. However, functions lncRNAs in radiation‑induced lung damage remain largely unknown. The present study aimed to investigate potential effects on injury (RILI). Female C57BL/6 mice were exposed 12 Gy single doses total body irradiation (TBI). LncRNA microarray screening was conducted at 24 h post‑irradiation (IR) differentially‑expressed during RILI. Following...
Liver kinase B1 (LKB1) functions as a tumor suppressor encoded by STK11, gene that mutated in Peutz-Jeghers syndrome and sporadic cancers. Previous studies showed LKB1 participates IR- ROS-induced DNA damage response (DDR). However, the impact of mutations on targeted cancer therapy remains unknown. Herein, we demonstrated formed damage-induced nuclear foci co-localized with ataxia telangiectasia (ATM), γ-H2AX, breast susceptibility 1 (BRCA1). ATM mediated phosphorylation at Thr 363...
Background: Quinacrine (QC), an antimalarial drug, has been shown to possess anticancer effect both in vitro (cancer cell lines) and vivo (mouse models).In the cancer cells, QC can simultaneously suppress nuclear factor-B activate p53 signaling, which results induction of apoptosis these cells.However, experimental come from a few limited lines, detailed mechanisms remain unknown.Objective: This study investigated tumor-killing effects on gastric cells as well underlying molecular...
The identification of an agent effective for the treatment intestinal and bone marrow injury following radiation exposure remains a major issue in radiological medicine. In this study, we evaluated therapeutic impact single or combination treatments with 2-(3-aminopropylamino) ethylsulphanyl phosphonic acid (WR-2721) peptidoglycan (PGN, toll-like receptor 2 (TLR-2) agonist) on radiation-induced intestine lethally irradiated male C57BL/6 mice. A dose 3 mg WR-2721 per mouse (167 mg/kg,...
Abstract Aim The study was conducted to investigate the role of c‐Myc in regulation ionizing radiation‐induced cell cycle arrest and death human cervical cancer cells. Methods Control c‐Myc‐silenced Hela cells were collected at different time points after 60 Co γ‐ray radiation. Flow cytometry used measure distribution apoptosis. Immunofluorescence applied determine percentage M phase. Transmission electron microscopy immunoblotting detect induction autophagy Immunoblotting also expression...
This study aimed to examine the radioprotective effect of polydatin (PD) on crypt and endothelial cells small intestines C57BL/6 mice that received abdominal irradiation (IR). Mice were treated with 6 MV X-ray (20 Gy) IR at a dose rate 200 cGy/min. Thirty minutes before or after IR, intraperitoneally injected PD. The survival 30 days was determined. duodenum (upper intestine), jejunum (middle ileum (lower intestine) collected subjected hematoxylin eosin staining. Tissue sample sections...
Non-obstructive azoospermia (NOA) is a common cause of male infertility, and no specific diagnostic indicators exist. In this study, we used human testis datasets GSE45885, GSE45887, GSE108886 from GEO database as training datasets, screened 6 signature genes (all lowly expressed in the NOA group) using Boruta algorithm Lasso regression: C12orf54, TSSK6, OR2H1, FER1L5, C9orf153, XKR3. The efficacy above was examined by constructing models with LightGBM algorithm: AUC (Area Under Curve) both...
The aim of the study is to investigate radioprotective effect polysaccharide extract from Sipunculus nudus (SNP). Beagle dogs were randomly divided into following six groups. Group-1: Un-treated and un-irradiated controls. Group-2: Exposed a single acute dose 2 Gy γ-radiation alone. Groups-3, 4 5: Oral administration SNP at 50, 100 or 200 mg/kg body weight once day for 7 days followed by whole exposure γ-radiation. same doses administered further 27 days. Group-6: Positive controls treated...
The effects of ionizing radiation on the reproductive system have always been a matter great interest. Both artificial and naturally occurring can directly or indirectly affect via introduction DNA single-strand double-strand breaks, excitation water molecules, generation free radicals. In order to quantitatively investigate function, 60Co γ irradiation was applied model organism, Caenorhabditis elegans (C. elegans). egg-laying embryo-hatching activities were observed for parent (F0) first 2...
This study aimed to develop an exosome-coated polydatin (PD) nanoparticles (exo-PD) for improving the water solubility and bioavailability of explore its salutary effects on intestinal radiation injury. Exosomes (exo) were extracted from medium human amniotic fluid stem cells (hAFSc). Mice divided into control group, irradiation (IR) irradiation+PD (IR+PD) irradiation+exo (IR+exo) group irradiation+exo-PD (IR+exo-PD) group. The results characterization protein markers, particle size,...
Tritium is a water-soluble hydrogen isotope that releases beta rays during decay. In nature, tritium primarily exists as tritiated water (HTO), and its main source nuclear power/processing plants. recent decades, with the development of power industry, it necessary to evaluate impact on organisms. this study, fertilized zebrafish embryos are treated different HTO concentrations (3.7 × 103 Bq/ml, 3.7 104 105 Bq/ml). After treatment HTO, developed without evident morphological changes....
Griseofulvin, an oral nontoxic antifungal drug, has been reported to possess anticancer effect in human cancer cells, while the mechanisms are not completely understood.The aim of this study was investigate cytotoxic griseofulvin on K562 cells and understand its underlying molecular pathways.K562 were treated with at different concentrations for 24 hours, inhibition cell proliferation assessed by tetrazolium salt colorimetric assay. Apoptosis examining nuclear morphology quantifying...
Enterocytes die during high-dose radiation exposure in accidents. The modality of cell death has a profound effect on the therapeutic response. ilea from mice with 15 Gy total body irradiation (TBI) were drawn, morphological features observed by hematoxylin and eosin staining transmission electron micrographs. biochemical mouse ileum presented structure cleaved Caspase-3 (apoptosis marker), Light Chain 3 (LC3)-I's conversion to LC3-II (autophagy marker) high mobility group box chromosomal...
We aimed to determine the toxic effects of tritiated water (HTO) on 12 generations (T1-T12) human umbilical vein vascular endothelial cells (HUVECs) and elucidate underlying mechanisms. evaluated cellular senescence, interleukin (IL) 8 concentrations, angiogenesis using β-galactosidase staining, enzyme-linked immunosorbent assay, in vitro assays, respectively. The adhesion properties contaminated differentially expressed genes were assessed xCELLigence RTCA SP system gene chip analysis,...
Abstract Designing skin decontaminating materials with outstanding therapeutic effects, adhesiveness, and suitable mechanical property has great practical significance in radionuclide‐contaminated wound healing. Here, a physically crosslinked hydrogel is constructed via hydrogen bonding of poly acrylamide, sodium alginate (SA), the complexing agent diethylene triamine pentaacetic acid (DTPA). The physical chemical properties poly(AAm‐SA‐DTPA) (PASD) are detected according to established...