A5100632515- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Cancer Cells and Metastasis
- Cardiac Ischemia and Reperfusion
- Diet, Metabolism, and Disease
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Nitrogen and Sulfur Effects on Brassica
- Fibroblast Growth Factor Research
- Head and Neck Cancer Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Lipids, and Metabolism
- Pluripotent Stem Cells Research
- TGF-β signaling in diseases
Second Affiliated Hospital of Harbin Medical University
2015-2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Zhongshan Hospital
2023
Fudan University
2021-2023
Sun Yat-sen University
2023
Harbin Medical University
2015-2023
Heilongjiang Center for Tuberculosis Control and Prevention
2021
Zhejiang Hospital
2021
People's Liberation Army 401 Hospital
2020
Chinese People's Liberation Army
2020
A growing body of evidence has indicated that long non-coding RNAs (lncRNAs) serve as competing endogenous (ceRNAs) during oncogenesis. In this study, the qRT-PCR results lncRNA PVT1 is overexpressed in osteosarcoma and decreased survival rate patients. MTT clonal colony formation assays were used to detect effect on proliferation, flow cytometry was performed assess apoptosis cell cycle. Transwell assay analyze migration invasion. The revealed silencing by siRNA inhibited invasion promoted...
Abstract Hepatic insulin resistance is central to the metabolic syndrome. Here we investigate role of BTB and CNC homology 1 (BACH1) in hepatic signaling. BACH1 elevated hepatocytes individuals with obesity patients non-alcoholic fatty liver disease (NAFLD). Hepatocyte-specific Bach1 deletion male mice on a high-fat diet (HFD) ameliorates hyperglycemia resistance, improves glucose homeostasis, protects against steatosis, whereas overexpression leads opposite phenotype. directly interacts...
Maintenance of stem-cell identity requires proper regulation enhancer activity. Both transcription factors OCT4/SOX2/NANOG and histone methyltransferase complexes MLL/SET1 were shown to regulate activity, but how they are regulated in embryonic stem cells (ESCs) remains further studies. Here, we report a factor BACH1, which directly interacts with (OSN) maintains pluripotency mouse ESCs (mESCs). BTB domain bZIP BACH1 required for these interactions maintenance. Loss reduced the interaction...
Estrogens are involved in the complex regulation of cell proliferation and apoptosis hormone sensitive tumors including breast endometrial cancers. Sulfation is main pathway for estrogen metabolism, which believed to be inactivation estrogens target tissues. SULT 1 E PAPSS ( 2) responsible sulfation by providing catalyzing enzyme universal sulfate donor. The present study showed expression patterns tissues tissue array analysis assessment clinical samples. enzymes were comparatively higher...
Long non-coding RNAs (lncRNAs) have emerged as key regulators of Toll-like receptor (TLR) signaling to control innate immunity, and this regulatory mechanism has recently been implicated in esophageal carcinoma (ESCA). However, a comprehensive analysis TLR-induced lncRNAs their roles diagnosis prognosis ESCA is still lacking. In study, we first investigated the precise relationship between lncRNA perturbations alteration TLR by constructing lncRNA-TLRs co-expression network involved ESCA,...
AIMTo investigate the functional role and underlying molecular mechanism of miR-29a in hepatitis B virus (HBV) expression replication. METHODSThe levels SMARCE1 HBV-infected HepG2.2.15 cells were measured by quantitative real-time PCR western blot analysis.HBV DNA replication was Southern analysis.The relative surface antigen e detected enzyme-linked immunosorbent assay.The Cell Counting Kit-8 (CCK-8) used to detect viability cells.The relationship between identified target prediction...
Abstract Background CircMAN2B2 is a newly discovered circRNA that has been found to be an oncogene in lung cancer and glioma. The present study was designed reveal the role of circMAN2B2 gastric carcinoma (GC). Methods qRT‐PCR method utilized examine expression GC tissues paracancerous tissues. Next, SNU‐16 AGS cells silenced by transfection. CCK‐8 assay, colony formation flow cytometer, Transwell Western blot were conducted for testing cell phenotype changes. Further, downstream genes...
Liver cancer is one of the most common human malignancies, and transforming growth factor-beta (TGF-β) pathway plays a key role in its pathogenesis. To study relationship between TGF-β related protein expression many signaling pathway, markers stem cells, CK family, others, liver HepG2 cells were transfected with siRNA directed against TGF-β1 or treated exogenous TGF-β1. Then, these levels measured by Western blotting. After transfection, level was decreased, indicating that it effective. In...
Summary Aims Ischemic preconditioning ( IPC ) has short‐term benefits for stroke patients. However, if protective effect is memorial and the role of intracellular protein heme oxygenase‐1 HO ‐1) not known. Methods corresponding sham control were achieved by blocking blood flow left internal carotid artery 20 min 2 second, respectively, in rats. Both sham‐operated animals divided into three groups treated with PBS , ‐1 inducer hemin, inhibitor Znpp. Three weeks after brain...
Pulmonary tuberculosis (TB) is a chronic respiratory infectious disease. Certain microRNAs (miRNAs or miRs) are reported to be involved in regulating TB progression. The present study aimed evaluate the diagnostic potential of miR‑125b pulmonary TB. expression levels and Raf1 proto‑oncogene serine/threonine protein kinase (RAF1) were analyzed via reverse transcription‑quantitative (RT‑q)PCR patients with correlation between clinical indicators was investigated, receiver operating...
OBJECTIVE To assess the difference of genetic alteration patterns among different areas in same oral squamous cell carcinoma (OSCC). METHODS Studied loss heterozygosity (LOH) and microsatellite instability (MI) at chromosomal loci TP53 RPS6 on invasive tumor front (ITF), center/superficial part stroma cells by combining laser capture microdissection (LCM) PCR technique. RESULTS There existed a high frequency LOH MI chromosomes RPS6. The aberration was 23.5% (4/17) 43.8% (7/16), respectively....
Objective To investigate the correlation between frequency of molecular abnormalities 4 loci at chromosomal 9p21 (D9S1747 ,D9S162, RPS6) and 17p13 (TP53) clinical characteristics prognosis. Methods The oral squamous cell carcinoma(OSCC) lesions in 71 patients were manually microdessected. Genomic DNA from these normal lymphnode tissu or peripheral blood same extracted using Watson's tissue kit. loss heterozygosity(LOH) microsatellite instability (MI) analyzed by PCR-page electrophoresis...
Purpose: The authors aimed to identify Notch signaling pathway gene mutations as a prognostic biomarker for bladder cancer. Methods: First, critical genes were screened using Cancer Genome Atlas and validation sets. Second, immune infiltration, protein-protein interaction network, Kyoto Encyclopedia of Genes Genomes Gene Set Enrichment Analysis analyses performed. Finally, potential immunotherapy drug targets T-cell receptors, B-cell receptors CERES scores Results: NOTCH7 was identified,...