A5004199883- Spine and Intervertebral Disc Pathology
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Sphingolipid Metabolism and Signaling
- Inflammasome and immune disorders
- RNA Interference and Gene Delivery
- Galectins and Cancer Biology
- interferon and immune responses
- Sarcoma Diagnosis and Treatment
- Osteoarthritis Treatment and Mechanisms
- Medical Imaging and Analysis
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Phagocytosis and Immune Regulation
- Musculoskeletal pain and rehabilitation
- Bone Tissue Engineering Materials
- Signaling Pathways in Disease
- Wnt/β-catenin signaling in development and cancer
- Extracellular vesicles in disease
- Plant Toxicity and Pharmacological Properties
- Epigenetics and DNA Methylation
- Cervical and Thoracic Myelopathy
Lanzhou University
2022-2025
Affiliated Hospital of Qingdao University
2024
Qingdao University
2012-2024
General Hospital of Guangzhou Military Command
2024
Chonnam National University
2024
Ludwig-Maximilians-Universität München
2023
LMU Klinikum
2023
Xuzhou Medical College
2009-2022
Second People’s Hospital of Huai’an
2009-2022
Huashan Hospital
2021-2022
A growing body of evidence has indicated that long non-coding RNAs (lncRNAs) serve as competing endogenous (ceRNAs) during oncogenesis. In this study, the qRT-PCR results lncRNA PVT1 is overexpressed in osteosarcoma and decreased survival rate patients. MTT clonal colony formation assays were used to detect effect on proliferation, flow cytometry was performed assess apoptosis cell cycle. Transwell assay analyze migration invasion. The revealed silencing by siRNA inhibited invasion promoted...
Osteosarcoma (OS) is the most common primary malignant bone tumor in adolescents and young adults. However, essential mechanisms underlying osteosarcomagenesis remain obscure. The HOTAIR, a well-known long noncoding RNA (lncRNA), involved pathogenesis progress of multiple tumors. To reveal potential role lncRNA HOTAIR OS carcinogenesis, we conducted two-stage, case-control study among Chinese population with 900 cases controls to evaluated associations its genetic variants risk. We found...
Bladder cancer is a severe with high mortality because of invasion and metastasis. Growing evidence has revealed that circular RNAs play critical roles in biological function, which closely connected to proliferation bladder cancer. In our study, we employed qRT-PCR, RNA fluorescence situ hybridization (FISH), 5-ethynyl-2′-deoxyuridine (EdU), CCK-8, Transwell assays, luciferase reporter xenografts, live imaging detect the binding protein multiple splicing (circRBPMS) (BC). Bioinformatics...
The pathogenesis of intervertebral disc degeneration (IDD) is still unclear. It has been shown that the pathological process IDD most closely related to inflammation nucleus pulposus cells (NPCs), in which inflammatory factors play an important role. Exosomes are main paracrine mediators and microvesicles with biological functions similar those from they derived. Studies have bone mesenchymal stem (BMSCs) can inhibit apoptosis NPCs by sending exosomes as anti-inflammatory antioxidant, proved...
The present study aimed to investigate the biological function and underlying molecular mechanisms of miR-31 in osteoarthritis (OA). Reverse transcription‑quantitative polymerase chain reaction was used detect miR‑31 expression, it found that downregulated cartilage tissues OA patients. microRNA.org predict gene targets miR‑31, dual luciferase reporter assays were verify C‑X‑C motif chemokine ligand 12 (CXCL12) a direct target miR‑31. human chondrocyte cell line CHON‑001 perform MTT...
Abstract Clear cell renal carcinoma (ccRCC) is one of the most common urogenital tumors with high mortality. Circular RNA (circRNA), as an emerging endogenous RNA, has been proved to play a crucial role in clear progression. In this study, we obtained circAFAP1 upregulated ccRCC by high-sequencing and verified qRT-PCR several cancer lines. situ hybridization (ISH) assays Kaplan–Meier plot showed higher level was linked shorter overall survival. Moreover, CCK8, colony formation, EdU...
Melatonin, a neuroendocrine hormone secreted by the pineal body, has positive effect on intervertebral disc degeneration. The present study is aimed at investigating biological role of melatonin in degeneration and its underlying mechanism. A human nucleus pulposus cell (NPC) line was exposed to different concentrations. Cell proliferation measured CCK-8 assay. cycle apoptosis were analyzed flow cytometry. Western blot performed measure protein expression indicated genes. rabbit model...
The treatment for intervertebral disc degeneration (IDD) has drawn great attention and recent studies have revealed that the p38 MAPK pathway is a potential therapeutic target delaying of discs.In this study, we analyzed nature-derived protein tyrosine kinase inhibitor, Genistein, its function in IDD rats both vitro vivo via pathway.Nucleus pulposus cells treated with Genistein showed better compared untreated cells.Further study could play protective role by inhibiting phosphorylation p38,...
Abstract Triple‐negative breast cancer (TNBC) is a malignant tumor with high degree of malignancy and lack effective target treatment. The research aims to explore the role mechanism X collagen alpha‐1 chain protein (COL10A1 gene) in TNBC. UALCAN Kaplan–Meier were used detect expression COL10A1 its prognosis patients. cells stably expressing levels obtained by recombinant lentivirus infection. was temporarily downregulated siRNA interference fragments. Real‐time quantitative polymerase...
The mammalian target of rapamycin (mTOR) has been reported to act as a gene microRNA (miR)-99a in various cancer cells and identified an independent prognostic marker human osteosarcoma. aim this study was investigate the clinical significance miR-99a/mTOR axis osteosarcoma.A total 130 pairs osteosarcoma matched noncancerous bone tissues were used detect expression levels miR-99a mTOR mRNA by quantitative real-time polymerase chain reaction. Then, associations and/or with...
Osteoarthritis (OA) is a common degenerative joint disease that may be closely linked to inflammation and oxidative stress destroying the balance of cartilage matrix. Theaflavin-3,3'-digallate (TFDG), natural substance derived from black tea, has been reported restrict activity inflammatory cytokines effectively eliminate reactive oxygen species (ROS) in various diseases. However, it not clear whether TFDG can improve OA. Chondrocytes were treated with or without IL-1β 20 μM 40 TFDG. The...
Osteosarcoma (OS) is a musculoskeletal malignancy that originates from interstitial cells. An increasing number of studies have verified long non‑coding RNAs (lncRNAs) participate in the progression numerous types cancer. It has been reported LINC00467 cancer‑promoting gene some cancer; however, regulatory mechanism OS remains unknown. In present study, reverse transcription-quantitative PCR was used to determine expression tissues and Additionally, impact LINC00467‑knockdown on cell...
The present study aimed at detecting the association of ovine major histocompatibility complex class II (Ovar II) DRB1 gene second exon and susceptibility or resistance to hydatidosis in three sheep breeds Sinkiang. MHC-DRB1 was amplified by polymerase chain reaction (PCR) from DNA samples healthy with hydatidosis. PCR products were characterized restriction fragment length polymorphism (RFLP) technique. Five enzymes, MvaI, HaeIII, SacI, SacII, Hin1I, used, yielding 14 alleles 31 patterns....
A previous study indicated that microRNA(miR)-328-3p upregulation might be critical for resveratrol-mediated suppression of metastatic ability in human osteosarcoma, implying its potential role this malignancy. However, the clinical significance and biological functions miR-328-3p osteosarcoma remain unclear.miR-328-3p expression 88 pairs matched non-cancerous bone tissues were detected by quantitative real-time PCR. Then, associations with various clinicopathological features patients...
The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for cancer therapeutics that exhibits the ability to preferentially induce apoptosis in malignant cells. RGD peptides bind integrins, ανβ3 and ανβ5, which are selectively expressed neovasculature at surface of certain To enhance antitumor effect, an RGD-TRAIL protein, TRAIL was fused with motif-containing cell adhesive sequence, GRGDNP (gly-arg-gly-asp-asn-pro), constructed evaluated bioactivity....