Sterol regulatory element binding protein 1 (SREBP‐1) is well‐known as the master regulator of lipogenesis in rodents. Acyl‐CoA synthetase short‐chain family member 2 (ACSS2) plays a key role by synthesizing acetyl‐CoA from acetate for lipogenesis. ATP citrate lyase (ACLY) catalyzes conversion and coenzyme A to acetyl‐CoA, hence, it also important Although ACSS2 function cancer cells has been elucidated, its essentiality ruminant mammary unknown. Furthermore, gene promoter mechanisms have...

Non-alcoholic fatty liver disease (NAFLD) has become a common in recent decades. No effective treatment is currently available. Probiotics and natural functional food may be promising therapeutic approaches to this disease. The present study aims investigate the efficiency of anthraquinone from Cassia obtusifolia L. (AC) together with cholesterol-lowering probiotics (P) improve high-fat diet (HFD)-induced NAFLD rat models elucidate underlying mechanism. Cholesterol-lowering were screened out...

Non-esterified fatty acids (NEFAs) promote de novo lipogenesis, which resulted in abnormal hepatic lipid accumulation, by NFκB-Orai1 pathway. Oxidative stress and endoplasmic reticulum (ER) have been recognized as key mechanisms non-alcoholic liver disease (NAFLD) pathogenesis. Whether Orai1 facilitates oxidative remains unknown. The rat model of NAFLD was constructed feeding high-fat diet (HFD). BRL-3A cells were treated NEFAs, Orai1inhibtor BTP2, NFκB inhibitor wogonin or small interfering...

Hypocalcemia in dairy cows is often associated with inflammation-related disorders such as metritis and mastitis. The protein encoded by the Ca2+ release-activated calcium modulator 1 (ORAI1) gene a membrane channel subunit that activated when stores are depleted. Polymorphonuclear neutrophils (PMNL) have crucial role defense against infection through migration, adhesion, chemotaxis, phagocytosis, reactive oxygen species (ROS) production response to pathogens. Whether hypocalcemia affects...

Fatty acid accumulation in hepatocytes induced by high concentrations of fatty acids due to lipolysis and the associated oxidative damage they cause occur most frequently after calving. Because their role esterification acids, diacylglycerol acyltransferase isoforms (DGAT1 DGAT2) could play a susceptibility dairy cows develop liver. To gain mechanistic insights, we performed vivo vitro analyses using liver biopsies or isolated primary hepatocytes. The study (n = 5 cows/group) involved...

Deep spoilage is a cyclical and costly problem for the meat industry. Mianning ham famous dry-cured product in Sichuan, China. The aim of this work was to investigate physicochemical characteristics, sources odor, associated microorganisms that cause ham. High-throughput sequencing solid-phase microextraction-gas-chromatography (SPME-GC-MS) techniques were used characterize properties, microbial community structure, volatile compounds spoiled compare it with normal results showed typically...

Triglyceride deposit cardiomyovasculopathy (TGCV) is an intractable cardiovascular disease for which a specific treatment urgently required. In TGCV, adipose triglyceride lipase (ATGL) deficiency results in the abnormal intracellular metabolism of long-chain fatty acid (LCFA) leads to TG deposition. Medium-chain triglycerides have been used as important functional food various human diseases. To address potential activities tricaprin, medium-chain triglyceride, on cardiac dysfunctions we...

The store-operated Ca2+ entry (SOCE) moiety ORAI calcium release-activated modulator 1 (ORAI1) located in the endoplasmic reticulum (ER) participates key cellular functions such as protein folding, transport, and secretion, lipid metabolism. We used an vitro approach to test whether exogenous fatty acids alter ORAI1 signaling explore potential consequences on mitochondrial dysfunction ER stress. First, hepatocytes isolated from 4 healthy female calves (1 d old, 40–50 kg) were challenged with...

The quality change and deterioration mechanism of leisure dried tofu (LD-tofu) were investigated during storage at 4 °C, 25 37 °C for 150 d. results indicated the protein content, fat carbohydrate pH LD-tofu significantly decreased, while total viable counts moisture content increased with a bigger time. Moreover, hardness springiness stored d 2.43 N 0.932, as well 1.44 0.842, respectively. Insights from low-field nuclear magnetic resonance measurements revealed that bound water in could be...

As a special breed in Asia, yellow-feather broilers have high nutrition and can deteriorate easily under chilled conditions. The present work evaluated the effect of different storage temperatures (−2, 1 4C) on shelf life chicken modified atmosphere packaging (CO2/N2: 80%/20%). total viable counts reached limit value (7.0 log colony-forming unit [cfu]/g) after 28 12 days at 4C, whereas it did not exceed 7.0 cfu/g during whole −2C period. samples stored had lowest volatile basic nitrogen...

In mammals, sterol regulatory element binding protein-1 (SREBP-1) is the master regulator of fatty acid and triacylglycerol synthesis. Recent gene silencing studies in mammary cells indicate that SREBP-1 has a central role milk fat However, knockdown goat have not been performed; hence, its direct controlling mRNA expression lipid metabolism genes synthesis remains unknown. Inhibition epithelial (GMEC) by small interference RNA (siRNA) markedly reduced content cellular (~50% decrease, P <...

Abstract Background Neddylation, an important post-translational modification (PTM) of proteins, plays a crucial role in follicular development. MLN4924 is small-molecule inhibitor the neddylation-activating enzyme (NAE) that regulates various biological processes. However, regulatory mechanisms neddylation rabbit ovarian cells have not been emphasized. Here, transcriptome and metabolome profiles granulosa (GCs) treated with were utilized to identify differentially expressed genes, followed...

Salt stress is an important factor restricting crop growth. Thus, genes related to plant responses high salinity at the sprout stage of common bean (Phaseolus vulgaris) should be identified and characterized. In this study, on basis germination index, 0.4% NaCl day 5 incubation period were selected as optimal salt concentration sampling time, respectively. Salt-tolerant salt-sensitive materials suitable for local region representative with contrasting salt-related phenotypes. A total 441...

Abstract To evaluate the effects of fatty acids on endoplasmic reticulum (ER) stress, oxidative and lipid damage. We treated BRL3A rat liver cells with, linoleic (LA), linolenic, oleic (OA), palmitic (PA), palmitoleic (POA), or stearic (SA) acid for 12 hr. The characteristics cell deposition, stress indexes, ER markers, nuclear factor κB p65 (NF‐κB p65), synthesis transport regulators, cholesterol metabolism regulators were analyzed. Endoplasmic chaperones like glucose‐regulated protein 78,...

Abstract Increased concentrations of free fatty acids (FFAs) induce reactive oxygen species (ROSs) generation and endoplasmic reticulum (ER) stress, thus, increasing the risk liver in dairy cows during periparturient period. In non-ruminants, Taraxasterol (Tara; a pentacyclic triterpenoid found medicinal plants) plays an important role anti-inflammatory antioxidant reactions. Whether Tara can alleviate or prevent ruminants is unknown. We addressed whether supply could dampen lipid...

Slc7a11 is the key component of system X c - , an antiporter that imports cystine (CySS) and exports glutamate. It plays important role in cellular defense against oxidative stress because cysteine (Cys), reduced from CySS, used for limits synthesis glutathione (GSH). We have shown downregulation responsible oxidation extracellular Cys/CySS redox potential lung fibroblasts old mice. However, how age-related change expression affects intracellular environment mouse remains unexplored. The...

Hypocalcemia in dairy cows is associated with decreased neutrophil phagocytosis, adhesion capacity, migration, and reactive oxygen species (ROS) production through alterations ORAI calcium release-activated modulator 1 (ORAI1). Neutrophils can resist the invasion of pathogenic microorganisms by releasing extracellular traps (NET). However, mechanisms controlling NET formation during hypocalcemia are unknown. To address role ORAI1 formation, neutrophils were isolated at 2 d postcalving from...

Abstract Fatty liver is a common metabolic disorder afflicting dairy cows during the periparturient period and closely associated with endoplasmic reticulum (ER) stress. The onset of ER stress in humans mice alters hepatic lipid metabolism, but it unknown if such event contributes to fatty soon after parturition. ORAI calcium release-activated modulator 1 (ORAI1) key component store-operated Ca2+ entry mechanism regulating cellular balance. purpose this study was investigate role ORAI1 on...

Common bean ( Phaseolus vulgaris ) is an important food crop; however, its production affected by salt stress. Salt stress can inhibit seed germination, promote senescence, and modify cell wall biosynthesis, assembly, architecture. Melatonin, indole heterocycle, has been demonstrated to greatly impact structure, composition, regulation in plants under However, the molecular basis for such assumptions still unclear. In this study, a common variety, “Naihua” was treated with water (W), 70...