A5039392004- Lipid metabolism and biosynthesis
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Rabbits: Nutrition, Reproduction, Health
- Gut microbiota and health
- Fatty Acid Research and Health
- Peroxisome Proliferator-Activated Receptors
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Research and Splicing
- Immune Response and Inflammation
- Animal Nutrition and Physiology
- Milk Quality and Mastitis in Dairy Cows
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- NF-κB Signaling Pathways
- Genetic and phenotypic traits in livestock
- Ubiquitin and proteasome pathways
- Virus-based gene therapy research
- Reproductive System and Pregnancy
- FOXO transcription factor regulation
Affiliated Hospital of Nantong University
2020-2025
Nantong University
2020-2025
Henan Agricultural University
2017-2024
Northwest A&F University
2012-2020
Soochow University
2015-2017
First Affiliated Hospital of University of South China
2017
University of South China
2017
Agriculture and Forestry University
2016
Southwest Minzu University
2016
MRC Laboratory of Molecular Biology
2015
Sterol regulatory element binding protein 1 (SREBP‐1) is well‐known as the master regulator of lipogenesis in rodents. Acyl‐CoA synthetase short‐chain family member 2 (ACSS2) plays a key role by synthesizing acetyl‐CoA from acetate for lipogenesis. ATP citrate lyase (ACLY) catalyzes conversion and coenzyme A to acetyl‐CoA, hence, it also important Although ACSS2 function cancer cells has been elucidated, its essentiality ruminant mammary unknown. Furthermore, gene promoter mechanisms have...
Coprophagy is of great significance to the growth, development, and reproductive performance rabbits. This study aimed at exploring effect coprophagy on New Zealand white rabbits by prevention (CP). The results showed that CP treatment significantly decreased growth development female live birth rate embryos. blood biochemical indexes increased contents serum ALB, ALP, MDA, while SOD activity was decreased. Transcriptome analysis GO terms were mainly enriched in transport function after...
Stearoyl-CoA desaturase 1 (SCD1) is a key enzyme for the synthesis of monounsaturated fatty acids (MUFA) palmitoleic acid and oleic acid. In non-ruminant species, SCD1 expression known to be tightly regulated by variety transcription factors. Although role transcriptional regulatory mechanism SREBP-1 PPARs in other species clear, changes lipid metabolism related via regulation or PPARG1 ruminant mammary tissue remain largely unknown. Here, we demonstrated that goat higher during lactation...
Abstract The scavenger receptor CD36 is involved in pathogen recognition, phagocytosis, and pathogen-induced signaling. This study investigated the relationship between TLR4 modifying lipopolysaccharide (LPS)-induced signaling pathways mediating Escherichia coli ( E. ) endocytosis primary goat mammary epithelial cells (pGMECs). manipulation of expression significantly influenced nuclear factor kappa B (NF-κB) mRNA pGMECs stimulated with LPS for 12 h. NF-κB activator protein-1 (AP-1) activity...
The microRNA-26 (miR-26) family is known to control adipogenesis in non-ruminants. genomic loci of miR-26a and miR-26b have been localized the introns genes encoding for proteins C-terminal domain RNA polymerase II polypeptide A small phosphatase (CTDSP) family. Insulin-induced gene 1 (INSIG1) encodes a protein with key role regulation lipogenesis rodent liver. In present study, we investigated synergistic function miR-26 their host goat mammary epithelial cells (GMEC). Downregulation...
Abstract Although research on dairy goat mammary gland have referred extensively to molecular mechanisms, lines of epithelial cells ( MECs ) are still rare. This paper sought establish an immortal MEC line by stable transfection human telomerase. from a lactating (45 days post‐parturition) X inong S aanen were cultured purely and subsequently transfected with plasmid carrying the sequence Immortalized telomerase (h T ‐ exhibited typical cobblestone morphology activity expression levels...
Abstract Sepsis‐induced myocardial dysfunction (SIMD), a deadly symptom in sepsis patients, is mainly caused by cardiovascular inflammation. However, it remains unclear how systemic inflammation triggers and aggravates the pathogenesis of SIMD. This study found that proinflammatory cytokines H 2 O concentrations were significantly induced SIMD‐mice. In particular, microarray analysis CD63 + exosomes isolated from sham‐ SIMD‐monocytes revealed significant induction thioredoxin‐interacting...
Abstract Background Coprophagy plays a vital role in maintaining growth and development many small herbivores. Here, we constructed coprophagy model by dividing rabbits into three groups, namely, control group (CON), sham-coprophagy prevention (SCP), (CP), to explore the effects of on performance cecal microecology rabbits. Results showed that CP treatment decreased feed utilization Serum total cholesterol triglyceride were remarkably lower than those other two groups. Furthermore, destroyed...
In mammals, sterol regulatory element binding protein-1 (SREBP-1) is the master regulator of fatty acid and triacylglycerol synthesis. Recent gene silencing studies in mammary cells indicate that SREBP-1 has a central role milk fat However, knockdown goat have not been performed; hence, its direct controlling mRNA expression lipid metabolism genes synthesis remains unknown. Inhibition epithelial (GMEC) by small interference RNA (siRNA) markedly reduced content cellular (~50% decrease, P <...
FoxO1 is a crucial transcription factor involved in lipid metabolism mouse liver through repressing key regulator of lipogenesis, sterol regulatory element binding protein 1 (SREBP1). However, it remains elusive whether plays roles the regulation fatty acid during lactation dairy goats. In this study, we aim to investigate function goat mammary epithelial cells (GMECs). We found that expression significantly upregulated compared with dry period. knockdown enhanced genes related de novo...
Abstract Background Neddylation, an important post-translational modification (PTM) of proteins, plays a crucial role in follicular development. MLN4924 is small-molecule inhibitor the neddylation-activating enzyme (NAE) that regulates various biological processes. However, regulatory mechanisms neddylation rabbit ovarian cells have not been emphasized. Here, transcriptome and metabolome profiles granulosa (GCs) treated with were utilized to identify differentially expressed genes, followed...
The main purpose of this study was to explore the effect tea tree oil (TTO) on lipopolysaccharide (LPS)-induced mastitis model using isolated bovine mammary epithelial cells (BMEC). This used determine cellular responses TTO and LPS cytotoxicity, mRNA abundance cytokine production. High-throughput sequencing select candidate genes, followed by functional evaluation those genes. In first experiment, at a concentration 200 μg/mL reduced cell proliferation, induced apoptosis upregulated protein...