Complete spinal cord gaps in adult rats were bridged with multiple intercostal nerve grafts that redirected specific pathways from white to gray matter. The grafted area was stabilized fibrin glue containing acidic fibroblast growth factor and by compressive wiring of posterior processes. Hind limb function improved progressively during the first 6 months, as assessed two scoring systems. corticospinal tract regenerated through lumbar enlargement, did several bulbospinal pathways. These data...

Abstract Human mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord were induced to transform into dopaminergic neurons in vitro through stepwise culturing neuron-conditioned medium, sonic hedgehog, and FGF8. The success rate was 12.7%, as characterized by positive staining for tyrosine hydroxylase (TH), rate-limiting catecholaminergic synthesizing enzyme, dopamine being released culture medium. Transplantation such striatum rats previously made Parkinsonian unilateral...

Human umbilical mesenchymal stem cells (HUMSCs) isolated from Wharton's jelly of the cord can be easily obtained and processed compared with embryonic or bone marrow cells. These may a valuable source in repair spinal injury.We examine effects HUMSC transplantation after complete transection rats. Approximately 5x10(5) HUMSCs were transplanted into lesion site. Three groups rats implanted either untreated (referred to as cell group), treated neuronal conditioned medium (NCM) for three days...

Several lines of evidence have demonstrated that bone marrow-derived mesenchymal stem cells (BM-MSC) release bioactive factors and provide neuroprotection for CNS injury. However, it remains elusive whether BM-MSC derived from healthy donors or stroke patients provides equal therapeutic potential. The present work aims to characterize prepared normal rats (NormBM-MSC) cerebral ischemia (IschBM-MSC), examine the effects their conditioned medium (Cm) on ischemic animal model. Isolated...

We successfully demonstrate the first solid-state sensor to have reliable responses breath ammonia of rat. For thioacetamide (TAA)-induced hepatopathy rats, we observe that proposed can detect liver undergoes acute–moderate with a p-value less than 0.05. The is an organic diode vertical nanojunctions produced by using low-cost colloidal lithography. Its simple structure and low production cost facilitates development point-of-care technology. also anticipate study starting point for...

Spinal cord injury elicits an inflammatory response that recruits macrophages to the injured spinal cord. Quantitative real-time PCR results have shown a repair strategy combining peripheral nerve grafts with acidic fibroblast growth factor (aFGF) induced higher interleukin-4 (IL-4), IL-10, and IL-13 levels in graft areas of rat cords compared transected at 10 14 d. This led arginase I-positive alternatively activated macrophage (M2 macrophage) responses. The gene expression several enzymes...

Object The study aimed to verify the safety and feasibility of applying acidic fibroblast growth factor (aFGF) with fibrin glue in combination surgical neurolysis for nonacute spinal cord injury. Methods This open-label, prospective, uncontrolled human clinical trial recruited 60 patients injuries (30 cervical 30 thoracolumbar). mean patient age was 36.5 ± 15.33 (mean SD) years, male/female ratio 3:1. time from injury treatment 25.7 26.58 months, cause included motor vehicle accident (26...

Abstract Glial cell line‐derived neurotrophic factor (GDNF) acts as a potent survival for many neuronal populations, including spinal motoneurons, indicating the therapeutic promise of GDNF neurological disorders. Injury to cord (SCI) triggers processes destructive ascending sensory and descending motor conduction extends tissue loss, thereby leading permanent behavioral dysfunction. In this study, we attempted examine whether protects neurons from SCI subsequently lessens locomotor deficit...

Acidic fibroblast growth factor (aFGF; also known as FGF-1) is a potent neurotrophic that affects neuronal survival in the injured spinal cord. However, pathological changes occur with cord injury (SCI) and attribution to aFGF of neuroprotective effect during SCI are still elusive. In this study, we demonstrated rat SCI, when treated aFGF, showed significant functional recovery indicated by Basso, Beattie, Bresnahan locomotor rating scale combined behavior score (p < 0.01-0.001). Furthermore...

Spinal cord injury (SCI) is a devastating neurological condition and might even result in death. However, current treatments are not sufficient to repair such damage. Bone marrow mesenchymal stem cells (BM-MSC) ideal transplantable which have been shown modulate the cascade of SCI mostly through paracrine effects. The present study investigates whether systemic administration conditioned medium from MSCs (MSCcm) has potential be efficacious as an alternative cell-based therapy for SCI. In...

Abstracts Background Spinal cord injury (SCI) is a complex and severe neurological condition. Mesenchymal stem cells (MSCs) their secreted factors show promising potential for regenerative medicine. Many studies have investigated MSC expansion efficacy of all kinds culture medium formulations, such as growth factor-supplemented or xeno-free medium. However, very few focused on the human (hMSC) formulations injured spinal repair. In this study, we effect hMSC-conditioned supplemented with...

In Brief Study Design. We present a case of patient with chronic paraplegia complete spinal cord gap resulting from stabbing injury 4 years ago recovering after an innovative surgical strategy. Objectives. To demonstrate the clinical outcome repair sural nerve graft fibrin glue containing acidic fibroblast growth factor in injury. Summary Background Data. Spinal usually causes permanent disability, and there had been not effective technique to obtain satisfactory functional motor recovery,...

The aim of this study was to assess functional outcomes nerve repair using acidic fibroblast growth factor (FGF) in patients with cervical spinal cord injury (SCI).Nine who had SCI for longer than 5 months were included pre- and postoperative assessments their neurological function. evaluating activities daily living, associated ability, degree spasticity, motor power, sensation, pain perception. After the first set assessments, authors repaired injured segment a total laminectomy followed...

Acute kidney injury is a major challenge in critical care medicine, with high rates of in-hospital morbidity and mortality. Stem cell therapy has emerged as an evolving technology that could have substantial impact on acute outcomes the environment. Therefore, authors investigated therapeutic effects adipose-derived stem cells ischemic rats.The used ischemia-reperfusion-induced rat model. The rescuing were assessed regard to different numbers various routes administration compared...

We tested the herbal extract 2,3,5,6-tetramethylpyrazine (TMP) for possible therapeutic efficacy against a glioma cell line and gliomas transplanted into rat brains. In cultured cells, 50 μM TMP significantly inhibited glutamate-induced increase in intracellular calcium. Significant damage (30%) proliferation suppression (10%), however, occurred only at higher concentrations (200-400 μM). Gliomaneuronal co-culturing resulted significant neuronal of cells (140%) compared with single cultures....

Abstract This study assesses the ability and potential of carbon nanotube (CNT)/chitosan to guide axon re‐growth after nerve injuries. The CNT/chitosan fibers were produced via coagulation hydrodynamic focusing method. Fiber width morphology adjusted using such parameters as syringe pumping rate coagulant used. fiber diameters 50–300 μm for rates 6–48 mL/h. Polyethylene glycol/NaOH (25%, w/w) solution was a suitable forming with small diameters. Physical property tests demonstrate that...