A5052853161- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Photochromic and Fluorescence Chemistry
- Atrial Fibrillation Management and Outcomes
- Plant Diversity and Evolution
- Cardiac electrophysiology and arrhythmias
- Sulfur Compounds in Biology
- Ferroptosis and cancer prognosis
- Innovation and Socioeconomic Development
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Nanowire Synthesis and Applications
- Neuroscience of respiration and sleep
- Venous Thromboembolism Diagnosis and Management
- Biotin and Related Studies
- Galectins and Cancer Biology
- Cancer, Lipids, and Metabolism
- Adenosine and Purinergic Signaling
- Cardiac tumors and thrombi
- Infective Endocarditis Diagnosis and Management
- RNA modifications and cancer
Yale University
2023-2025
First Affiliated Hospital of Xinjiang Medical University
2024-2025
Xinjiang Medical University
2024-2025
Peking University
2019-2024
Shanghai Pulmonary Hospital
2023-2024
State Key Laboratory of Natural and Biomimetic Drugs
2022-2024
Tongji University
2023-2024
Guizhou Normal University
2023
Second Military Medical University
2014-2020
SingHealth
2018
A chemical protein-DNA conjugation approach enhances precise genome editing in human cells and mouse zygotes.
Abstract The mechanism underlying unwanted structural variations induced by CRISPR-Cas9 remains poorly understood, and no effective strategy is available to inhibit the generation of these byproducts. Here we find that a high level translocations dependent on repeated cleavage at Cas9-targeting sites. Therefore, employ in which Cas9 fused with optimized TREX2 generate Cas9TX, exo-endonuclease, prevents perfect DNA repair thereby avoids cleavage. In comparison CRISPR-Cas9, CRISPR-Cas9TX...
Background: Recently, PANoptosis has aroused the interest of researchers for its role in cancers. However, studies that investigated lung cancer are still few. Methods: The public data were mainly collected from Cancer Genome Atlas (TCGA) and Gene Expression Omnibus database. R software was utilized analysis data. Quantitative real-time (qRT) polymerase chain reaction (PCR) used to measure RNA level FADD. cell proliferation ability evaluated using CCK8, colony formation,...
To investigate the mechanism of action Srg3 in acute lung injury caused by sepsis.First, a sepsis-induced rat model was established using cecal ligation and puncture (CLP). RNA sequencing (RNA-seq) used to screen for highly expressed genes (ALI), results showed that significantly upregulated. Then, SWI3-related gene 3 (Srg3) knocked down AAV9 vector vivo, changes ALI symptoms rats were analyzed. In vitro experiments conducted establishing cell lipopolysaccharide (LPS)-induced BEAS-2B cells...
Abstract Proteins capable of switching between distinct active states in response to biochemical cues are ideal for sensing and controlling biological processes. Activatable CRISPR‐Cas systems significant precise genetic manipulation sensitive molecular diagnostics, yet directly Cas protein function remains challenging. Herein, we explore anti‐CRISPR (Acr) proteins as modules create synthetic switches (CasPSs) based on computational chemistry‐directed rational interface engineering. Guided...
Abstract Atrial fibrillation (AF) and atrial flutter (AFL) are cardiac arrhythmias associated with high morbidity mortality. This cross-sectional observational study assesses the epidemiological burden of AF/AFL concerning metabolic risk factors using Global Burden Disease (GBD) 2021 database. Age-standardized disability-adjusted life years rate (ASDR), mortality, estimated annual percentage change were calculated. The Bayesian Age-Period-Cohort model was utilized to predict future trends in...
The pleiotropic effects of human disease and the complex nature gene-interaction networks require knock-in mice allowing for multiplexed gene perturbations. Here we describe a series with C57BL/6 background conditional or constitutive expression LbCas12a high-fidelity enhanced AsCas12a, which were inserted at Rosa26 locus. Cas12a in did not lead to discernible pathology enabled efficient genome engineering. We used retrovirus-based immune-cell engineering CD4+ CD8+ T cells, B cells...
Diabetes mellitus (DM) facilitates atrial fibrosis and increases the risk of fibrillation (AF). The underlying mechanism DM in causing AF remains mostly unknown potential therapeutic targets for DM‑induced are rarely reported. Hydrogen sulfide (H2S) has drawn considerable attention recent years its as a cardiovascular protector. Thus, aim present study was to investigate effect H2S on action. Sprague‑Dawley rats were divided into four groups, including control group, group DM+H2S group....
Abstract Proteins capable of switching between distinct active states in response to biochemical cues are ideal for sensing and controlling biological processes. Activatable CRISPR‐Cas systems significant precise genetic manipulation sensitive molecular diagnostics, yet directly Cas protein function remains challenging. Herein, we explore anti‐CRISPR (Acr) proteins as modules create synthetic switches (CasPSs) based on computational chemistry‐directed rational interface engineering. Guided...
Genetic code expansion (GCE) is a powerful technique for site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in living cells, which achieved through evolved aminoacyl-tRNA synthetase mutants. Stability important promoting enzyme evolution, and we found that many the mutants have reduced thermostabilities. In this study, characterized two novel pyrrolysyl-tRNA synthetases (PylRSs) derived from thermophilic archaea: Methanosarcina thermophila (Mt) flavescens (Mf)....
Abstract Currently CRISPR/Cas9 is a widely used efficient tool for gene editing. Precise control over the system with high temporal and spatial resolution essential studying regulation Here, we synthesized novel light‐controlled crRNA by coupling vitamin E photolabile linker at 5′ terminus to inactivate system. The modification did not affect ribonucleoprotein (RNP) formation of Cas9/crRNA/tracrRNA complexes but inhibit association RNP target DNA. Upon light irradiation, E‐caged was...
We recently developed a system to create human chimeric antigen receptor (CAR)-T cells using conjugated Cas12a (cCas12a) in which is covalently linked its CRISPR RNA (crRNA). This protocol describes site-specific modification of and the preparation Cas12a-crRNA complex bio-orthogonal chemistry, followed by CAR-T cell generation through electroporation AAV infection. shows robust editing efficiency can be used for precisely targeted, highly efficient integration CAR genes into T genome. For...
Abstract Cardiac myxomas are rare but manifested with risk of embolism and often cause unexpected symptoms or sudden death. We retrospectively collected the medical records patients diagnosed cardiac at center our university. Overall 465 were included in this study, group had significantly higher CHA 2 DS -VASc scores ( P = 0.005). In embolic group, stroke was recorded 110 (77.14%) patients, while events limbs observed 10 (2.15%) 9(1.93%) developed splenic infarction. Patients older age...
Abstract Objective Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are associated with significant mortality rates. The role of Fcgr2b in the pathogenesis ALI/ARDS is not fully elucidated. This study aimed to investigate functions explore its underlying mechanisms. Methods Methods: In this study, rat models ARDS pulmonary microvascular endothelial cell (PMVEC) were established through administration lipopolysaccharide (LPS). expression levels Elk1 quantified both...
Site-specific Cas9–crRNA conjugation reduces the synthetic burden of customized guide RNA.
Structurally diverse acylations have been identified as post-translational modifications (PTMs) on histone lysine residues, but their functions and regulations remain largely unknown. Interestingly, in nature, a acylation analog, pyrrolysine, is introduced co-translational modification (CTM) through genetic encoding. To explore this alternative life form, we created model organism Saccharomyces cerevisiae containing site-specific CTMs (acetyl-lysine, crotonyl-lysine, or another synthetic...
<sec><b>Objective</b> To evaluate the imaging potential of a novel near-infrared (NIR) probe conjugated to COC183B2 monoclonal antibodies (MAb) in ovarian cancer (OC). </sec><sec><b>Methods</b> The expression OC183B2 antigen OC was determined by immunohistochemical (IHC) staining using tissue microarrays with H-score system and immunofluorescence (IF) tumor cell lines. Imaging probes NIR fluorescent dye cyanine 7 (Cy7) Mab were chemically engineered. OC183B2-positive human cells (SKOV3-Luc)...
We assembled and characterized the complete chloroplast genome sequence of Chrysojasminum subhumile (W.W.Sm.) Banfi & Galasso 2014, a valuable horticultural medicinal plant species. The total size was 159,918 bp in length GC content 37.4%. It displayed circular structure could be divided into large single-copy region, small pair inverted repeat regions. encoded 131 unique genes, including 82 protein-coding 41 tRNA eight rRNA genes. Among these 17 contained single intron, two genes had...