A5100362902- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Cancer Cells and Metastasis
- Genomics and Phylogenetic Studies
- Prenatal Screening and Diagnostics
- Birth, Development, and Health
- Ferroptosis and cancer prognosis
- Viral Infections and Vectors
- Mycobacterium research and diagnosis
- Molecular Biology Techniques and Applications
- Cervical Cancer and HPV Research
- Hedgehog Signaling Pathway Studies
- Cancer-related Molecular Pathways
- Lymphoma Diagnosis and Treatment
- Vitamin D Research Studies
- Pharmaceutical studies and practices
- Impact of Technology on Adolescents
- Child Development and Digital Technology
- Cancer Mechanisms and Therapy
- Actinomycetales infections and treatment
- Human Health and Disease
- Health, Environment, Cognitive Aging
- Fetal and Pediatric Neurological Disorders
Hangzhou DAC Biotech (China)
2021-2024
Xi’an Children’s Hospital
2024
Soochow University
2018-2024
First Affiliated Hospital of Soochow University
2018-2024
Taihe Hospital
2024
Hubei University of Medicine
2024
Sun Yat-sen University
2024
Guangxi Medical University
2023
First Hospital of Shijiazhuang
2023
First Affiliated Hospital of Xi'an Jiaotong University
2023
All authors declared no competing interests for this work.
Aims Metagenomic next-generation sequencing (mNGS) has been utilized for diagnosing infectious diseases. It is a culture-free and hypothesis-free nucleic acid test all pathogens with known genomic sequences, including bacteria, fungi, viruses parasites. While this technique greatly expands the clinical capacity of pathogen detection, it second-line choice due to lengthy procedures microbial contaminations introduced from wet-lab processes. As result, we aimed reduce hands-on time exogenous...
Neonatal hypoxic-ischemic encephalopathy (HIE) is considered a major cause of death and long-term neurological injury in newborns. Studies have demonstrated that oxidative stress apoptosis play role the progression neonatal HIE. Echinocystic acid (EA), natural plant extract, shows great antioxidant antiapoptotic activities various diseases. However, it has not yet been reported whether EA exerts neuroprotective effect against Therefore, this study was undertaken to explore effects potential...
BackgroundHospitals in China are classified into tiers (1, 2 or 3), with the largest (tier 3) having more equipment and specialist staff. Differential health insurance cost-sharing by hospital tier (lower deductibles higher reimbursement rates lower tiers) was introduced to reduce overcrowding hospitals, promote use of limit escalating healthcare costs. However, little is known about effects differential schemes on choice tiers.MethodsIn a 9-year follow-up prospective study 0.5 M adults from...
Abstract Introduction Metagenomic Next‐Generation Sequencing (mNGS) is an emerging technique for microbial identification and diagnosis of infectious diseases. The clinical utility mNGS, especially its real‐world impact on antimicrobial treatment patient outcome has not been systematically evaluated. Methods We prospectively assessed the effectiveness mNGS in 70 febrile inpatients with suspected infections at Hematology department Children's Hospital, National Clinical Research Center Child...
Microbial infections cause significant morbidity and mortality in neonates. Metagenomic next-generation sequencing is a hypothesis-free culture-free test that enables broad identification of pathogens antimicrobial resistance genes directly from clinical samples within 24 h. In this study, we used mNGS for etiological diagnosis monitoring the efficacy antibiotic treatment cohort neonatal patients with severe infections. The median age was 19.5 (3-52) days, gestational 37.96 (31-40+3) weeks,...
Objective Chromosomal abnormalities such as aneuploidy have been shown to be responsible for causing spontaneous abortion. Genetic evaluation of abortions is currently underperformed. Screening in the products conception can help determine etiology. We designed a high-throughput ligation-dependent probe amplification (HLPA) assay examine 24 chromosomes miscarriage tissues and aimed validate performance this technique. Methods carried out screening 98 fetal tissue samples collected from...
The relationship between cyclin D1 (CCND1) rs9344 G>A polymorphism and colorectal cancer (CRC) risk is still ambiguous. To obtain a precise estimation of the relationship, we performed an extensive meta-analysis based on eligible studies. Crude odds ratios with their 95% confidence intervals were harnessed to determine strength correlation CCND1 CRC under allele, homozygote, dominant, recessive genetic models, respectively (28 studies 5,784 cases 7,858 controls). Our results indicated...
We diagnosed a clinical case of pulmonary infection involving Mycobacterium tuberculosis and Tropheryma whipplei in patient with acute respiratory distress syndrome. The diagnosis was assisted by metagenomic next-generation sequencing bronchoalveolar lavage fluid.A 44-year-old Han Chinese inmate transferred to the emergency department because dry cough, chest tightness, shortness breath. patient's body temperature rose 39.3 °C following empirical cephalosporin treatment for 1 week. blood...
Accurate and rapid identification of causative pathogens is essential to guide the clinical management lower respiratory tract infections (LRTIs). Here we conducted a single-centre prospective study in 284 patients suspected evaluate utility nucleic acid test based on highly multiplexed polymerase chain reaction (PCR) CRISPR-Cas12a.
The traditional treatment for small-cell lung cancer (SCLC) has been systemic platinum-containing chemotherapy because the response rate is 50–90%. Durvalumab an immune checkpoint inhibitor that blocks binding of programmed cell death protein 1 and ligand 1. combined with agents recommended as first-line extensive-stage SCLC, but its use may cause immune-related adverse events. Autoimmune encephalitis a rare potentially fatal neurological event. This current case report describes male...
Abstract BACKGROUND Noninvasive prenatal screening (NIPS) using plasma cell-free DNA has gained tremendous popularity in the clinical assessment of fetal aneuploidy. Most, if not all, these tests rely on complex and expensive massively parallel sequencing (MPS) techniques, hindering use NIPS as a common procedure. METHODS We have developed optimized an MPS-independent noninvasive genetic test that can rapidly detect aneuploidy at considerably lower costs. used high-throughput...
Pythium insidiosum is a rare fungus-like pathogen that known to cause pythiosis in mammals with high morbidity and mortality. Identification of the essential for timely treatment rational use antibiotics. However, difficult detect via conventional microbiological tests. The current gold standard polymerase chain reaction, which lacking most hospitals since human China. In this study, we used metagenomic Next-Generation Sequencing identified 56-year-old Chinese male who was hospitalized due...
Introduction: Non-Hodgkin lymphoma (NHL) has a much higher incidence rate than Hodgkin lymphoma. Approximately 40% NHL occurs in extranodal tissues or organs, and its clinical manifestations are often nonspecific. Primary bone involving the mandible is an uncommon that characterized by fever, gum swelling toothache. Therefore, it misdiagnosed as oral diseases. Case Presentation: A 52-year-old female had recurrent fever for more 1 month, with numbness her left jaw PET/CT showed uptake area...
Low birth efficiency and developmental abnormalities in embryos derived using round spermatid injection (ROSI) limit the clinical application of this method. Further, underlying molecular mechanisms remain elusive warrant further in-depth study. In study, embryonic day (E) 11.5 mouse fetuses corresponding placentas upon ROSI, intracytoplasmic sperm (ICSI), natural vivo fertilized (control) were collected. Transcriptome DNA methylation profiles analyzed compared RNA-sequencing (RNA-seq)...
Vitamin D receptor (VDR) gene polymorphisms have been reported to increase susceptibility some malignant tumors, yet the effect on gastric cardiac adenocarcinoma remains unknown. Here, we conducted a hospital-based case-control study examine correlation of single nucleotide VDR rs2107301T>C, rs2228570C>T, rs1989969C>T and rs11568820 G>A susceptibility. A total 330 cases 608 controls were enrolled in study. Using ligation detection reaction, found that variant alleles four not associated with...
High-risk human papillomaviruses (HPVs) play a cardinal role in the etiology of cervical cancer. The most prevalent type, HPV-16, shows intratypic sequence variants that are known to differ oncogenic potential and geographic distribution. Intratype variations E6/E7 capsid L1 proteins HPV-16 associated with risk viral persistence progression.This study was designed analyze E6, E7, genes patients lesion identify novel northern China.Our results showed respect E6 E7 were high prevalence Asian...
BackgroundVenous thromboembolism (VTE) has both environmental and genetic risk factors. It is regulated by polygenes multisites. The polygenic score (PRS) been widely used because any single biomarker failed to accurately predict the of VTE. However, no model proposed for VTE in Chinese population. Thus, we aimed construct a PRS first episode population.MethodsFirst, nucleotide polymorphisms (SNPs) associated with genome-wide association studies, meta-analyses, candidate gene studies were...