Sirtuin 2 (Sirt2) is known to negatively regulate anoxia-reoxygenation injury in myoblasts. Because protein levels of Sirt2 are increased ischemia-reperfusion (I/R)-injured liver tissues, we examined whether protective or detrimental against hepatic I/R injury. We overexpressed the C57BL/6 mice using a adenovirus. Wild-type and knockout were subjected partial (70%) ischemia for 45 minutes, followed by various periods reperfusion. In another set experiments, wild-type pretreated...

The prevalence of metabolic diseases has risen globally in parallel with the obesity epidemic over past few decades. Green tea been reported to have metabolically beneficial effects on obesity; however, mechanism by which green regulates lipid metabolism is not clearly understood. Male c57BL/6 mice were fed a normal chow diet, high-fat diet (HFD), or an HFD supplemented various doses epigallocatechin gallate-rich extract (GTE) for 12 weeks. GTE supplementation reduced body weight gain,...

Abstract Protein kinase 2 (CK2) activation was reported to enhance reactive oxygen species production and activate the nuclear factor κB (NF-κB) pathway. Because oxidative stress inflammation are critical events for tissue destruction during ischemia reperfusion (I/R), we sought determine whether CK2 important in renal response I/R. Mice underwent 25 min of were then reperfused. We confirmed an increased expression CK2α period, while CK2β remained consistent. administered...

Allium cepa L. (onion) has been reported to have various pharmacological effects, such as preventing heart disease, and improving antimicrobial activity immunological effects. The Republic of Korea produced 1,195,563 tons onions (2022). flesh onion is used food while the skin (OS) thrown away an agro-food by-product considered induce environmental pollution. Thus, we hypothesize that increasing usage OS functional material could help protect from environment antioxidant effects...

Augmenting glucose utilization in skeletal muscle via the phosphatidylinositol-3 kinase (PI3 kinase)/protein B (Akt) pathway or adenosine monophosphate (AMP)-activated protein (AMPK) is necessary to regulate hyperglycemia patients with type 2 diabetes mellitus.We investigated effect of mulberry leaf extract (MLE) on uptake cells and explored its vivo antidiabetic potential.Male db/db mice were treated either MLE (50 mg/kg, 100 250 mg/kg) metformin (100 for 8 weeks.MLE treatment stimulated...

In this study, we focus on the effects of n-3 polyunsaturated fatty acids (PUFAs) tunicamycin-, streptozotocin-, or high fat diet (HFD)-induced β-cell damage and dysfunction.Pretreatment with PUFAs protected RINm5F cells mouse islets against tunicamycin-induced through suppression ER stress apoptosis induction. This protective effect β-cells was further demonstrated by normalization insulin secretion in response to glucose tunicamycin-treated islets. multiple low-dose streptozotocin-induced...

Opuntia ficus-indica (OF) phytochemicals have received considerable attention because of their health benefits. However, the structure-activity relationship between saponin and flavonoid antioxidant compounds among secondary metabolites has rarely been reported. In a molecular docking study, selected from both callus (OFC) OF ethanol extract were found to be involved in Toll-like receptor 4 mitogen-activated protein kinase (MAPK) signaling pathways. High affinity was specific for MAPK, it...

Previously, powdered persimmon leaves have been reported to glucose‑ and lipid‑lowering effects in diabetic (db/db) mice. As leaf is commonly consumed as tea, an aqueous extract of (PLE) was prepared its anti‑diabetic efficacy investigated. In the present study, PLE tested for inhibitory activity on α‑glucosidase vitro. An oral maltose tolerance test performed Next, acute effect examined streptozotocin‑induced Last, long‑term supplementation assessed db/db after eight weeks. glucose test,...

Hepatic ischemia/reperfusion (I/R) injury leads to oxidative stress and acute inflammatory responses that cause liver damage have a considerable impact on the postoperative outcome. Much research has been performed develop possible protective techniques. We aimed investigate efficacy of SPA0355, synthetic thiourea analog, in an animal model hepatic I/R injury. Male C57BL/6 mice underwent normothermic partial ischemia for 45 min followed by varying periods reperfusion. The animals were...

Nonalcoholic steatohepatitis (NASH) arises from nonalcoholic fatty liver disease (NAFLD) as a consequence of oxidative stress. Gynostemma pentaphyllum extract (GPE) is proven to be beneficial for patients suffering NAFLD. However, the precise mechanism by which GPE confers these benefits remains largely unknown. The purpose this study was investigate underlying and determine whether supplementation with newly discovered gypenoside UL4 mitigates NASH progression. Male c57BL/6 mice were fed...

The prevention and management of type 2 diabetes mellitus has become a major global public health challenge. Decursin, an active compound Angelica gigas Nakai roots, was recently reported to have glucose-lowering activity. However, the antidiabetic effect extract (AGNE) not yet been investigated. We evaluated effects AGNE on glucose homeostasis in diabetic mice investigated underlying mechanism by which acts. Male C57BL/KsJ-db/db were treated with either (10 mg/kg, 20 40 mg/kg) or metformin...

The generation of nitric oxide (NO) via inducible NO synthase (iNOS) and reactive oxygen species plays a key role in cytokine-mediated pancreatic β-cell damage. Oxidative stress due to activates the nuclear factor-κB (NF-κB) transcription factor, which regulates iNOS expression. In this regard, suppression NF-κB pathway is novel strategy for protecting β-cells from This study was performed explore effects kazinol U, prenylated flavan Broussonetia kazinoki, on activation interleukin-1β...

Oenanthe javanica, commonly known as water dropwort, has long been used to treat acute and chronic hepatitis, abdominal pain, alcohol hangovers, inflammation in various traditional medicine systems Asia. However, whether O. javanica beneficial effects on colitis-induced intestinal damage remains elusive. This study tested the hypothesis that anti-inflammatory antioxidant activities mice with dextran sulfate sodium (DSS)-induced colitis. First, treatment of ethanol extract (OJE) inhibited...

The axis of nuclear factor κB (NF-κB)-inducible NO synthase (iNOS)-nitric oxide plays a key role in cytokine- and streptozotocin-mediated pancreatic β-cell damage. In this study, we investigated the effects kazinol C isokazinol D isolated from Broussonetia kazinoki on viability function. RINm5F cells primary islets were used for vitro ex vivo cytokine toxicity experiments, respectively. For type 1 diabetes induction, mice injected with multiple low-dose streptozotocin (MLDS)....

Allium hookeri is widely consumed as a vegetable and herbal medicine in Asia. A. has been reported anti-inflammatory, anti-obesity, osteoblastic, anti-oxidant, anti-diabetic effects animal studies. We investigated the of aqueous extract (AHE) Korean subjects. Prediabetic subjects (100 ≤ fasting plasma glucose (FPG) < 126 mg/dL) who met inclusion criteria were recruited for this study. The enrolled (n = 30) randomly divided into either an AHE 15, 486 mg/day) or placebo 15) group. Outcomes...

We investigated the immune-stimulating and anti-diabetic effects of Allium hookeri leaves grown in a plant factory with artificial lights. The immunomodulatory A. leaves’ ethanol extracts were evaluated immune-related hematological factors blood, proliferation splenocytes, NK cell activity, IgG cytokine levels, their mechanisms immunosuppressed obese mice. Anti-diabetic determined by inhibitory activity against α-amylase α-glucosidase vitro fasting blood glucose levels biochemical serum leaf...

Cytokines activate several inflammatory signals that mediate β-cell destruction. We recently determined SPA0355 is a strong anti-inflammatory compound, thus reporting its efficacy in protecting β cells from various insults. The effects of on survival were studied RINm5F and primary islets. protective this compound the development type 1 diabetes evaluated non-obese diabetic (NOD) mice. completely prevented cytokine-induced nitric oxide synthase (iNOS) expression cytotoxicity isolated...

Type 1 diabetes is an autoimmune disease caused by permanent destruction of insulin-producing pancreatic β cells and requires lifelong exogenous insulin therapy. Recently, islet transplantation has been developed, although there have significant advances, this approach not widely used clinically due to the poor survival rate engrafted islets. We hypothesized that improving islets through ex vivo genetic engineering could be a novel strategy for successful transplantation. transduced with...

Abstract Insulin-like growth factor-1 (IGF-1) is known to inhibit reperfusion-induced apoptosis. IGF-binding protein-3 (IGFBP-3) the major circulating carrier protein for IGF-1 and induces In this study, we determined if IGFBP-3 was important in hepatic response I/R. To deliver IGFBP-3, used an adenovirus containing cDNA (AdIGFBP-3) or mutant devoid of IGF binding affinity but retaining receptor ability (AdIGFBP-3 GGG ). Mice subjected I/R injury showed typical patterns hepatocellular...

Although multiple studies have shown that Angelica keiskei of the Umbelliferae family has potent anti-inflammatory and antioxidative activities it reduces serum bile acids in humans, whether A. protective effects against cholestasis-induced liver injury remains unexplored until now. This study tests hypothesis root extract (AKE) alleviates injury, inflammation, fibrosis mouse models acute cholestasis induced by duct ligation (BDL). Oral administration AKE (200 or 500 mg/kg) attenuated...

Jieun Jung, Jeong Wook Heo, Ji Su Kim, Un Yul Jeong, Ui-Jin Bae, Ha Na Jang, Chang Ki Shim, Yoonjo Joung, and Sung Hyen Lee. Journal of the Korean Society Food Science Nutrition 2022;51:1355-63. https://doi.org/10.3746/jkfn.2022.51.12.1355