A5008108221- Pleural and Pulmonary Diseases
- Occupational and environmental lung diseases
- Lung Cancer Diagnosis and Treatment
- Ultrasound in Clinical Applications
- RNA modifications and cancer
- NF-κB Signaling Pathways
- Lung Cancer Treatments and Mutations
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Amoebic Infections and Treatments
- Pneumothorax, Barotrauma, Emphysema
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Medical Imaging and Pathology Studies
- Bone and Dental Protein Studies
- COVID-19 diagnosis using AI
- CAR-T cell therapy research
- Infective Endocarditis Diagnosis and Management
- Lung Cancer Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Trauma Management and Diagnosis
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
Oxford University Hospitals NHS Trust
2016-2025
University of Oxford
2016-2024
Churchill Hospital
2016-2024
National Institute for Health Research
2018-2024
Science Oxford
2022-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2023
Oxford BioMedica (United Kingdom)
2020-2023
Oxford Biomedical Research
2021-2023
Translational Research in Oncology
2022-2023
University of Patras
2014-2023
Abstract Oncolytic viruses exploit the cancer cell phenotype to complete their lytic life cycle, releasing progeny virus infect nearby cells and repeat process. We modified oncolytic group B adenovirus EnAdenotucirev (EnAd) express a bispecific single‐chain antibody, secreted from infected tumour into microenvironment. This T‐cell engager (Bi TE ) binds Ep CAM on target cross‐links them CD 3 T cells, leading clustering activation of both 4 8 cells. Bi transcription can be controlled by major...
Mast cells (MCs) have been identified in various tumors; however, the role of these tumorigenesis remains controversial. Here, we quantified MCs human and murine malignant pleural effusions (MPEs) evaluated fate function MPE development. Evaluation MPE-competent lung colon adenocarcinomas revealed that tumors actively attract subsequently degranulate space by elaborating CCL2 osteopontin. were required for effusion development, as MPEs did not form mice lacking MCs, infusion with...
Abstract Malignant pleural effusion (MPE) is the lethal consequence of various human cancers metastatic to cavity. However, mechanisms responsible for development MPE are still obscure. Here we show that mutant KRAS important induction in mice. Pleural disseminated, bearing tumour cells upregulate and systemically release chemokine ligand 2 (CCL2) into bloodstream mobilize myeloid from host bone marrow space via spleen. These promote formation, as indicated by splenectomy splenocyte...
Rationale: Assessing the early use of video-assisted thoracoscopic surgery (VATS) or intrapleural enzyme therapy (IET) in pleural infection requires a phase III randomized controlled trial (RCT). Objectives: To establish feasibility randomization versus non-surgery as well key outcome measures which are important to identify relevant patient-centered outcomes RCT. Methods: MIST-3 was prospective multicenter All-comers with confirmed diagnosis were enrolled and those ongoing sepsis after up...
Lung cancer and chronic lung diseases impose major disease burdens worldwide are caused by inhaled noxious agents including tobacco smoke. The cellular origins of environmental-induced tumors the dysfunctional airway alveolar epithelial turnover observed with unknown. To address this, we combined mouse models genetic labeling ablation (club) cells exposure to environmental carcinogenic agents. Club shown survive KRAS mutations form after carcinogen exposure. Increasing numbers club found in...
Background Accurate survival estimation in malignant pleural effusion is essential to guide clinical management strategies and inform patient discussion. The LENT PROMISE scores were developed aid prognostication effusion, however their uptake practice has been limited. We aimed conduct a detailed external validation of the develop recommendations regarding utility, highlight factors limiting performance. Methods Medical records patients diagnosed with between 2015–2023 at Oxford University...
Although oncogenic activation of NFκB has been identified in various tumors, the NFκB-activating kinases (inhibitor kinases, IKK) responsible for this are elusive. In study, we determined role IKKα and IKKβ KRAS-mutant lung adenocarcinomas induced by carcinogen urethane respiratory epithelial expression KRASG12D Using reporter mice conditional deletions IKKβ, two distinct early late phases during chemical genetic adenocarcinoma development, which were characterized nuclear translocation...
Rationale: Sonographic septations are assumed to be important clinical predictors of outcome in pleural infection, but the evidence for this is sparse. The inflammatory and fibrinolysis-associated intrapleural pathway(s) leading septation formation have not been studied a large cohort fluid (PF) samples with confirmed infection matched ultrasound data. Objectives: To assess presence severity against baseline PF PAI-1 (Plasminogen-Activator Inhibitor-1) other proteins as well correlate these...
The lungs are ubiquitous receptacles of metastases originating from various bodily tumors. Although osteopontin (SPP1) has been associated with tumor dissemination, the role its isoforms in lung-directed metastasis is incompletely understood. We employed syngeneic mouse models spontaneous and induced lung-targeted C57BL/6 mice competent deficient both Spp1 alleles. Tumor-derived expression was modulated using either stable anti-Spp1 RNA interference, or forced overexpression intracellular...
Research Article24 March 2017Open Access Source DataTransparent process NRAS destines tumor cells to the lungs Anastasios D Giannou Laboratory for Molecular Respiratory Carcinogenesis, Department of Physiology, Faculty Medicine, University Patras, Rio, Greece Search more papers by this author Antonia Marazioti Nikolaos I Kanellakis orcid.org/0000-0002-0065-2282 Ioanna Giopanou Ioannis Lilis Dimitra E Zazara Giannoula Ntaliarda Danai Kati Vasileios Armenis Georgia A Giotopoulou Anthi C...
Abstract Cancer remains one of the leading causes mortality worldwide, to increased interest in utilizing immunotherapy strategies for better cancer treatments. In past decade, CD103 + T cells have been associated with clinical prognosis patients cancer. However, specific immune mechanisms contributing toward CD103-mediated protective immunity remain unclear. Here, we show an unexpected and transient CD61 expression, which is paired at synaptic microclusters cells. colocalization cell...
Malignant pleural effusion (MPE) is a frequent metastatic manifestation of human cancers. While we previously identified KRAS mutations as molecular culprits MPE formation, the underlying mechanism remained unknown. Here, determine that non-canonical IKKα-RelB pathway activation KRAS-mutant tumor cells mediates development and this fueled by host-provided interleukin IL-1β. Indeed, IKKα required for MPE-competence activating NF-κB signaling. IL-1β fuels addiction mutant to resulting in...
Patients with malignant pleural mesothelioma (MPM) or metastases often present effusion (MPE). This study aimed to analyze the effect of fluid on cancer cells.Established patient-derived cell cultures derived from MPE (MPM, breast carcinoma, lung adenocarcinoma) were seeded in 100% (exudate MPM MPE, transudate non-MPE fluid) and proliferation was monitored. In addition, establishment new cultures, specimens, attempted by seeding cells fluid.All established proliferated similar growth rates...
Increased expression of osteopontin (secreted phosphoprotein 1, SPP1) is associated with aggressive human lung adenocarcinoma (LADC), but its function remains unknown. Our aim was to determine the role SPP1 in smoking-induced LADC. We combined mouse models tobacco carcinogen-induced LADC, deficiency endogenous Spp1 alleles, and adoptive pulmonary macrophage reconstitution map receptors impact during carcinogenesis. Co-expression mutant KrasG12C benign cells employed investigate SPP1/KRAS...
Lung adenocarcinoma (LADC) is the leading cause of cancer death worldwide. Nevertheless, syngeneic mouse models disease are sparse, and cell lines suitable for transplantable immunocompetent LADC remain unmet needs. We established multiple by repeatedly exposing two strains (FVB, Balb/c) to tobacco carcinogens urethane or diethylnitrosamine culturing out resulting lung tumours prolonged periods time. Characterization (n = 7) showed that they were immortal phenotypically stable in vitro,...
ABSTRACT Background and objective Chemical pleurodesis is performed for patients with MPE a published success rate of around 80%. It has been postulated that inflammation key in achieving successful pleural symphysis, as evidenced by higher amounts pain or detected inflammatory response. Patients mesothelioma are to have lower due lack normal tissue enabling an Methods The TIME1 trial data set, which were co‐primary outcome measures, was used address number these assumptions. Pain score,...