A5085969573- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Sodium Intake and Health
- Adenosine and Purinergic Signaling
- Diabetes Management and Research
- Adipose Tissue and Metabolism
- Phosphodiesterase function and regulation
- Peripheral Artery Disease Management
- Blood Pressure and Hypertension Studies
- Nitric Oxide and Endothelin Effects
- Diabetes Treatment and Management
- Apelin-related biomedical research
- Mobile Health and mHealth Applications
- Cardiovascular Health and Disease Prevention
- Heart Failure Treatment and Management
- Vascular Procedures and Complications
- Magnesium in Health and Disease
- Cardiovascular Disease and Adiposity
- Cancer, Stress, Anesthesia, and Immune Response
- Eicosanoids and Hypertension Pharmacology
- Systemic Lupus Erythematosus Research
- Cardiovascular Syncope and Autonomic Disorders
- Lipoproteins and Cardiovascular Health
- Electrolyte and hormonal disorders
Saiseikai Ibaraki Hospital
2015-2025
Saiseikai Yokohama Eastern Hospital
2019-2021
Yokohama City University
1994-2012
Yokohama City University Hospital
2010-2012
Hamamatsu Medical Center
2006
Mitsui Memorial Hospital
1997-2000
Institute of Applied Biochemistry
1994
University of Tsukuba
1994
Background A positive association was previously reported between angiotensin-converting enzyme (ACE) gene polymorphism and several cardiovascular diseases, such as myocardial infarction, left ventricular hypertrophy, restenosis after percutaneous transluminal coronary angioplasty. Plasma ACE activity carotid-wall thickening measured by ultrasonography were related, it postulated that long-term exposure to high levels of plasma could be involved in structural changes the arterial wall. In...
Various neurotransmitters, such as dopamine, stimulate adenylyl cyclase to produce cAMP, which regulates neuronal functions. Genetic disruption of the type 5 isoform led a major loss activity in striatum-specific manner with small increase expression few other isoforms. D1 dopaminergic agonist-stimulated was attenuated, and this accompanied by decrease receptor Gsα. D2 agonist-mediated inhibition also blunted. Type cyclase-null mice exhibited Parkinsonian-like motor dysfunction, i.e....
Abstract Background This is the phase 3 study in Japan designed to verify superiority of patiromer over placebo using change serum potassium level (sK-level). Methods was a multicenter, randomized withdrawal targeting Japanese hyperkalemic patients. It consisted run-in period and double-blind period. The an active single-arm, open-label (4 or 5 weeks). randomized, placebo-controlled, parallel-group, Patients whose sK-level within normal range at week 4 entered who were randomly assigned...
Abstract Background The Y-AIDA study was designed to investigate the renal- and home blood pressure (BP)-modulating effects of add-on dapagliflozin treatment in Japanese individuals with type 2 diabetes mellitus (T2DM) albuminuria. Methods We conducted a prospective, multicenter, single-arm study. Eighty-six patients T2DM, HbA1c 7.0–10.0%, estimated glomerular filtration rate (eGFR) ≥ 45 mL/min/1.73 m , urine albumin-to-creatinine ratio (UACR) 30 mg/g creatinine (gCr) were enrolled, 85 these...
Abstract We evaluated the effectiveness of a mobile health (mHealth) intervention for diabetic kidney disease patients by conducting 12-month randomized controlled trial among 126 type 2 diabetes mellitus with moderately increased albuminuria (urinary albumin-to-creatinine ratio (UACR): 30-299 mg/g creatinine) recruited from eight clinical sites in Japan. Using Theory Planned Behavior (TPB) behavior change theory framework, provides detailed information order to improve patient control over...
Abstract Adenosine plays several roles in the kidney mediated by specific receptors A 1 , 2 and possibly 3 . We studied localization of adenosine receptor mRNA rat nephron segments using reverse transcription polymerase chain reaction (RT-PCR). The male Sprague-Dawley rats (6 to 8 weeks old) were microdissected. Total RNA was prepared acid-guanidinium–phenol–chloroform method used following RT-PCR assay. Because PCR primers spanned no intron, samples reacted absence RT as controls for...
Renin is produced mainly by the kidney, and cAMP a main positive regulator of its synthesis. This study was undertaken to analyze molecular mechanism cAMP-mediated regulation Ren-1C gene transcription proximal promoter. We first showed that promoter region from -365 +16 mouse renin (Ren-1C) mediated cAMP-induced chloramphenicol acetyltransferase expression in embryonic kidney-derived 293 cells. Deletion analysis heterologous assay disclosed -75 able activate cAMP, indicated element -47 (RP-2...
Background Diabetic kidney disease (DKD) is one of the main complications type 2 diabetes mellitus (T2DM). DKD a known risk factor for end-stage renal disease, cardiovascular and all-cause death. Effective intervention early-stage vital to slowing down progression improve prognoses. Mobile health (mHealth) reportedly effective in supporting patients’ self-care improving glycemic control, but impact mHealth on has yet be shown. Objective The purpose this study evaluate efficacy standard...
Abstract To investigate the relation between angiotensin-converting enzyme (ACE) gene polymorphism and acute coronary syndromes with respect to environmental factors, we analyzed association of genotype angiographic findings patients myocardial infarction or unstable angina pectoris, examined linkage each established risk factors for artery disease. We determined ACE in 152 Japanese 399 healthy individuals. The distributions were not different two groups ( P =.74, χ 2 test). In former group,...
Abstract Background A novel hemodialysis (HD) system employing electrolyzed water containing molecular hydrogen (E-HD) has been developed to improve the bio-compatibility of HD. This study examined impact E-HD on changes in redox state during HD and HD-related fatigue. Method single-arm, prospective observational 63 patients chronic (41 males; mean age, 72 ± 9 years; median duration HD, 7 years). Redox parameters (serum myeloperoxidase [MPO], malondialdehyde-protein adduct [MDA-a],...
Angiotensinogen gene expression is controlled in a tissue- and development-specific manner. Interestingly, the angiotensinogen abundantly expressed adipose tissues other than liver, where it mainly produced. We investigated molecular mechanism of 3T3-L1 preadipocyte-adipocyte system. Although mRNA was barely detectable preadipocytes, its levels increased significantly during differentiation. As whole, pattern change transcriptional activity promoter similar to that adipogenic...
The two stimulatory G protein alpha subunits, Galphas and Galphaolf, activate adenylyl cyclase in a similar way. We examined whether type 5 knockout, the major striatal isoform, can differentially and/or developmentally change expression of these proteins striatum. Galphaolf expressions at birth were unaffected knockouts, which, however, demonstrated blunted developmental increase but not Galphas. Adenylyl activity was birth, subsequently became lower knockouts. These findings suggest that...
1. We investigated the effects of direct blockade angiotensin II (AngII) by a potent, non-peptide toff 1 (AT1) receptor antagonist, TCV 116, on development cardiac hypertrophy in salt-loaded Dahl salt-sensitive rats. 2. Six week old male rats (n = 44) were fed 4% salt diet and simultaneously given various doses 116 orally for 7 weeks. Each control group received vehicle alone. 3. Dietary high intake elevated blood pressure continuously caused left ventricular treated with vehicle. had minor...
We investigated the impact of basal dietary sodium intake on dapagliflozin-induced changes in albuminuria and blood pressure (BP) measured at home patients with diabetic kidney disease (DKD).This was a secondary analysis Y-AIDA Study, which DKD estimated glomerular filtration rate (eGFR) ≥ 45 ml/min/1.73 m2 urinary albumin-to-creatinine ratio (UACR) 30 mg/g creatinine were administered dapagliflozin for 24 weeks, significantly improved levels BP profiles. The effects UACR, home-measured BP,...
Recently, restriction fragment length polymorphism (RFLP) of alpha 2-adrenergic receptor gene (alpha 2-C10) digested with Bsu36I enzyme has been reported in US populations. Therefore, we examined the association this RFLP essential hypertension by comparing frequency specific alleles for Japanese The distribution was compared that Subjects were hypertensive patients a family history (n = 56) and normotensive subjects whose parents had no 46). DNA prepared from leukocytes. determined use...
Objectives: Sarcopenia and peripheral artery disease (PAD) are both risks of cardiovascular all-cause mortality. We aimed to investigate whether PAD or decline in ankle-brachial index (ABI) is associated with sarcopenia muscle loss patients life-style related diseases. Methods: studied 689 being treated measured ABI, as an lower limb blood flow, skeletal mass (SMI), by using method bioelectrical impedance analysis. was defined ABI % 0.90 diagnosed the criteria Asian Working Group for...