Yangchan Li
A5065323962- RNA modifications and cancer
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Acute Lymphoblastic Leukemia research
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- RNA Research and Splicing
- Medical Imaging Techniques and Applications
- Radiation Dose and Imaging
- Advanced Radiotherapy Techniques
- CRISPR and Genetic Engineering
- Childhood Cancer Survivors' Quality of Life
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- HVDC Systems and Fault Protection
City of Hope
2019-2025
Beckman Research Institute
2020-2025
The First Affiliated Hospital, Sun Yat-sen University
2020-2024
Sun Yat-sen University
2020-2024
City Of Hope National Medical Center
2023
Abstract Background While liver cancer stem cells (CSCs) play a crucial role in hepatocellular carcinoma (HCC) initiation, progression, recurrence, and treatment resistance, the mechanism underlying CSC self-renewal remains elusive. We aim to characterize of Methyltransferase 16 (METTL16), recently identified RNA N 6 -methyladenosine (m A) methyltransferase, HCC development/maintenance, stemness, as well normal hepatogenesis. Methods Liver-specific Mettl16 conditional KO (cKO) mice were...
Rationale: Despite substantial advancement in the treatment of B-cell acute lymphoblastic leukemia (B-ALL), it remains a leading cause cancer mortality children due to high relapse rate. Moreover, long-term survival rates for adult B-ALL patients are still less than 40%. The carrying MLL rearrangements or BCR-ABL fusion represent high-risk subtypes that face particularly dismal prognoses. This study aims identify innovative therapeutic vulnerability B-ALL. Methods: CRISPR-Cas9 screen was...
Although the overall survival rate of B cell acute lymphoblastic leukemia (B-ALL) in childhood is more than 80%, it merely 30% refractory/relapsed and adult patients with B-ALL. This demonstrates a need for improved therapy targeting this subgroup Here, we show that ten-eleven translocation 1 (TET1) protein, dioxygenase involved DNA demethylation, overexpressed plays crucial oncogenic role independent its catalytic activity Consistent B-ALL, overexpression TET1 alone normal precursor cells...
Colorectal cancer (CRC) remains a significant cause of related mortality. Fat mass and obesity-associated protein (FTO) is m6A mRNA demethylase that plays an oncogenic role in various malignancies. In this study we evaluated the FTO CRC tumorigenesis.Cell proliferation assays were conducted 6 cell lines with inhibitor CS1 (50-3200 nM) (± 5-FU 5-80 mM) after lentivirus mediated knockdown. Cell cycle apoptosis HCT116 cells (24 h 48 h, 290 nM CS1). Western blot dot plot performed to assess...
Abstract Background: As the most prevalent internal decorations in mammalian mRNA, N6-methyladenosine (m6A) has been reported to be involved many physiological and pathological processes, including acute myeloid leukemia (AML). METTL3 METTL14, well-recognized m6A methyltransferase complex, contribute AML. METTL16 is a recently identified that deposit few targets. While, unlike METTL3/14, biological functions of are largely unknown. Here, we explored function mechanism AML pathogenesis...
Retrospective studies indicate that radiation damage to left anterior descending coronary artery (LAD) may be critical for late-stage radiation-induced cardiac morbidity. Developing a method accurately depicts LAD motion and perform dose assessment is crucial.
A combination of immunotherapy and chemotherapy strategies could strengthen antitumor effects. This protocol elucidates a robust method via co-culturing drug pre-treated acute myeloid leukemia cells with CD3+ T cells, derived from leukoreduction system chambers, for in vitro vivo study. We optimized several aspects the procedures, including timing treatment, quantification tumor approach treatment vivo. enables readouts interplay between drugs cells. For complete details on use execution...
Abstract Metastatic triple negative breast cancer (TNBC) patients have a 5-year survival rate of just 12%. Therefore, there is an urgent need to discover novel molecular mechanisms that contribute TNBC metastasis such as N6-methyladenosine (m6A) RNA modification. To do this, we conducted selective CRISPR-cas9 screen 14 m6A-regulators and identified m6A reader protein, YTHDC1, essential gene for the MDA-MB-231 cells. The Cancer Genome Atlas (TCGA) dataset showed with poorer prognosis had...
Abstract Introduction: Albeit that over 170 types of chemical modifications have been identified in RNAs, the biological functions most those decorations are still elusive. N7-methylguanosine (m7G), routinely occurring at 5’ cap mRNA or within tRNA and rRNA, also exists internal mRNA. However, “reader” proteins recognize m7G regulate metabolism fate target mRNAs yet to be identified. Here, we aim identify reader(s) elucidate function. Methods: To reader(s), utilized RNA-pull down, mass...