A5028945083- Bioinformatics and Genomic Networks
- Genetic Mapping and Diversity in Plants and Animals
- Gene expression and cancer classification
- Biomedical Text Mining and Ontologies
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Receptor Mechanisms and Signaling
- Genomics and Rare Diseases
- CRISPR and Genetic Engineering
- Health, Environment, Cognitive Aging
- Epigenetics and DNA Methylation
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Dermatology and Skin Diseases
- Genetic Associations and Epidemiology
- Neurotransmitter Receptor Influence on Behavior
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- Microbial Natural Products and Biosynthesis
- vaccines and immunoinformatics approaches
- Medical and Biological Ozone Research
Medical College of Wisconsin
2017-2025
Marquette University
2018-2019
Versiti Blood Center of Wisconsin
2010
Jagiellonian University
2005-2007
Holy Cross University
2002
Abstract The Alliance of Genome Resources (Alliance) is a consortium the major model organism databases and Gene Ontology that guided by vision facilitating exploration related genes in human well-studied organisms providing highly integrated comprehensive platform enables researchers to leverage extensive body genetic genomic studies these organisms. Initiated 2016, building central portal (www.alliancegenome.org) for access data primary along with gene ontology data. All types represented...
Abstract The Rat Genome Database (RGD, https://rgd.mcw.edu) has evolved from simply a resource for rat genetic markers, maps, and genes, by adding multiple genomic data types extensive disease phenotype annotations developing tools to effectively mine, analyze, visualize the available data, empower investigators in their hypothesis-driven research. Leveraging its robust flexible infrastructure, RGD added human eight other model organisms (mouse, 13-lined ground squirrel, chinchilla, naked...
Formed in late 1999, the Rat Genome Database (RGD, https://rgd.mcw.edu) will be 20 2020, Year of Rat. Because laboratory rat, Rattus norvegicus, has been used as a model for complex human diseases such cardiovascular disease, diabetes, cancer, neurological disorders and arthritis, among others, >150 years, RGD always disease-focused committed to providing data tools researchers doing comparative genomics translational studies. At its inception, before sequencing rat genome, started with only...
The seventh iteration of the reference genome assembly for Rattus norvegicus-mRatBN7.2-corrects numerous misplaced segments and reduces base-level errors by approximately 9-fold increases contiguity 290-fold compared with its predecessor. Gene annotations are now more complete, improving mapping precision genomic, transcriptomic, proteomics datasets. We jointly analyzed 163 short-read whole-genome sequencing datasets representing 120 laboratory rat strains substrains using mRatBN7.2. defined...
Rat models have been a major model for studying complex disease mechanisms, behavioral phenotypes, environmental factors, and drug development discovery. Inbred rat strains control genetic background allow repeated, reproducible, cellular whole animal phenotyping. The Hybrid Diversity Panel (HRDP) was designed to be powerful panel of inbred rats with genomic, physiological, data serve as resource systems genetics. HRDP consists 96-98 aimed maximize power detect specific loci associated...
Abstract The Rat Genome Database (RGD) is a multispecies knowledgebase which integrates genetic, multiomic, phenotypic, and disease data across 10 mammalian species. To support cross-species, multiomics studies to enhance expand on manually extracted from the biomedical literature by RGD team of expert curators, imports multiple sources. These include major databases substantial number domain-specific resources, as well direct submissions individual researchers. incorporation these diverse...
Summary Since it was previously shown that protein antigens applied epicutaneously in mice induce allergic dermatitis mediated by production of T helper 2 (Th2) cytokines we postulated this might suppression Th1 immunity. Here show epicutaneous immunization normal with a different antigen on the skin form patch induces state subsequent antigen‐non‐specific unresponsiveness caused suppressor cells (Ts) inhibit sensitization and elicitation effector T‐cell responses. Suppression is...
Abstract Biological interpretation of a large amount gene or protein data is complex. Ontology analysis tools are imperative in finding functional similarities through overrepresentation enrichment terms associated with the input lists. However, most limited by their ability to do ontology-specific and species-limited analyses. Furthermore, some not updated frequently recent information from databases, thus giving users inaccurate, outdated uninformative data. Here, we present MOET...
Protein antigens applied epicutaneously by the patch method induce allergic dermatitis mediated IgE antibodies in mice and simultaneously significant suppression of Th1-mediated delayed-type hypersensitivity (DTH) reactions.We developed a which protein (calf collagen, elastin, keratin, TNP-substituted mouse Ig) were homogenized with neutral cream. Animals treated such preparations tested for contact sensitivity to TNP hapten or DTH hemocyanin. Antigen specificity induced unresponsiveness was...
The electrochemical oxidation of α-, γ- and δ-tocopherols, as well α-tocopheryl acetate were investigated by voltammetry at platinum microelectrodes in glacial acetic acid. Particular attention has been devoted to the mechanism electrode process α-tocopherol solutions containing sodium perchlorate background electrolyte. involves an initial loss one electron proton form appropriate radical. exhibits adsorption phenomena on Pt surface. optimal conditions for voltammetric determination have...
The seventh iteration of the reference genome assembly for
Abstract Rare diseases individually affect relatively few people, but as a group they impact considerable numbers of people. The Rat Genome Database (https://rgd.mcw.edu) is knowledgebase that offers resources for rare disease research. This includes definitions, genes, quantitative trail loci (QTLs), genetic variants, annotations to published literature, links external resources, and more. One important resource identifying relevant cell lines rat strains serve models Diseases, have report...
Substance use disorder (SUD) is associated with a cluster of cognitive disturbances that engender vulnerability to ongoing drug seeking and relapse. Two these endophenotypes-risky decision-making impulsivity-are amplified in individuals SUD are augmented by repeated exposure illicit drugs. Identifying genetic factors underlying variability behavioral patterns critical for early identification, prevention, treatment SUD-vulnerable individuals. Here, we compared risky different facets...
Rats have been used as research models in biomedical for over 150 years. These disease arise from naturally occurring mutations, selective breeding and, more recently, genome manipulation. Through the innovation of genome-editing technologies, genome-modified rats provide precision by disrupting or complementing targeted genes. To facilitate use these data produced rat models, Rat Genome Database (RGD) organizes strains and annotates with qualitative phenotype terms well quantitative...
Many non-coding SNPs identified in Genome-Wide Association Studies (GWAS) likely affect BP-related gene expression through epigenetic mechanisms. This study analyzes the comparative genomic and epigenomic landscapes human rat kidney tissues, including proximal tubule (PT) medullary thick ascending limb (mTAL), focusing on major challenge of identifying conserved regulatory elements intergenic regions. The purpose our is to identify BP regions that are from for vivo validation mechanistic...
GWAS has identified >1,000 blood pressure (BP)-associated sentinel SNPs, most of which (>90%) are intronic and intergenic. How they regulate BP-relevant genes remains an open question. We hypothesized many SNPs would reside within cis -regulatory elements specific to nephron segments critical sodium resorption such as proximal tubule (PT) thick ascending limb (TAL). To address this, we developed chromatin state (ATAC-seq) transcriptome (RNA-seq) maps from manually dissected human PT...
BACKGROUND: Hypertension or elevated blood pressure (BP) is a worldwide clinical challenge and the leading primary risk factor for kidney dysfunctions, heart failure, cerebrovascular disease. The central regulator of BP by maintaining sodium-water balance. Multiple genome-wide association studies revealed that heritable quantitative trait, modulated several genetic, epigenetic, environmental factors. SNPs identified in predominantly (>95%) reside within noncoding genomic regions, making...
ABSTRACT The acronym PHACE stands for the co-occurrence of posterior brain fossa malformations, hemangiomas, arterial anomalies, cardiac defects, and eye abnormalities. majority patients have a segmental hemangioma at least one developmental structural anomaly. etiology pathogenesis are unknown. Here we discuss candidate causative genes identified in de novo analysis whole genome sequencing germline samples from 98 unrelated trios which probands had PHACE, all sequenced as part Gabriella...