A5065725257- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Genital Health and Disease
- Cervical Cancer and HPV Research
- Urinary Tract Infections Management
- Microbial infections and disease research
- Gut microbiota and health
- Adolescent Sexual and Reproductive Health
- Microbial Community Ecology and Physiology
- HIV, TB, and STIs Epidemiology
- Alcohol Consumption and Health Effects
- Liver Disease Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Blood groups and transfusion
- Urological Disorders and Treatments
- Fungal Infections and Studies
- Pelvic floor disorders treatments
- HIV/AIDS Research and Interventions
- Sex work and related issues
- HIV, Drug Use, Sexual Risk
- Bacterial Identification and Susceptibility Testing
- Autoimmune Bullous Skin Diseases
- Reproductive Physiology in Livestock
- Bacteriophages and microbial interactions
- Nail Diseases and Treatments
State Research Center for Dermatovenereology and Cosmetology
2020-2024
Central Research Institute of Epidemiology
2008-2020
Russian Research Center for Molecular Diagnostics and Therapy
2017
Peoples' Friendship University of Russia
2012
Whole genome sequence analysis (digital DNA-DNA hybridization and average nucleotide identity) was carried out for 81 sequenced full genomes of the genus Gardnerella, including ten determined in this study, indicated existence 13 genomic species, which five consist only one strain contain more than four genomes. Furthermore, a collection Gardnerella strains, representing emended species G. vaginalis newly described leopoldii, piotii swidsinskii, studied. Matrix-assisted laser desorption...
Background: Colonic microbiome is thought to be involved in auto-immune multiple sclerosis (MS). Interactions between diet and the colonic MS are unknown. Methods: We compared composition of microbiota quantitatively 25 patients 14 healthy controls.Fluorescence situ hybridization (FISH) with 162 ribosomal RNA derived bacterial FISH probes was used. Ten received a ketogenic for 6 months. Changes concentrations 35 numerically substantial groups were monitored at baseline 2, 12, 23/24 weeks....
Azithromycin has been widely used for Mycoplasma genitalium treatment internationally. However, the eradication efficacy substantially declined recent decade. In Russia, josamycin (another macrolide) is recommended first-line M. infections, however, no data regarding with and resistance in infections have internationally published. We examined prevalence males attending an STI clinic Moscow, Russia from December 2006 to January 2008, investigated male urethritis, monitored DNA dynamics...
Bacterial vaginosis is characterized by an imbalance of the vaginal microbiome and a characteristic biofilm formed on epithelium, which initiated dominated Gardnerella bacteria, frequently refractory to antibiotic treatment. We investigated endolysins type 1,4-beta-N-acetylmuramidase encoded prophages as alternative When recombinantly expressed, these proteins demonstrated strong bactericidal activity against four different species. By domain shuffling, we generated several engineered with...
In this study, we performed an evaluation of the new CE ‐marked multiplex real‐time AmpliSens N.gonorrhoeae/C.trachomatis/M.genitalium/T.vaginalis‐ MULTIPRIME ‐ FRT PCR assay compared to APTIMA tests, i.e., COMBO 2 assay, Trichomonas vaginalis ( FDA ‐approved), and two different Mycoplasma genitalium assays (research use only; one them only used for discrepancy analysis). Vaginal swabs (n = 209) first‐void urine FVU ) specimens from females 498) males 554), consecutive attendees 1261) at a...
Bacterial vaginosis (BV) is a vaginal disorder characterized by depletion of the normal lactobacillus-dominant microbiota and overgrowth mainly anaerobic bacteria. The study aimed to evaluate distribution abundance Gardnerella vaginalis clades sialidase A gene in samples from Russian women, investigate if G. count detects an abnormal characteristic BV more accurately than load. Vaginal 299 non-pregnant patients gynecological clinics were examined using Nugent scores clade quantitative...
Background and objective Resistance in the sexually transmitted bacterium Mycoplasma genitalium to all recommended therapeutic antimicrobials have rapidly emerged. However, date, internationally reported resistance surveillance data for M. strains circulating Eastern Europe are entirely lacking. The aim of this study was estimate prevalence macrolide fluoroquinolone resistance-associated mutations four cities Russia one Estonia, 2013–2016. Materials methods Consecutive urogenital samples...
The aim of the study was to evaluate diagnostic value Nugent score, wet mount microscopy, and polymerase chain reaction (PCR) test developed in Russia for bacterial vaginosis (BV) diagnosis.One hundred Caucasian women were enrolled this study. Three vaginal samples taken from each participant: 1 PCR analysis, score evaluation, microscopy. smears microscopy air-dried sent Femicare, Tienen, Belgium, blinded analysis by Multiplex real-time performed using primers probes targeting Gardnerella...
Introduction Clue cells (epithelial heavily covered with adherent bacteria) are an accepted clue to the diagnosis of bacterial vaginosis. However, exact morphologic criteria and adherence were never elaborated. Materials Methods We investigated adhesive cohesive patterns main microbiota groups in vaginal discharge using fluorescence situ hybridization (FISH). Samples from 500 women diagnosed vaginosis positive for classic microscopy collected 42 gynecologic practices Berlin reexamined our...
The frequency ADH2-2 allele in the Moscow urban population and a correlation between allele, alcoholic dependence without cirrhosis, symptomatic cirrhosis status on hepatitis B C infection have been studied. One hundred twenty-three inhabitants of (50 healthy donors, 36 patients with (subdivided into infected uninfected by HBV and/or HCV) 37 dependence) similar age/sex drinking pattern analysed. 41% for is characteristic an population. This value intermediate that found Asian peoples Central...
Macrolide (MLR) and fluoroquinolone (FQR) resistance in Mycoplasma genitalium (MG) has recently become a major problem worldwide. The available data on the prevalence of MLR FQR MG Russia are limited. In this study, we aimed to evaluate pattern mutations 213 MG-positive urogenital swabs from patients Moscow between March 2021 2022. MLR- FQR-associated were searched 23S rRNA as well parC gyrA genes using Sanger sequencing. was 55/213 (26%), with A2059G A2058G substitutions being two most...
Traditional microscopy-based methods for diagnosis of bacterial vaginosis (BV) are underutilized in many settings, and molecular techniques may provide opportunities rapid, objective, accurate BV diagnosis. This study evaluated the quantitative AmpliSens Florocenosis/Bacterial vaginosis-FRT multiplex real-time PCR (Florocenosis-BV) assay. Vaginal samples from a previous including unselected female subjects (n = 163) using Amsel criteria 454 pyrosequencing were examined with Florocenosis-BV...
To estimate the prevalence and factors associated with HIV five other STIs among outdoor female sex workers (OSFW) indoor FSW (IFSW). Methods Cross-sectional survey using respondent-driven sampling methodology. Participants answered a bio-behavioural questionnaire were tested for Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Trichomonas vaginalis (TV), syphilis (lifetime contact) Mycoplasma genitalium (MG). Weighted 95% CIs calculated. multivariate logistic regression was performed...
This study aimed to assess the laboratory diagnosis of Neisseria gonorrhoeae in St. Petersburg, Russia. In total, 334 consecutive symptomatic patients were enrolled. Cervical and urethral specimens from women (n=286) men (n=48) analyzed by microscopy, culture two in‐house NAATs, i.e. polymerase chain reaction (PCR) nucleic acid sequence‐based amplification (NASBA), developed All N. gonorrhoeae‐ positive samples confirmed using porA pseudogene 16S rRNA gene sequencing. methods displayed 100%...
Background Several lymphogranuloma venereum (LGV) outbreaks among men who have sex with (MSM) been reported throughout the world since 2003. Nevertheless, no LGV cases internationally from Russia. We evaluated prevalence of MSM attending proctologists in Moscow, Russia, and compared non-LGV rectal Chlamydia trachomatis (CT) infections. Methods ( n = 534) for proctologic care were included. Rectal specimens sampled CT Neisseria gonorrhoeae (NG) by nucleic acid amplification tests (NAATs). All...
Testing of antibiotic resistance intact vaginal microbiota in pure culture is not feasible.Metronidazole, antiseptic octenisept®, antimycotic ciclopirox, bacterial probiotic Lactobacillus crispatus, yeast Saccharomyces boulardii, Gardnerella-phage-endolysin named phagolysin and combination with probiotics were tested for bacteriolytic activity. Included swabs from 38 random women Amsel-confirmed vaginosis (BV). Test aliquots incubated by 37° 2 24 h. Gardnerella, low G+C, Atopobium,...
Men who have sex with men (MSM) a high risk of lifelong anal cancer caused by high-risk human papillomavirus (HR HPV) infections. The aim this study was to investigate the prevalence canal HR HPV infection among and without HIV in Moscow (Russia). We evaluated associations some coinfections (HSV CMV) distribution MSM infection.Two groups HIV-positive (n = 60) HIV-negative were study. Fourteen (HR) types, HSV1/2, CMV investigated swabs.HR HPVs found nearly same frequency 66.7% both groups:...