Bacterial vaginosis (BV) is a vaginal disorder characterized by depletion of the normal lactobacillus-dominant microbiota and overgrowth mainly anaerobic bacteria. The study aimed to evaluate distribution abundance Gardnerella vaginalis clades sialidase A gene in samples from Russian women, investigate if G. count detects an abnormal characteristic BV more accurately than load. Vaginal 299 non-pregnant patients gynecological clinics were examined using Nugent scores clade quantitative...

Abstract The large majority of studies investigating associations between bacterial vaginosis (BV) and sexually transmitted infections (STIs) have been conducted among predominantly young women with high risk for STIs. Since a risky sexual behavior is significant factor both STIs BV, this creates bias toward an increased association BV This study evaluated BV-associated vaginal microbiota ( Chlamydia trachomatis , Mycoplasma genitalium Trichomonas vaginalis Neisseria gonorrhoeae ) in...

Background: Vaginal microbiota is a factor that determines woman’s health. Infectious complications of pregnant women, women in labor and newborns are often associated with significant change its composition. Analysis the species diversity vaginal during pregnancy postpartum period primarily contributes to study physiological processes concept “healthy” environment these periods life. Aim: The aim this was evaluate dynamic composition microorganisms before delivery at different times natural...

Background: The role of the microbial factor in genesis early miscarriages (up to 10 weeks pregnancy) has not been established. However, disruption vaginal microbiota may be associated with occurrence late miscarriage. Aim: aim this study was evaluate frequency and determine prognostic significance pregnancy registration. Materials methods: This involved 113 pregnant women registered before gestation, who were divided into two groups. Group I consisted 22 whose ended miscarriage (subgroup IA...

Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due high costs limited availability of NGS, multiplex real-time PCR draws more attention. The present study aimed evaluate microbial composition dominant lactobacilli species non-pregnant women with bacterial using RT-PCR test its diagnostic significance. In...

Abstract Background In Russia, nationally developed nucleic acid amplification tests (NAATs), which have never been validated to international commercially available NAATs, are mainly used in the diagnosis of Chlamydia trachomatis infection. Objective To evaluate performance characteristics six NAATs widely diagnose C. infection Russia. Materials and methods total, 446 consecutive symptomatic patients (319 females 127 males) were included. Five polymerase chain reaction (PCR) assays one...

Introduction. Colonization of the reproductive organs pregnant women with group B streptococci (GBS; Streptococcus agalactiae) can lead to severe perinatal and neonatal pathology. In modern conditions, aside from prevention antenatal infection fetus during childbirth using antibacterial drugs, vaccination is also necessary. this regard, surveillance GBS genotypes an important task. Objective. To determine molecular genetic determinants virulence agalactiae isolated newborns, monitor...

Objectives Antimicrobial resistance in Mycoplasma genitalium (MG) is a poorly surveyed and controlled global health concern. We evaluated the first commercial dual assay, AmpliSens M. -ML/FQ-Resist-FL for detection of potential macrolide quinolone resistance-associated mutations (MRAMs QRAMs, respectively) estimated prevalence these MG St. Petersburg, Russia. Methods Urogenital samples positive (n=145 from 2007 to 2020) negative (n=56 2021) routine diagnostics were retrospectively analysed...

This review summarizes literature data on the structure, biological characteristics, and virulence factors of Gardnerella vaginalis. Genotypic phenotypic diversity bacterium, as well its role in bacterial vaginosis are highlighted.

The study investigates colonization of the genitourinary tract and rectum in pregnant women by Streptococcus agalactiae early pregnancy, as well pregnancy outcomes for mother infant depending on use antibiotic prophylaxis. frequency GBS was 15.9 %, with detection rate urine being 8.6 vagina - 3.5 10 %. efficiency amoxicillin/clavulanic acid administered II trimester 66.7 Pregnancy receiving those not prophylaxis were studied. In all term labor occurred, healthy full-term infants born. one...

Aim. To evaluate the effectiveness of Alloferon (Allokin-alfa) in complex treatment virus-associated chronic endometritis (CE) patients with infertility, papillomavirus infection (PVI) persisting uterine cavity, and recurrent herpes-virus localized genital area.
 Materials methods. A prospective (n=33) open randomized (2:1) study was conducted to assess efficacy CE PVI, infection, aged 25 37 years (median age 31 [29; 32.5] years). All received valacyclovir therapy at 500 mg once daily...

Objective. To assess the importance of identifying different genotypes Gardnerella vaginalis in diagnosis recurrent bacterial vaginosis. Materials and methods. The study involved 299 women reproductive age. All patients were divided into three groups (healthy women, with first episode vaginosis, vaginosis). DNA vaginal discharge was detected by real-time PCR. detection four G. performed using multiplex quantify amplified PCR fragments, quantitative standard samples constructed. Statistical...

<h3>Background</h3> Young people are worldwide a risk group for sexually transmitted infections (STIs) and primary target screening. Knowledge on STI prevalence in the youths is essential to elaborate preventive measures. Self-sampling has been shown be an effective approach screening epidemiological programmes. This study aimed assess of <i>Chlamydia trachomatis</i>,<i> Neisseria gonorrhoeae</i>,<i> Mycoplasma genitalium </i>and <i>Trichomonas vaginalis</i> young St. Petersburg, Russia...

BACKGROUND: A microbial imbalance in the vaginal biotope leads to development of infections and is associated with urinary tract infections. The ineffectiveness therapy frequent recurrence these are important reasons for searching alternative treatment strategies. progressive solution this issue has been use oral probiotics a targeted effect on intestinal microflora. AIM: aim study was evaluate antagonistic antibacterial activity probiotic strains Lactobacillus rhamnosus HN001 acidophilus...

Introduction. Uropathogenic Escherichia coli (UPEC) are the dominant bacterial pathogens of urinary tract infections (UTIs). UPEC belong to different phylogenetic groups and have many virulence factors, study which, in conjuction with assessment their relationship clinical forms UTI, is necessary for a better understanding pathogenesis UTI development new diagnostic algorithms. Aim: determination molecular genetic characteristics uropathogenic isolated from pregnant women asymptomatic...

Background. Bacterial films are a marker of chronic recurrent infections. Biofilms on mucous membranes block the inflammatory response macroorganism, suppressing activity immunocytes, and thereby allow microorganisms to reach high concentrations. Currently, research is being conducted find medications that can act biofilms. Enzymes, especially their complexes, substances destroy bacterial films. Objective. Еo determine in vitro sensitivity biofilms formed by vaginal complex enzymes included...

Objective. To study the dynamics and molecular mechanisms of resistance Streptococcus agalactiae strains isolated from pregnant women newborns in St. Petersburg 2010–2022. Materials Methods. The S. (n = 248) were obtained by analyzing clinical material who hospitalized at Research Institute Obstetrics, Gynecology Reproductology named after D.O. Ott 2010–2011 2020–2022. Determination susceptibility to antibacterial drugs was evaluated disk diffusion method. detection genes macrolides...

Abstract Introduction Antimicrobial peptides able to induce immune disorders and inflammation in the uterine cavity are tightly bound microbial composition of upper lower reproductive tract. An interplay between microbiota innate adaptive system is crucial for maintaining a balanced environment embryo implantation processes. Objective: evaluate an endometrial antimicrobial women with different infertility forms. Materials methods A total 181 age were examined. Group I included 165 various...

The endometrium has traditionally been considered sterile. Nowadays, active studies are performed on the female upper genital tract microbiota. Bacteria and/or viruses colonizing known to alter its functional properties, including receptivity and embryo implantation. Uterine cavity inflammation caused by microorganisms leads disrupted cytokine expression, which, in turn, is mandatory for successful implantation of embryo. present study assessed vaginal endometrial microbiota composition...

Bacterial vaginosis is the most common disease of genitals and affects 20–70% women. This non-inflammatory syndrome characterized by dysbiosis vaginal microbiota accompanied a decrease in number lactobacilli. 60% pregnant women asymptomatic, but can lead to obstetric complications such as late miscarriage, premature birth, fetal growth restriction, rupture membranes, choriominionitis, postpartum endometritis, sepsis. The clinical picture bacterial same non-pregnant women, namely homogeneous...