Bacterial vaginosis (BV) is a vaginal disorder characterized by depletion of the normal lactobacillus-dominant microbiota and overgrowth mainly anaerobic bacteria. The study aimed to evaluate distribution abundance Gardnerella vaginalis clades sialidase A gene in samples from Russian women, investigate if G. count detects an abnormal characteristic BV more accurately than load. Vaginal 299 non-pregnant patients gynecological clinics were examined using Nugent scores clade quantitative...

Abstract The large majority of studies investigating associations between bacterial vaginosis (BV) and sexually transmitted infections (STIs) have been conducted among predominantly young women with high risk for STIs. Since a risky sexual behavior is significant factor both STIs BV, this creates bias toward an increased association BV This study evaluated BV-associated vaginal microbiota ( Chlamydia trachomatis , Mycoplasma genitalium Trichomonas vaginalis Neisseria gonorrhoeae ) in...

Background: Vaginal microbiota is a factor that determines woman’s health. Infectious complications of pregnant women, women in labor and newborns are often associated with significant change its composition. Analysis the species diversity vaginal during pregnancy postpartum period primarily contributes to study physiological processes concept “healthy” environment these periods life. Aim: The aim this was evaluate dynamic composition microorganisms before delivery at different times natural...

Background: The role of the microbial factor in genesis early miscarriages (up to 10 weeks pregnancy) has not been established. However, disruption vaginal microbiota may be associated with occurrence late miscarriage. Aim: aim this study was evaluate frequency and determine prognostic significance pregnancy registration. Materials methods: This involved 113 pregnant women registered before gestation, who were divided into two groups. Group I consisted 22 whose ended miscarriage (subgroup IA...

The aims of this study were to compare the performance characteristics and cost-effectiveness pooling endocervical samples for screening diagnosis Chlamydia trachomatis, investigate prevalence C. trachomatis infection in women Leningrad Oblast, Russia. A total 1500 tested individually when pooled groups 5 10 samples, respectively. previously evaluated in-house diagnostic polymerase chain reaction (PCR) assay was utilized. sensitivity specificity PCR not affected by either strategy. estimated...

Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due high costs limited availability of NGS, multiplex real-time PCR draws more attention. The present study aimed evaluate microbial composition dominant lactobacilli species non-pregnant women with bacterial using RT-PCR test its diagnostic significance. In...

Introduction. Colonization of the reproductive organs pregnant women with group B streptococci (GBS; Streptococcus agalactiae) can lead to severe perinatal and neonatal pathology. In modern conditions, aside from prevention antenatal infection fetus during childbirth using antibacterial drugs, vaccination is also necessary. this regard, surveillance GBS genotypes an important task. Objective. To determine molecular genetic determinants virulence agalactiae isolated newborns, monitor...

Objectives Antimicrobial resistance in Mycoplasma genitalium (MG) is a poorly surveyed and controlled global health concern. We evaluated the first commercial dual assay, AmpliSens M. -ML/FQ-Resist-FL for detection of potential macrolide quinolone resistance-associated mutations (MRAMs QRAMs, respectively) estimated prevalence these MG St. Petersburg, Russia. Methods Urogenital samples positive (n=145 from 2007 to 2020) negative (n=56 2021) routine diagnostics were retrospectively analysed...

In the present study, performance of cell culture method, two non‐Russian direct immunofluorescence (DIF) assays, and three different in‐house polymerase chain reaction (PCR) tests used in St. Petersburg, Russia, for detection Chlamydia trachomatis urogenital specimens was evaluated. A total 650 patients were examined it most disquieting that previous C. positivity with Russian DIF assays could – 7 days later be confirmed only 26% women 30% men. Overall, highest diagnostic sensitivity...

Clarification of etiology and identification infectious agents in critically ill newborns is a very urgent problem neonatal resuscitation. The objective . To study the characteristics microbiota at admission to intensive care unit (ICU) specialized hospital. Subjects Methods 165 were examined, bacteriological tests blood, feces, saliva performed. Results Positive culture results obtained 40 (24%) patients. most frequent causative agent was S. epidermidis (47.5%). detection rate E. faecalis...

Microbial biofilms are a problem in many areas of medicine. When the vaginal ecological system is disturbed, well-structured polymicrobial biofilm, covering epithelium and consisting anaerobic bacteria, formed, which may lead to development recurrent bacterial vaginosis. During an ascending infection chronic endometritis, detected endometrium. Biofilms formed by bacteria residing urogenital tract woman, result dysfunctions reproductive system, in-vitro fertilization failures, complications...

Background. Group B streptococci (GBS) are opportunistic bacteria capable of causing severe infections in pregnant women and newborn infants. For effective prevention obstetrical perinatal GBS-associated pathology, investigation into epidemiology GBS carriage diseases, as well elaboration accurate rapid methods for detection necessary.
 Objectives. Validation a molecular test the evaluation frequency risk factors colonization with this microorganism.
 Methods. validation PCR DNA...

The unique physical properties considered of graphene to be especially perspective for photothermal therapy complex, drug address delivery the target organs, and some other fields.Objective objective our study was explore possible impact on microorganism growth as well laboratory animal overall condition.Materials technique: experiments applied samples three concentrations two 15 × mm quartz glasses one which carrying deposited lattice. We have also used 5% blood agar, thioglycollate broth,...

Background: Elevated antibody titers to Chlamydia trachomatis are detected in up 70% of women with tubal factor infertility (TFI). In order evaluate the influence past chlamydial infection on results IVF treatment, a prospective cohort study was performed.Materials and methods: A total 242 TFI were enrolled study. Serum samples (n = 242) follicular fluid 197) analyzed using enzyme-linked immunosorbent assay for anti-C. IgG, IgA, IgM anti-chlamydial heat shock protein 60 kDa IgG.Results:...

Background. Bacterial vaginosis (BV) is disturbance of the vaginal microbiota, characterized by displacement lactobacilli with anaerobic bacteria and capable adversely affecting women’s reproductive health. In development BV, a wide spectrum substantially differing in their properties involved. Grouping bacterial communities into clusters, or types microbiocenosis, might contribute to understanding pathogenic mechanisms elaboration effective tools for diagnostics therapy disease.
 Aim....

Little is known about the susceptibility of posterior segment tissues, particularly human retinal pigment epithelium (hRPE), to Chlamydia trachomatis. The purpose study was investigate possibility infecting hRPE with trachomatis, and examine infectivity different trachomatis clinical isolates for cells cell response infection.Cultured McCoy were inoculated eight (serovar E) at multiplicity infection (MOI) 2.0 or 0.3. To detect samples stained immunohistochemically anti-major outer membrane...

BACKGROUND: A microbial imbalance in the vaginal biotope leads to development of infections and is associated with urinary tract infections. The ineffectiveness therapy frequent recurrence these are important reasons for searching alternative treatment strategies. progressive solution this issue has been use oral probiotics a targeted effect on intestinal microflora. AIM: aim study was evaluate antagonistic antibacterial activity probiotic strains Lactobacillus rhamnosus HN001 acidophilus...

Introduction. Uropathogenic Escherichia coli (UPEC) are the dominant bacterial pathogens of urinary tract infections (UTIs). UPEC belong to different phylogenetic groups and have many virulence factors, study which, in conjuction with assessment their relationship clinical forms UTI, is necessary for a better understanding pathogenesis UTI development new diagnostic algorithms. Aim: determination molecular genetic characteristics uropathogenic isolated from pregnant women asymptomatic...

Background. Bacterial films are a marker of chronic recurrent infections. Biofilms on mucous membranes block the inflammatory response macroorganism, suppressing activity immunocytes, and thereby allow microorganisms to reach high concentrations. Currently, research is being conducted find medications that can act biofilms. Enzymes, especially their complexes, substances destroy bacterial films. Objective. Еo determine in vitro sensitivity biofilms formed by vaginal complex enzymes included...

Objective. To study the dynamics and molecular mechanisms of resistance Streptococcus agalactiae strains isolated from pregnant women newborns in St. Petersburg 2010–2022. Materials Methods. The S. (n = 248) were obtained by analyzing clinical material who hospitalized at Research Institute Obstetrics, Gynecology Reproductology named after D.O. Ott 2010–2011 2020–2022. Determination susceptibility to antibacterial drugs was evaluated disk diffusion method. detection genes macrolides...

Abstract Introduction Antimicrobial peptides able to induce immune disorders and inflammation in the uterine cavity are tightly bound microbial composition of upper lower reproductive tract. An interplay between microbiota innate adaptive system is crucial for maintaining a balanced environment embryo implantation processes. Objective: evaluate an endometrial antimicrobial women with different infertility forms. Materials methods A total 181 age were examined. Group I included 165 various...