A5101627732- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Cancer-related gene regulation
- Fibroblast Growth Factor Research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Proteoglycans and glycosaminoglycans research
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- dental development and anomalies
- Dental Trauma and Treatments
- RNA modifications and cancer
- Periodontal Regeneration and Treatments
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Glutathione Transferases and Polymorphisms
- Renal and related cancers
- Blood Coagulation and Thrombosis Mechanisms
- Kruppel-like factors research
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Sulfur Compounds in Biology
- Respiratory viral infections research
- Glycosylation and Glycoproteins Research
Columbia University
2012-2021
Texas Tech University Health Sciences Center
2018-2019
Texas Tech University
2018-2019
Binzhou Medical University
2016-2019
Columbia University Irving Medical Center
2016
Shanghai Jiao Tong University
2009-2015
Shanghai Children's Hospital
2010
The DiGeorge syndrome, the most common of microdeletion syndromes, affects multiple organs, including heart, nervous system, and kidney. It is caused by deletions on chromosome 22q11.2; genetic driver kidney defects unknown.
Greater levels of prenatal exposure to polycyclic aromatic hydrocarbon (PAH) have been associated with childhood obesity in epidemiological studies. However, the underlying mechanisms are unclear.We hypothesized that PAH over-exposure during gestation would lead weight gain and increased fat mass offspring grand-offspring mice. Further, we altered adipose gene expression DNA methylation genes important adipocyte differentiation be affected.Pregnant dams were exposed a nebulized mixture...
Prenatal exposure to polycyclic aromatic hydrocarbons (PAH) has been associated with sustained effects on the brain and behavior in offspring. However, mechanisms have yet be determined. We hypothesized that prenatal ambient PAH mice would impaired neurocognition, increased anxiety, altered cortical expression of Bdnf Grin2b, greater DNA methylation Bdnf. Our results indicated during open-field testing, exposed offspring spent more time immobile less exploring. Females produced fecal boli....
Scope Maternal consumption of a high‐fat diet (HFD) during pregnancy increases the risk behavioral problems. Folate plays an important role in neuroplasticity and preservation neuronal integrity. This study aims at determining influence diets supplemented with folate on offspring behavior, mechanisms involved. Methods results Female mice were fed control diet, HFD, folate, or HFD for 5 weeks before mating. Open field task elevated plus maze are used to evaluate behaviors. Results showed that...
Abstract Background The interaction between the karyoplast and cytoplast plays an important role in efficiency of somatic cell nuclear transfer (SCNT), but underlying mechanism remains unclear. It is generally accepted that embryos, reprogramming gene expression induced by epigenetic mechanisms does not involve modifications DNA sequences. In cattle, oocytes with various mitochondrial (mtDNA) haplotypes usually have different ATP content can further affect vitro production embryos. As mtDNA...
The purpose of the present study was to evaluate effects platelet‑rich fibrin (PRF) exudate on proliferation, osteogenic differentiation and mineralization human periodontal ligament cells (hPDLCs) in vitro. In PRF obtained with permission, from peripheral blood healthy donors exudates were collected 7th day incubation. hPDLCs premolars, cultured by a tissue explant method identified anti‑vimentin anti‑cytokeratin antibodies. added different concentrations cell proliferation differentiation....
The purpose of this study was to evaluate the effects concentrated growth factor exudate (CGFe) on human periodontal ligament cells (hPDLCs) stimulated by tumor necrosis (TNF)‑α. From peripheral blood healthy donors, CGFe prepared according Sacco protocol after 7 days incubation. hPDLCs were cultured a tissue explant method and identified with anti‑vimentin anti‑cytokeratin antibodies. Cells subjected four different treatments: i) Control; ii) TNF‑α (10 ng/ml); iii) (concentration 50%); iv)...
Heparanase promotes tumor invasion and metastasis in several malignancies including breast cancer. However, the roles regulation mechanisms of heparanase during cancer progression are still not fully understood. The aim this study is to determine differential gene expression specific stages by DNA methylation. We detected levels methylation patterns its promoter cell lines (MCF-7 MDA-MB-435) clinical tissues, respectively. It has been observed that highly expressed invasive MDA-MB-435 cells...
To evaluate antibody responses to panels of Mycobacterium tuberculosis antigens for serological diagnosis active (TB).We cloned, expressed and purified 10 M. recombinant proteins 38KD, MPT32 (M. protein 32), MPT64, EspC (ESX-1 secretion-associated protein), Mtb81, Rv3881, Rv3425, Rv0222, Rv3872 CFP21 (culture filtrate 21), obtained lipoarabinomannan (LAM) polysaccharide antigen from BEI Resources. The plasma immunoglobulin (Ig)G titre the 11 based on 45 patients with pulmonary TB (PTB) 30...
As a crucial pluripotency-related factor, the epigenetic regulation of Oct4 has been studied intensively in mammalians. However, its dynamic changes DNA methylation and histone modification avians remain poorly understood. In present study, we first described alterations acetylation promoter chicken PouV (cPouV; homologue avian) during chick embryonic germ (EG) cell differentiation. The analysis showed that cPouV increased obviously, while decreased dramatically detected by chromatin...
Noncoding RNAs (ncRNAs) have gained widespread attention in recent years as a potentially new and crucial tool biological regulation. Although they been associated with range of developmental processes diseases, knowledge the mechanisms by which act is still surprisingly limited. To claim that almost entire mammalian genome transcribed into functional noncoding transcripts remain controversial. Nevertheless, small number well executed studies on ncRNAs given us important clues concerning...
<b><i>Background:</i></b> Though methotrexate (MTX) is known to inhibit proliferation of trophoblasts derived from ectopic and intrauterine pregnancies, its action on postpartum placenta remains questionable. This study was designed ascertain the efficacy MTX in inducing cell death placental cytotrophoblasts (PPTC). <b><i>Methodology:</i></b> Primary human were isolated placentae 1st 2nd trimester pregnancies placentae. The identified based...
Pharmacognosy Magazine ,2019,15,63,459-465.DOI:10.4103/pm.pm_540_18Published:May 2019Type:Original Article Authors:Xiao-Ming Zhao, Li-Yuan Qu, Zhonghai Yan, Yun Yang, Ying Ma, Zhao-Lun Dong, Zhen-Ming Li, Meng-Wen Xin Wang, and Fei Jiao Author(s) affiliations:Xiao-Ming Zhao1, Qu2, Yan3, Yang4, Ma4, Dong5, Li5, Wang6, Jiao4 1Department of Health Management, PLA 970th Hospital, Yantai, Shandong, China. 2Department Anesthesiology, 3Department Medicine, College Physicians Surgeons, Columbia...
Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression. However, the roles and biological mechanisms miRNA-16-5p in human non-small cell lung (NSCLC) not to be well studied. Here, we validated expression miR-16-5p was decreased significantly NSCLC samples lines. The correlation between clinicopathological features miR-16- 5p showed linked advanced TNM stage, positive lymph node metastasis, short overall survival (OS). Also, a negative...
DNA methylation of 5-methylcytosine (5mC) is a key epigenetic regulator in mammals; the dynamic balance between and demethylation affects transcriptional activity β-globin. However, cytosine β-globin vivo during different stages embryogenesis developing liver has not been fully established. 5-Hydroxymethylcytosine (5hmC) newly discovered modification that presumably generated by oxidation 5mC ten-eleven translocation (TET) family it identified clusters. Here, we determined 5hmC modifications...