A5041455975- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Hemoglobinopathies and Related Disorders
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Mesenchymal stem cell research
- Iron Metabolism and Disorders
- Cancer-related gene regulation
- Blood groups and transfusion
- Cancer-related molecular mechanisms research
- Metabolism and Genetic Disorders
- Chromosomal and Genetic Variations
- Muscle Physiology and Disorders
- DNA and Nucleic Acid Chemistry
- Pluripotent Stem Cells Research
- Genomic variations and chromosomal abnormalities
- Advanced biosensing and bioanalysis techniques
- Neurogenetic and Muscular Disorders Research
- Genetic and phenotypic traits in livestock
- RNA Research and Splicing
- Prenatal Screening and Diagnostics
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
Capital Medical University
2025
Shanghai Children's Hospital
2011-2023
Shanghai Jiao Tong University
2010-2023
National Health and Family Planning Commission
2020
Ministry of Education of the People's Republic of China
2010-2017
Shanghai Institute of Hematology
2006-2011
Monash Institute of Medical Research
2004
State Key Laboratory of Genetic Engineering
2002
Fudan University
2002
Boston Children's Hospital
1991-2001
Despite evidence for the involvement of microRNAs (miRNAs or miRs) in pediatric asthma, mechanism responsible has not yet been fully elucidated. We aimed to identify novel miRNAs and study their pathogenic role(s) children with dust mite-induced asthma order gain a better understanding underlying this disease. For purpose, 62 patients as well age- gender-matched healthy controls were recruited. Twelve pairs subjects randomly subjected microarray-based discovery analysis using miRCURY LNA™...
Abstract Chinese hamster with characteristics is used in experiments, and it of great value the field medical biology research. However, at present, there no high‐efficiency method for evaluating genetic quality hamsters. Here, we developed a novel detection system using single‐nucleotide polymorphism (SNP) markers. To find SNP loci, conducted whole genome sequencing on 24 Then, employed an locus screening criterion that set up previously initially screened 214 loci wide distribution high...
Summary. The β‐thalassaemias represent a heterogenous group of diseases resulting from decreased erythroid β‐globin mRNA expression and imbalanced a/β‐globin chain synthesis which are manifest clinically by ineffective erythropoiesis excessive haemolysis. Increasing levels haemoglobin F (HbF) pharmacological agents has been proposed to ameliorate the severity disease improving balance in globin synthesis. Hydroxyurea (HU), as an effective agent with low toxicity for activating 7‐globin gene,...
Multiplex ligation-dependent probe amplification (MLPA) is widely used to screen genes of interest for deletions and duplications. Since MLPA usually based on size-separation the products, maximum number target sequences that can be screened in parallel limited ∼40. We report design a robust array-based format uses products essentially uniform size (100–120 bp) distinguishes between them by virtue incorporated tag sequences. were thus able increase complexity 124, with very product yields...
The β-thalassemia is associated with abnormality in β-globin gene, leading to imbalanced synthesis of α-/β-globin chains. Consequently, the excessive free α-globin chains precipitate erythrocyte membrane, resulting hemolytic anemia. We have explored post-transcriptional strategies aiming at reduction and enrichment on β 654 ( Hbbth-4/Hbb+ ) mouse, carrying a human splicing-deficient allele Hbbth-4 ). Lentiviral vectors short hairpin RNA (shRNA) targeting and/or antisense facilitating correct...
Epigenetics regulations have an important role in fertilization and proper embryonic development, several human diseases are associated with epigenetic modification disorders, such as Rett syndrome, Beckwith-Wiedemann syndrome Angelman syndrome. However, the dynamics functions of long non-coding RNAs (lncRNAs), one type regulators, pre-implantation development not yet been demonstrated. In this study, a comprehensive analysis mouse early-stage lncRNAs was performed based on public...
To investigate multitissue engraftment of human primitive hematopoietic cells and their differentiation in goats, CD34 + Lin − cord blood transduced with a GFP vector were transplanted into fetal goats at 45–55 days gestation. detected nonhematopoietic organs including blood, bone marrow, spleen, liver, kidney, muscle, lung, heart the recipient (1.2–36% all examined). We identified β2 microglobulin-positive multiple tissues. sorted from perfused liver transplant goat showed insulin-like...
We describe a new point mutation in the SRY gene of Chinese XY female with gonadal dysgenesis (Swyer syndrome). Using double stranded DNA cycle sequencing method, single nucleotide substitution G-->A was identified at codon 113 patient9s gene, resulting conservative amino acid change from alanine (A) to threonine (T) residue that lies within putative binding motif. With this mutation, one MnlI recognition site is abolished and BsmAI present sequence gene; therefore, it easily detected by...
To assess in vivo sequence heterogeneity of the human immunodeficiency virus type 1 (HIV-1) env gene, we used polymerase chain reaction to amplify proviral sequences present peripheral blood mononuclear cell DNA a patient with acquired syndrome (AIDS). The amplified gene fragment (575 bp) contains first hypervariable region and part conserved region. Eleven twelve clones were sequenced, respectively, from specimens collected two months apart. Notable was observed among recovered both...
Both basic research and clinical medicine have urgent demands for highly efficient strategies to simultaneously identify many different DNA sequences within a single tube. Effective simultaneous amplification of multiple target is prerequisite any successful nucleic acid detection method. Multiplex PCR one the best choices this purpose. However, due intrinsic interference competition among primer pairs in same tube, rounds empirical optimization procedures are usually required establish...
Down syndrome (DS) is the most common autosomal aneuploidy caused by trisomy of chromosome 21. Previous studies demonstrated that DS affected mitochondrial functions, which may be associated with abnormal development nervous system in patients DS. Runt-related transcription factor 1 (RUNX1) an encoding gene located on It has been reported RUNX1 affect cell apoptosis via pathway. The present study investigated whether plays a critical role dysfunction and explored mechanism affects functions....
ϕC31 integrase, a site‐specific recombinase, can effectively mediate foreign genes bearing an attB sequence integrated into pseudo attP sites. We have previously identified two sites, BpsF1 and BpsM1 from the bovine genome. In this study, new BF4 BF10, were discovered using half‐nested inverse PCR cow fibroblasts. The genomic locations of these sites by direct sequencing BLAST search, it was confirmed that they reside at positions 4q31 10q35 fluorescence in situ hybridization analysis....
Abstract Background Chromosome abnormalities, especially trisomy of chromosome 21, 13, or 18 as well sex aneuploidy, are a well-established cause pregnancy loss. Cultured cell karyotype analysis and FISH have been considered reliable detectors fetal abnormality. However, results usually not available for 3-4 days more. Multiplex ligation-dependent probe amplification (MLPA) has emerged an alternative rapid technique detection aneuploidies. conventional MLPA does allow relative quantification...
Summary. IVS‐2–654 C → T is a common Chines β‐thalassaemia mutation. Previous studies report that this mutation resulted in the formation of an abnormally spliced mRNA and absence detectable normal β‐globin mRNA, hence was considered to cause βd́; ‐ thalassaemia. We recently used method PCR amplified cDNA copies circulating erythroid cell analyse mutant gene transcripts found does not abolish RNA processing entirely, but significant amount (over 15%) normally processed produced. Microglobin...
Down syndrome (DS), caused by the trisomy of chromosome 21, is one common chromosomal disorders, main clinical manifestations which are delayed nervous development and intellectual disability. Long non‑coding RNAs (lncRNAs) have critical roles in various biological processes, including cell growth, cycle regulation differentiation. The abnormally expressed lncRNAs been previously reported; however, functions regulatory patterns DS remained largely elusive. aim present study was to perform a...
Achondroplasia (ACH) is a genetic disorder with autosomal dominant inheritance and the cause of one most common forms short limb dwarfism in humans. Mutations special sites fibroblast growth factor receptor-3 gene (FGFR3) are reported as ACH, almost 98% cases caused by mutations nucleotide 1138 (Gly380Arg), 97% involving c.1138G>A mutation 1% c.1138G>C mutation. Therefore, development simple, reliable, rapid approach for molecular detection great significance prevention early diagnosis...
AbstractHemoglobin Chongqing is a new slowly-moving and unstable hemoglobin variant with high oxygen affinity, that was discovered in five members of Chinese family the suburb Chongqing. Hemoglobin Harbin another rapidly-moving slightly reduced stability increased found woman living Harbin. The relative amounts these two variants pro-positi were about 9% 18%, respectively. Sequence analyses identified Leu→ARG substitution at position α2(NA2) Hb Chongqing, Lys→MET α16(A14)