A5070877865- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Lung Cancer Research Studies
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Helicobacter pylori-related gastroenterology studies
- Genetics, Aging, and Longevity in Model Organisms
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Veterinary medicine and infectious diseases
- Animal Genetics and Reproduction
- Neurological Disease Mechanisms and Treatments
- Cancer Cells and Metastasis
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Galectins and Cancer Biology
- Cancer therapeutics and mechanisms
- Lipid metabolism and disorders
- Yersinia bacterium, plague, ectoparasites research
Capital Medical University
2016-2025
Capital University
2013-2023
Center for Cancer Research
2022
University of Tennessee Health Science Center
2022
Fourth People’s Hospital of Jinan
2014-2015
The Fourth People's Hospital
2014-2015
Maternally expressed gene 3 (Meg3) encodes a long non-coding RNA that has been shown to play role in tumorigenesis. Skp2 is component of the E3 ubiquitin ligase SCF specifically promotes ubiquitination-associated degradation CDK inhibitor p27, and promote cancer cell growth different types cancers, including non-small lung (NSCLC). Nevertheless, regulatory relationship between Meg3 not acknowledged. Here, we showed NSCLC specimens had significant higher levels significantly lower Meg3,...
Helicobacter pylori (H. pylori, Hp) has been designated a class I carcinogen and is closely associated with severe gastric diseases. During colonization in the mucosa, H. develops immune escape by inducing host tolerance. The epithelium acts as first line of defense against Toll-like receptors (TLRs) epithelial cells being sensitive to components subsequently activating innate system. However, mechanism tolerance induced through TLR signalling pathway not fully elucidated. In this research,...
Abstract Chinese hamster with characteristics is used in experiments, and it of great value the field medical biology research. However, at present, there no high‐efficiency method for evaluating genetic quality hamsters. Here, we developed a novel detection system using single‐nucleotide polymorphism (SNP) markers. To find SNP loci, conducted whole genome sequencing on 24 Then, employed an locus screening criterion that set up previously initially screened 214 loci wide distribution high...
Small cell lung cancer (SCLC) is highly aggressive and prone to hypervascular metastases. Recently, we found profilin 2 (PFN2) expression in SCLC but not normal tissues. Furthermore, PFN2 had been shown promote angiogenesis through exosomes. However, it remains unclear whether contributes the progression metastasis of angiogenesis. We report here that overexpression (OE) increased, whereas its knockdown (KD) decreased proliferation, migration, invasion H446. The exosomes from OE-H446...
Helicobacter pylori is designated as a class I carcinogen of human gastric cancer following long-term infection. During this process, H. bacteria persist in proliferation and death, release bacterial components that come into contact with epithelial cells regulate host cell function. However, the impact exposure to lysate on pathological changes not clear. In study, we aimed investigate regulation mechanisms involved dysfunction continuous lysate. We co-cultured lines GES-1 MKN-45 for 30...
Focal adhesion kinase (FAK) is highly expressed in a variety of human cancers and target for cancer therapy. Since FAK inhibitors only block the activity FAK, they are not effective clinical trials. also functions as scaffold protein kinase-independent pathway. To effectively it required to both kinase-dependent FAK-independent pathways. Thus, we tested new generation drug PROTAC ovarian therapy, which blocks activity. We efficacy its parent inhibitor (VS-6063) cell lines vitro by performing...
Abstract Long noncoding RNAs (lncRNAs) are involved in diabetes related diseases. However, the role of lncRNAs pathogenesis type 2 with macrovascular complication (DMC) has seldomly been recognized. This study screened lncRNA profiles leukocytes from DMC patients and explored protective LYPLAL1-DT endothelial cells (EC) under high glucose (HG) inflammatory conditions (IS). Between healthy controls, 477 differential expression (DE-lncRNAs) were identified. The enrichment pathway analysis...
Cardiovascular diseases (CVD) are the leading cause of death worldwide, wherein myocardial infarction (MI) is most dangerous one. Promoting angiogenesis a prospective strategy to alleviate MI. Our previous study indicated that profilin 2 (PFN2) may be novel target associated with angiogenesis. Further results showed higher levels serum PFN2 and exosomal in patients, mice, pigs In this study, we explored whether endothelial cell (EC)-derived could increase beneficial for treatment Serum PFN2,...
Small cell lung cancer (SCLC) stands as one of the most lethal malignancies, characterized by a grim diagnosis and prognosis. The emergence multi-drug resistance poses significant hurdle to effective therapy. Although previous studies have implicated long noncoding RNA LYPLAL1-DT in tumorigenesis SCLC, precise role highly expressed SCLC chemoresistance underlying mechanism remain inadequately understood.
Background Vascular development, including vasculogenesis and angiogenesis, is involved in many diseases. Cystatin C ( CST 3) a commonly used marker of renal dysfunction, we have previously reported that its expression level associated with variations the gerbil circle Willis. Thus, hypothesized 3 may affect endothelial function angiogenic capacity. In current study, sought to determine influence on explore potential regulatory pathway. Methods Results We analyzed vascular growth factor A...
Objectives We aimed to selectively breed a spontaneous diabetic gerbil when sub-line of inbred showed increased blood glucose levels was found recently. Then we investigated the characteristics including serum insulin, triglyceride, cholesterol, leptin, adiponectin and explored underlying molecular mechanism for phenotype. Methods The line gerbils inbreed by monitoring each animal. adiponectin, leptin were tested using an ELISA kit. expression GLUT4, Akt, calpain 10 (CAPN10) western blot...
The Mongolian gerbil (Meriones unguiculatus), a well-known "multifunctional" experimental animal, plays crucial role in the research of hearing, cerebrovascular diseases and Helicobacter pylori infection. Although whole-genome sequencing gerbils has been recently completed, lack valid gene-editing systems for largely limited further usage biomedical research. Here, efficient targeted mutagenesis was successfully conducted by pronuclear injection with Cas9 protein single-guide RNAs (sgRNAs)...
Deformities in the Circle of Willis (CoW) can significantly increase risk cerebrovascular disease humans. However, molecular mechanisms underlying these deformities have not been understood. Based on our previous studies, variations CoW gerbils are hereditary. A normal is observed approximately 60% gerbils, a percentage that also applies to Thus, gerbil an ideal experimental model for studying CoW. To study variations, we selected genes associated with different types using suppression...
Poultries including chickens, ducks, geese, and pigeons are widely used in the biological medical research many aspects. The genetic quality of experimental poultries directly affects results research. In this study, following electrophoresis analysis short tandem repeat (STR) scanning, we screened out microsatellite loci for determining characteristics Chinese pigeons. panels selected our provide a good choice monitoring population diversity native ducks.
Adipose-derived stem cells (ADSC) are multipotent mesenchymal derived from adipose tissues and capable of differentiating into multiple cell types in the tumor microenvironment (TME). The roles ADSC ovarian cancer (OC) metastasis still not well defined. To understand whether contributes to metastasis, we examined epithelial transition (EMT) markers OC following treatment ADSC-conditioned medium (ADSC-CM). ADSC-CM promotes EMT cells. Functionally, proliferation, survival, migration, invasion....
Abstract Background The Mongolian gerbil is an excellent laboratory animal for preparing the cerebral ischemia model due to its inherent deficiency in circle of Willis. However, low incidence and unpredictability symptoms are caused by numerous complex variant types circle. Additionally, lack evaluation system ischemia/reperfusion (I/R) gerbils has shackled application this model. Methods We created a symptom‐oriented principle detailed neurobehavioral scoring criteria. At different time...
The Mongolian gerbil is a distinctive experimental animal in China, as its genetic qualities possess significant value the field of medical biology research. Here, we aimed to establish an economical and efficient panel for quality detection gerbils using single-nucleotide polymorphism (SNP) markers. To search SNPs, conducted whole-genome sequencing (WGS) 40 from outbred populations. Reliable screening criteria were established preliminarily select SNPs with wide genome distribution high...
The Mongolian gerbil has been a useful laboratory animal in many research fields, especially ischemia studies. However, due to the variation of circle Willis (COW), ischemic model is unstable and various. To solve this problem, we newly established an inbred strain gerbils, restricting breeding keeping F23. data on growth animals are described present study. genetic characteristics F4 F20 detected by microsatellite DNA biochemical markers also shown here. results demonstrated that frequency...
Small cell lung cancer (SCLC) is one of the most malignant tumors that has an extremely poor prognosis. RNA-binding protein (RBP) and long noncoding RNA (lncRNA) have been shown to be key regulators during tumorigenesis as well tumor progression. However, role RBP ELAVL4 lncRNA LYPLAL1-DT in SCLC remains unclear. In this study, we verified acts oncogenic was confirmed vitro vivo. Mechanistically, negatively regulates expression miR-204-5p, leading upregulation PFN2, thus, promoting...
The ELAVL4 (HuD) is a neuron-specific RNA-binding protein expressed in 100% of small cell lung cancer (SCLC) cells and over 50% neuroblastoma cells. aim this study was to investigate the serum HuD concentration SCLC patients possibility its utilization as biomarker cancer.Our included 47 cases 29 normal controls. Indirect competitive inhibition ELISA method established detect antigen samples. To design system, purified real positive negative samples were used, checkerboard titration...