A5074107192- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Pneumonia and Respiratory Infections
- Asthma and respiratory diseases
- bioluminescence and chemiluminescence research
- IL-33, ST2, and ILC Pathways
- Air Quality and Health Impacts
- RNA Interference and Gene Delivery
- Retinal Development and Disorders
- MicroRNA in disease regulation
- Respiratory viral infections research
- Retinal Diseases and Treatments
- Biochemical Analysis and Sensing Techniques
- Neonatal Respiratory Health Research
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Herpesvirus Infections and Treatments
- Preterm Birth and Chorioamnionitis
- Hepatitis B Virus Studies
- RNA and protein synthesis mechanisms
- Retinal and Optic Conditions
- CRISPR and Genetic Engineering
- Advanced Chemical Sensor Technologies
Seventh People's Hospital of Dalian
2024-2025
China Medical University
2025
Shanghai Children's Hospital
2016-2024
Shanghai Jiao Tong University
2019-2024
Guangxi Normal University
2021-2024
Beijing Children’s Hospital
2024
Capital Medical University
2024
Ministry of Education of the People's Republic of China
2023-2024
China Institute of Sport Science
2024
New York State Office for People With Developmental Disabilities
2021-2023
Abstract Leber’s hereditary optic neuropathy (LHON) is a mitochondrially inherited disease leading to blindness. A mitochondrial DNA point mutation at the 11778 nucleotide site of NADH dehydrogenase subunit 4 ( ND4 ) gene most common cause. The aim this study was evaluate efficacy and safety recombinant adeno-associated virus 2 (AAV2) carrying (rAAV2-ND4) in LHON patients G11778A mutation. Nine were administered rAAV2-ND4 by intravitreal injection one eye then followed for 9 months....
Leber's hereditary optic neuropathy (LHON) is a disease that leads to blindness. Gene therapy has been investigated with some success, and could lead important advancements in treating LHON. This was prospective, open-label trial involving 9 LHON patients at Tongji Hospital, Wuhan, China, from August 2011 December 2015. The purpose of this study evaluate the long-term outcomes gene for Nine voluntarily received an intravitreal injection rAAV2-ND4. Systemic examinations visual function tests...
Respiratory syncytial virus (RSV) is a leading cause of severe illness in infants, with no effective treatment. Results phase 2 trial suggested that ziresovir may have efficacy the treatment infants hospitalized RSV infection.
Abstract A genome-wide differential expression of long noncoding RNAs (lncRNAs) was identified in blood specimens autism spectrum disorder (ASD). total 3929 lncRNAs were found to be differentially expressed ASD peripheral leukocytes, including 2407 that upregulated and 1522 downregulated. Simultaneously, 2591 messenger (mRNAs), 1789 821 downregulated, also leukocytes. Functional pathway analysis these revealed neurological pathways the synaptic vesicle cycling, long-term depression...
Despite evidence for the involvement of microRNAs (miRNAs or miRs) in pediatric asthma, mechanism responsible has not yet been fully elucidated. We aimed to identify novel miRNAs and study their pathogenic role(s) children with dust mite-induced asthma order gain a better understanding underlying this disease. For purpose, 62 patients as well age- gender-matched healthy controls were recruited. Twelve pairs subjects randomly subjected microarray-based discovery analysis using miRCURY LNA™...
Background: Anxiety is a common comorbidity in patients with COPD China, and it can significantly decrease patients' quality of life. Almost all anxiety measurements contain somatic items that overlap symptoms side effects medicines, which lead to bias measuring COPD. Therefore, brief disease-specific non-somatic measurement scale, the Inventory for Respiratory Disease (AIR), has been developed validated its English version, needed China. Methods: A two-center study was conducted two...
Mounting evidence has shown that ambient PM2.5 exposure is closely associated with the development of obesity, and adipose tissue represents an important endocrine target for PM2.5. In this study, 3T3-L1 preadipocyte differentiation model was employed to comprehensively explore adipogenic potential After 8 days exposure, adipocyte fatty acid uptake lipid accumulation were significantly increased, cells promoted in a concentration-dependent manner. Transcriptome lipidome analyses revealed...
Definitive structural elucidation of lipids is pivotal for unraveling the functions in biological systems. Despite advancements mass spectrometry (MS) lipid analysis, challenges annotation scope and efficiency remain, especially resolving isomers. Herein, we introduce an optimized method using liquid chromatography coupled with electron impact excitation ions from organic tandem (LC-EIEIO-MS/MS) comprehensive analysis lipids. This approach integrates a six-step analytical protocol precise...
Background microRNAs (miRNAs) are small and non-coding RNAs which play critical roles in physiological pathological processes. A number of methods have been established to detect quantify miRNA expression. However, method for high-throughput function detection is still lacking. Principal Findings We describe an adeno-associated virus (AAV) vector-based microRNA (miRNA) sensor (Asensor) array functional profiling. Each Asensor contains a Gaussia luciferase (Gluc) firefly (Fluc) expression...
Atherosclerosis is a chronic inflammatory vascular disease, and inflammation plays critical role in its formation progression. Elevated serum homocysteine (Hcy) an independent risk factor for atherosclerosis. Previous studies have shown that fatty acid binding protein 4 (FABP4) important macrophage lipid metabolism atherosclerosis induced by Hcy. However, the underlying molecular mechanism of FABP4 Hcy-induced remains unknown. In this study, we found activated Janus kinase 2/signal...
ABSTRACT Adenovirus (Ad) cell attachment is initiated by the of fiber protein to a primary receptor (usually CAR or CD46). This event followed engagement penton base with secondary (integrin) via its loop region, which contains an Arg-Gly-Asp (RGD) motif, trigger virus internalization. To understand well-orchestrated adenovirus process that involves and base, we reconstructed structure Ad5F35 capsid, comprising type 5 (Ad5) capsid pseudotyped Ad35 fiber, at resolution approximately 4.2 Å....
Spontaneous preterm birth (sPTB) occurs before 37 gestational weeks, with premature rupture of the membranes (PPROM) and spontaneous labor (sPTL) as predominant adverse outcomes. Previously, we identified altered expression long non-coding RNAs (lncRNAs) message (mRNAs) related to ubiquitin proteasome system (UPS) in human placentas following pregnancy loss PTB. We therefore hypothesized that similar mechanisms might underlie PPROM. In current study, nine pairs ubiquitin-proteasome-collagen...
Abstract Problem Various etiological factors, such as infection and inflammation, may induce the adverse outcomes of pregnancy miscarriage, stillbirth, or preterm birth. The pathogenic mechanisms associated with these pregnancies are yet unclear. We hypothesized that a common mechanism underlie variant pregnancy, which induced by genetic‐environmental factors. specific objective current study is to uncover molecular mechanism(s) identifying transcripts present in subtypes loss Method Study...
Inverted terminal repeats (ITRs) of the adeno-associated virus (AAV) are essential for rescue, replication, packaging, and integration viral genome. While ITR mutations have been identified in previous reports, we designed a new truncated lacking B-B' C-C' regions named as ITRΔBC investigated its effects on genome expression recombinant AAV (rAAV). The packaging ability was compared between rAAV wild-type (wt) rAAV. Our results showed productivity reduced 4-fold, which is consistent with...
The PI3K-AKT pathway is known to regulate cytokines in dust mite-induced pediatric asthma. However, the underlying molecular steps involved are not clear. In order clarify further steps, this study investigated expression of certain genes and involvement miRNAs pathway, which might affect resultant cytokine-secretion. In-vivo in-vitro ELISA, qRT-PCR microarrays analyses were used study. A down-expression miRNA-27b-3p mite induced asthma group (group D) was found by microarray analysis. This...
Errors in air pollution exposure assessment are often considered as a major limitation epidemiological studies. However, it is difficult to obtain accurate personal level on cohort populations due the prohibitive expense. Personal estimation models used lieu of direct measures but still suffer from issues availability and accuracy. We aim establish PM2.5 model for population assess its performance by applying our subjects. analyzed data representative sites selected subclinical outcomes...