A5101579106- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Olfactory and Sensory Function Studies
- Photosynthetic Processes and Mechanisms
- Corneal surgery and disorders
- Drug-Induced Ocular Toxicity
- Adenosine and Purinergic Signaling
- Metabolism, Diabetes, and Cancer
- Acne and Rosacea Treatments and Effects
- Neonatal Respiratory Health Research
- Glaucoma and retinal disorders
- Mosquito-borne diseases and control
- RNA regulation and disease
- Immune Response and Inflammation
- Redox biology and oxidative stress
- Genomics and Rare Diseases
- Sirtuins and Resveratrol in Medicine
- Cell Adhesion Molecules Research
- Genetics and Neurodevelopmental Disorders
- Signaling Pathways in Disease
- Potato Plant Research
Sichuan University
2024
West China Second University Hospital of Sichuan University
2024
Second Affiliated Hospital of Zhejiang University
2017-2024
Huazhong University of Science and Technology
2009-2022
Tongji Hospital
2009-2020
Shandong First Medical University
2020
Ministry of Ecology and Environment
2019
Ministry of Environmental Protection
2019
Shandong Eye Hospital
2016
Abstract Leber’s hereditary optic neuropathy (LHON) is a mitochondrially inherited disease leading to blindness. A mitochondrial DNA point mutation at the 11778 nucleotide site of NADH dehydrogenase subunit 4 ( ND4 ) gene most common cause. The aim this study was evaluate efficacy and safety recombinant adeno-associated virus 2 (AAV2) carrying (rAAV2-ND4) in LHON patients G11778A mutation. Nine were administered rAAV2-ND4 by intravitreal injection one eye then followed for 9 months....
Leber's hereditary optic neuropathy (LHON) is a disease that leads to blindness. Gene therapy has been investigated with some success, and could lead important advancements in treating LHON. This was prospective, open-label trial involving 9 LHON patients at Tongji Hospital, Wuhan, China, from August 2011 December 2015. The purpose of this study evaluate the long-term outcomes gene for Nine voluntarily received an intravitreal injection rAAV2-ND4. Systemic examinations visual function tests...
Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial mutation with simultaneous or consecutive binocular painless loss of vision and central visual field (VF) defects.1Lam B.L. Feuer W.J. Schiffman J.C. et al.Trial end points natural history in patients G11778A Leber neuropathy: preparation for gene therapy clinical trial.JAMA Ophthalmol. 2014; 132: 428-436Crossref PubMed Scopus (77) Google Scholar In 2008, we initiated recombinant adeno-associated...
To investigate intracerebral insulin resistance and its relationship with tau-protein hyperphosphorylation. A rat model of type 2 diabetes (T2D) was established streptozotocin (STZ). Diabetic rats received intragastric administration pioglitazone (PIO group) or normal saline (T2D for 4 weeks. As a control, non-diabetic (CTL group). The concentrations in cerebrospinal fluid (CSF) blood were determined radioimmunoassay, glucose concentration using oxidation technique. Total phosphorylated...
Purpose. This study was performed to measure the concentration of trans -resveratrol and its three metabolites in human eyes. Methods. The patients who underwent pars plana vitrectomy for rhegmatogenous retinal detachment were included. participants orally given -resveratrol-based supplement (Longevinex®). A suitable amount conjunctiva, aqueous humor, vitreous humor obtained during operation. High-performance liquid chromatography (HPLC) with mass spectrometry (LC/MS/MS) used detect various...
Abstract Genetic factors play an important role in the pathogenesis of diabetic retinopathy (DR). While many studies have focused on genes that increase susceptibility to DR, herein, we aimed explore confer DR resistance. Previously, identified Hmg CoA reductase degradation protein 1 (SYVN1) as a putative protective gene via expression analysis. Transgenic mice overexpressing SYVN1 and wild-type (WT) with streptozotocin-induced diabetes were used this experiment. Retinal damage vascular...
Gene therapy may be a promising approach for the treatment of Leber hereditary optic neuropathy. The aim this study was to evaluate patients with condition who were recruited into an upcoming gene clinical trial and assess any changes in detection parameters provide support trial. Sixteen neuropathy evaluated using visual function tests 12 months before initiation therapy. Then, results acuity (VA), field (VF), RNFL (retinal nerve fiber layer) thickness, Pattern-reversal Visual evoked...
Abstract Background To examine interactions between optic nerves. Methods A total of 24 Sprague–Dawley rats received unilateral intravitreal injections. The were equally divided into four groups: group was administered an adeno‐associated virus ( AAV ) carrying exogenous gene ND 4; rAAV ‐ 4); B , a green fluorescent protein GFP ; ); C fluorogold FG nerve tracer dye; and D phosphate‐buffered saline PBS as control. Two weeks later, expression evaluated in both retinas nerves after frozen...
The objective of this study is to investigate the characteristics and evolution visual field damage caused by Leber's hereditary optic neuropathy (LHON) provide clinical data for diagnosis LHON. Parameters in 32 consecutive patients (49 eyes) with LHON who were confirmed genetic diagnostic tests retrospectively measured within 1 week, between three six months, at months after onset. Visual defects revealed central scotoma 26 eyes (53.1 %), paracentral 12 (24.5 ceco-central 6 (12.2 blind spot...
To investigate the efficacy of intense pulsed light (IPL) combined with deproteinized calf blood extract (DCBE) eye drops for dry disease (DED) patients nociceptive ocular pain.In this prospective, one-center, interventional study, 23 subjects DED and pain were treated a combination IPL DCBE four sessions at four-week interval. Subjective objective assessments on examined analyzed.The visual analog scale (VAS), surface index, assessment survey (OPAS), patient health questionnaire-9 items,...
Abstract Background Beta‐defensin‐2 (BD‐2) plays an important role in host defense against pathogenic microbe challenge by its direct antimicrobial activity and immunomodulatory functions. The present study aimed to determine whether genetic up‐regulation of rat BD‐2 (rBD‐2) could ameliorate chronic Pseudomonas aeruginosa lung infection rats. Methods Plasmid‐encoding rBD‐2 was delivered lungs vivo using linear polyethylenimine at 48 h before challenging with seaweed alginate beads containing...
T-cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3) is preferentially expressed on Th1-helper type T-cells functions to repress the Th1-mediated immune response. However, role of Tim-3 during inflammatory pathogenesis asthma remains unclear. This study determines expression level in CD4+ within peripheral blood bronchoalveolar lavage fluid (BALF) isolated from a murine model atopic explores potential Mice were randomly divided into normal control, day 1, 7 groups, T...
To evaluate an interlaced triple procedure that involved penetrating keratoplasty (PKP), extracapsular cataract extraction (ECCE) using diathermy capsulotomy, and nonopen-sky intraocular lens (IOL) implantation.This retrospective study data from 34 patients who were diagnosed with severe corneal opacities cataracts. These divided into group (21 patients) a traditional (13 patients). In the group, method of continuous curvilinear capsulorhexis (CCC) was completed via capsulotomy. The donor...
Clinical trials of gene therapy for Leber hereditary optic neuropathy (LHON) were conducted in 9 volunteers with the mitochondrial mutation, G11778A ND4. The purpose this study was to investigate whether multilocus mutations directly influence efficacy LHON.Nine LHON participated a clinical trial intravitreal injection an adenoviral vector expressing wild-type Patients subsequently divided into 2 groups: according differences and based on improvements visual acuity. Full DNA sequences groups...
Developmental delay in children under 5 years old, which occurs globally with an incidence of 10%-15%, is caused by multiple factors including genetics, prenatal conditions, perinatal complications, postnatal influences, social factors, and nutritional deficiencies. Gene variants such as
To evaluate the clinical characteristics and viral Colonization of corneas donated by volunteers with coronavirus disease 2019 (COVID-19) before after corneal transplantation.
Pseudo-random number extension and hashing limit the time for encryption decryption in multiple lattice-based post-quantum cryptography (PQC). Keccak is a crucial part pseudo-random hashing, being most restrictive module. With requirement of high-performance, it important to implement configurable core with flexibility high throughput. In this paper, novel structure throughput generator proposed. The method utilizes two-stage series round function circuits reduce cycles half. And benefiting...
OPS 17: Chemical exposures and respiratory outcomes, Room 110, Floor 1, August 26, 2019, 4:30 PM - 5:30 Background: The potential health effects of acrylamide have drawn worldwide attention due to lifelong exposure human. However, the association with lung function underlying mechanisms remain unknown. We aimed investigate exposure-response relationships between urinary metabolites alteration in a general Chinese adult population. Methods: In Wuhan-Zhuhai cohort, including...
This study aims to identify the underlying genetic cause of a Chinese patient with Leber congenital amaurosis (LCA).Detailed clinical data and family history were collected. A medical diagnostic panel sequencing covering 4450 genes was conducted. Two candidate disease-causing mutations detected in CEP290 then validated Sanger bioinformatic analysis. Reverse transcription polymerase chain reaction (RT-PCR) cDNA performed understand effect novel mutation on mRNA splicing.A five-month-old LCA...