A5058924195- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Muscle Physiology and Disorders
- Acute Myeloid Leukemia Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Cardiac Ischemia and Reperfusion
- Nutrition and Health in Aging
- Chronic Lymphocytic Leukemia Research
- GDF15 and Related Biomarkers
- Advanced Radiotherapy Techniques
- Textile materials and evaluations
- Platelet Disorders and Treatments
- Influenza Virus Research Studies
- Chronic Myeloid Leukemia Treatments
- RNA modifications and cancer
- Phenothiazines and Benzothiazines Synthesis and Activities
- Cancer therapeutics and mechanisms
- Autophagy in Disease and Therapy
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Pluripotent Stem Cells Research
- Electrochemical Analysis and Applications
Instituto Português de Oncologia de Coimbra Francisco Gentil
2025
Hospital de São João
2024
University of Lisbon
2021-2023
Instituto Português de Oncologia Francisco Gentil
2023
Instituto de Medicina Molecular João Lobo Antunes
2023
University of Coimbra
2014-2021
Universidade Nova de Lisboa
2021
University of Cambridge
2021
Czech Academy of Sciences, Institute of Biotechnology
2017-2020
Czech Academy of Sciences
2017-2020
Recently, we showed that generation of tumours in syngeneic mice by cells devoid mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition host mtDNA. However, mechanism mtDNA movement between remains unresolved. To determine whether transfer involves whole mitochondria, injected B16ρ0 mouse melanoma into C57BL/6Nsu9-DsRed2 express red fluorescent protein their mitochondria. We document acquired mitochondria from animal, leading normalisation respiration. Additionally, knockdown key...
Nature has evolved intricate machinery to target and degrade RNA, some of these molecular mechanisms can be adapted for therapeutic use. Small interfering RNAs RNase H-inducing oligonucleotides have yielded agents against diseases that cannot tackled using protein-centered approaches. Because are nucleic acid-based, they several inherent drawbacks which include poor cellular uptake stability. Here we report a new approach RNA small molecules, proximity-induced acid degrader (PINAD). We...
MOTS-c, a mitochondrial-derived peptide reduces high-fat-diet-induced muscle atrophy signaling by reducing myostatin expression. The CK2-PTEN-mTORC2-AKT-FOXO1 pathways play key roles in MOTS-c action on
This case report describes the clinical course, diagnosis, and management of a premenopausal woman with history estrogen receptor (ER)-positive human epidermal growth factor 2 (HER2)-positive early breast cancer (EBC) who developed severe thrombocytopenia. After six years diagnosis EBC (treated curative intent), she presented back pain, significant bruising, menorrhagia. During work-up, laboratory tests revealed thrombocytopenia, imaging studies, including computed tomography scan bone...
Abstract Immunotherapy profoundly impacted cancer treatments by harnessing the patient’s immune system. Phagocytosis, process whereby cells engulf and destroy foreign particles or cells, plays a critical role in tumour cell clearance. Herein, we introduce novel concept termed “ENPHASYS” – En hancement of Pha gocytic Sy napse s designed to direct amplify phagocytosis using heterobifunctional molecules named phagocytic synapse enhancers (PSEs). By engineering de novo PD-L1 binder linked...
Abstract p53-mutated tumors often exhibit increased resistance to standard chemotherapy and enhanced metastatic potential. Here we demonstrate that inhibition of dihydroorotate dehydrogenase (DHODH), a key enzyme the de novo pyrimidine synthesis pathway, effectively decreases proliferation cancer cells via induction replication ribosomal stress in p53- checkpoint kinase 1 (Chk1)-dependent manner. Mechanistically, block biogenesis result p53 activation paralleled by accumulation forks...
The authors declare that they have no conflicts of interest.
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) catalyzed the development vaccines and antivirals. Clinically approved drugs against SARS-CoV-2 target virus directly, which makes them susceptible to viral mutations, in turn can attenuate their antiviral activity. Here we report a host-directed (HDA), piperlongumine (PL), exhibits robust activity as result selective induction reactive oxygen species infected cells by GSTP1 inhibition. Using transgenic K18-hACE2...
Bruton's tyrosine kinase (BTK) is a member of the TEC-family kinases and crucial for proliferation differentiation B-cells. We evaluated therapeutic potential covalent inhibitor (JS25) with nanomolar potency against BTK more desirable selectivity inhibitory profile compared to FDA-approved inhibitors ibrutinib acalabrutinib. Structural prediction BTK/JS25 complex revealed sequestration Tyr551 that leads BTK's inactivation. JS25 also inhibited myeloid lymphoid B-cell cancer cell lines. Its...
The emergence of novel variants severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) underscores the need to investigate alternative approaches prevent infection and treat patients with disease 2019. Here, we report preclinical efficacy NL-CVX1, a de novo decoy that blocks virus entry into cells by binding nanomolar affinity high specificity receptor-binding domain SARS-CoV-2 spike protein. Using transgenic mouse model infection, showed single prophylactic intranasal dose NL-CVX1...
<h2>Abstract</h2><h3>Introduction</h3> : Most of radiation oncology centers rely on set-up skin markings for patient setup during treatment delivery. Permanent dark-ink tattooing is the most popular marking method. COMFORTATTOO a unicentric, randomized trial testing two permanent methods: lancets against an electric pen (Comfort Marker 2.0, CM). One substudy was undertaken to test if using CM translates into cosmesis, fading, or satisfaction benefit compared lancets. <h3>Methods</h3>...