A5024192852- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Cancer Genomics and Diagnostics
- Genomics and Phylogenetic Studies
- Diabetes and associated disorders
- Single-cell and spatial transcriptomics
- Sarcoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Job Satisfaction and Organizational Behavior
- Lung Cancer Research Studies
- Diabetes Treatment and Management
- Employee Performance and Management
- Prostate Cancer Treatment and Research
- Mycobacterium research and diagnosis
- Neurofibromatosis and Schwannoma Cases
- Metabolism, Diabetes, and Cancer
- Lymphoma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- FOXO transcription factor regulation
- Congenital heart defects research
- Brain Metastases and Treatment
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Workaholism, burnout, and well-being
- Energy Harvesting in Wireless Networks
University of California, Los Angeles
2023-2024
University of British Columbia
2021-2023
British Columbia Children's Hospital
2020-2023
APLA Health
2023
UCLA Jonsson Comprehensive Cancer Center
2023
Simon Fraser University
2021
Atma Jaya Catholic University of Indonesia
2019
DNA sequencing continues to get cheaper and faster. In parallel, algorithmic innovations have allowed inference of a wide range nuclear, mitochondrial, somatic evolutionary from data. To make automated, high-quality more readily available, we created an extensible Nextflow meta-pipeline called metapipeline-DNA. Metapipeline-DNA supports processing raw reads through alignment, variant detection, quality control subclonal reconstruction. Each step quality-control, data-visualization multiple...
Restoring functional β cell mass is a potential therapy for those with diabetes. However, the pathways regulating are not fully understood. Previously, we demonstrated that Sox4 required proliferation during prediabetes. Here, report regulates through modulating expression of type 2 diabetes (T2D) susceptibility gene GRK5. cell-specific Grk5 knockout mice showed impaired glucose tolerance reduced mass, which was accompanied by upregulation cycle inhibitor Cdkn1a. Furthermore, found may drive...
Abstract Aims/hypothesis Pancreatic islets depend on cytosolic calcium (Ca 2+ ) to trigger the secretion of glucoregulatory hormones and transcriptional regulation genes important for islet response stimuli. To date, there has not been an attempt profile Ca -regulated gene expression in all cell types. Our aim was construct a large single-cell transcriptomic dataset from human exposed conditions that would acutely induce or inhibit intracellular signalling, while preserving biological...
Abstract Reference genomes are foundational to modern genomics. Our growing understanding of genome structure leads continual improvements in reference and new “builds” with incompatible coordinate systems. We quantified the impact build on germline somatic variant calling by analyzing tumour-normal whole-genome pairs against two most widely used human builds. The average individual had a build-discordance 3.8% for SNPs, 8.6% SVs, 25.9% SNVs 49.6% SVs. Build-discordant variants not simply...
High job demand can be a factor that increases work stress and decreases satisfaction, it is expected to employee turnover intention.This research conducted on 50 employees from department store.The purpose of this study examine mediation satisfaction the influence toward employee's intention.The result cannot prove significantly intention through effect stress.Job mediate towards intention.Other results found in show there an satisfaction.
e13108 Background: Breast cancer is the leading cause of cancer-related deaths among women in Brazil and type with highest incidence. When diagnosed at early stages, it has an overall five-year survival rate up to 90%. However, advanced reduced about 24%, 90% stage IV die metastatic complications. Brain one most common sites breast patients, even though a site poor prognosis, mutational profile brain tumors poorly described literature. Therefore, we understand that identification this may...
Abstract Aims/hypothesis Pancreatic islets depend on cytosolic calcium to trigger the secretion of glucoregulatory hormones and regulate transcription genes important for response stimuli. To date, there has not been an attempt profile calcium-regulated gene expression in all islet cell types. Our aim was construct a large single-cell transcriptomic dataset from human exposed conditions that would acutely induce or inhibit intracellular signalling, while preserving biological heterogeneity....
Abstract Cancer is characterized by the ongoing accumulation of somatic mutations, providing selective advantages that may lead to dysregulated cellular proliferation. While cancer genome at diagnosis has been extensively studied, many types still lack strong prognostic biomarkers. The continuous acquisition and selection for driver mutations in a population cells acts as Darwinian process, resulting clonal expansions progressively more aberrant fit phenotypes. Reconstructing tumor evolution...
Abstract Sarcoma is a cancer which originates in the body's connective tissues of mesenchymal origin. These are like bones and muscles that support or protect other parts body. About ~16,000 sarcomas diagnosed annually US, with ~6,700 deaths attributed to disease. Sarcomas typically divided into two major groups: bone soft-tissue sarcomas, each has multiple subtypes. However, because their diversity site-of-origin, vary greatly presentation histological features. Based on The World Health...
Abstract While pancreatic β cells have been shown to originate from endocrine progenitors in ductal regions, it remains unclear precisely where emerge and which transcripts define newborn cells. Here, we used a mouse model “Ins1-GFP;Timer” that provides spatial information during β-cell neogenesis with high temporal resolution. Fluorescent imaging demonstrated that, as expected, some arise close the ducts; unexpectedly, all others away ducts adjacent blood vessels. Single-cell RNA-sequencing...
ABSTRACT Objective Diabetes onset is accompanied with β-cell deficiency, and thus restoring functional mass a promising therapy for those diabetes. However, the regulatory mechanisms controlling are not fully understood. Previously, we demonstrated that transcription factor SOX4 required proliferation in prediabetic state. To elucidate potential by which regulates mass, performed RNA sequencing (RNA-seq) using pancreatic specific knockout mice (βSOX4 KO). The RNA-seq revealed decreased...